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PerCP/Cyanine5.5 anti-mouse CD25 Antibody

Pricing & Availability
Clone
3C7 (See other available formats)
Regulatory Status
RUO
Other Names
IL-2Rα, Ly-43, p55, Tac
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
3C7_PerCPCyanine55_CD25_Antibody_091819.png
ConA + IL-2 stimulated (3 days) C57BL/6 splenocytes stained with CD25 (clone 3C7) PerCP/Cyanine5.5 (filled histogram) or Rat IgG2b, κ PerCP/Cyanine5.5 isotype control (open histogram)
  • 3C7_PerCPCyanine55_CD25_Antibody_091819.png
    ConA + IL-2 stimulated (3 days) C57BL/6 splenocytes stained with CD25 (clone 3C7) PerCP/Cyanine5.5 (filled histogram) or Rat IgG2b, κ PerCP/Cyanine5.5 isotype control (open histogram)
Compare all formats See PerCP/Cyanine5.5 spectral data
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101911 25 µg 100€
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101912 100 µg 259€
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Description

CD25 is a 55 kD glycoprotein, also known as the low affinity IL-2Rα, Ly-43, p55, or Tac. It is expressed on activated T and B cells, thymocyte subset, pre-B cells, and T regulatory cells. In association with CD122 (IL-2Rβ) and CD132(common γ chain), CD25 forms the high affinity signaling IL-2 receptor.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
IL-2-dependent BALB/c mouse helper T-cell clone HT-2
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application Notes

Additional reported applications (for the relevant formats) include: in vitro blocking of IL-2 binding to low- and high-affinity receptors1,2, and immunohistochemical staining of acetone-fixed frozen sections. 3C7 antibody recognizes different epitope of PC61 antibody (Cat. No. 102002). The Ultra-LEAF™Purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 101925 and 101926).

Application References

(PubMed link indicates BioLegend citation)
  1. Ortega RG, et al. 1984. J. Immunol. 133:1970. (Block)
  2. Moreau JL, et al. 1987. Eur. J. Immunol. 17:929. (Block)
Product Citations
  1. Wang X, et al. 2019. Cell Res. 29:787. PubMed
  2. Li M, et al. 2023. BMC Cancer. 23:497. PubMed
  3. Silva M, et al. 2021. Sci Immunol. 6:eabf1152. PubMed
  4. Wei JL, et al. 2021. J Immunother Cancer. 9: . PubMed
  5. Luo W, et al. 2022. Front Immunol. 13:816761. PubMed
  6. Fulham MA, et al. 2019. Am J Physiol Cell Physiol. 317:C687. PubMed
  7. Sordé L, et al. 2017. Immunity, Inflammation, and Disease. 10.1002/iid3.167. PubMed
  8. Bartolacci C, et al. 2018. Cancer Immunol Res. 1.281944444. PubMed
  9. Lei F, et al. 2012. J Vis Exp. 63: 3986. PubMed
RRID
AB_10613642 (BioLegend Cat. No. 101911)
AB_10613642 (BioLegend Cat. No. 101912)

Antigen Details

Structure
Forms high affinity IL-2R with IL-2Rβ (CD122) and IL-2Rγ (CD132), 55 kD
Distribution

Activated T cells and B cells, thymocyte subset, pre-B cells, T regulatory cells

Function
IL-2 receptor, signal transduction
Ligand/Receptor
IL-2
Cell Type
B cells, T cells, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Taniguchi T, et al. 1993. Cell 73:5.
2. Waldmann TA. 1991. J. Biol. Chem. 266:2681.
3. Read S, et al. 2000. J. Exp. Med. 192:295.
4. Lowenthal JW, et al. 1985. J. Immunol. 135:3988.

Gene ID
16184 View all products for this Gene ID
UniProt
View information about CD25 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 2    Revision Date: 09.18.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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