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PerCP/Cyanine5.5 anti-mouse F4/80 Antibody

Pricing & Availability
Clone
BM8 (See other available formats)
Regulatory Status
RUO
Other Names
EMR1, Ly71
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
BM8_PerCPCyanine55_F4-80_Antibody_010319
Thioglycolate-elicited Balb/c mouse peritoneal macrophages were stained with CD11b FITC and F4/80 (clone BM8) PerCP/Cyanine5.5 (left) or rat IgG2a, κ isotype control PerCP/Cyanine5.5 (right).
  • BM8_PerCPCyanine55_F4-80_Antibody_010319
    Thioglycolate-elicited Balb/c mouse peritoneal macrophages were stained with CD11b FITC and F4/80 (clone BM8) PerCP/Cyanine5.5 (left) or rat IgG2a, κ isotype control PerCP/Cyanine5.5 (right).
Compare all formats See PerCP/Cyanine5.5 spectral data
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123127 25 µg 123€
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123128 100 µg 287€
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Description

F4/80, also known as EMR1 or Ly71, is a 160 kD glycoprotein of the epidermal growth factor (EGF)-transmembrane 7 (TM7) family. F4/80 has been widely used as a murine macrophage marker. It is expressed on a majority of tissue macrophages, including macrophages in the lung, gut, peritoneal cavity, thymus, and red pulp of the spleen, Kupffer cells, Langerhans cells, microglia, and certain dendritic cells. It is not expressed on macrophages located in the T cell areas of the spleen, lymph node, or Peyer's patch. The biological ligand of F4/80 has not been identified, but it has been reported that F4/80 is required for the induction of CD8+ T cells-mediated peripheral tolerance.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Murine macrophages
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 1.0 µg per 106 cells in 100 µl. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections1,2 and formalin-fixed paraffin-embedded sections6,7, Western blotting, and spatial biology (IBEX)12,13.

Application References

(PubMed link indicates BioLegend citation)
  1. Schaller E, et al. 2002. Mol. Cell. Biol. 22:8035. (IHC)
  2. Stevceva L, et al. 2001. BMC Clin Pathol. 1:3. (IHC)
  3. Kobayashi M, et al.2008. J. Leukoc. Biol. 83:1354. PubMed
  4. Poeckel D, et al. 2009. J. Biol Chem. 284:21077. PubMed
  5. Glass AM, et al. 2013. J. Immunol. 190:4830. PubMed
  6. Koehm S, et al. 2007. J. Allergy Clin. Immunol. 120:570. (IHC)
  7. Rankin AL, et al. 2010. J. Immunol. 184:1526. (IHC)
  8. Sasi SP, et al. 2014. J Biol Chem. 289:14178. PubMed
  9. Thakus VS, et al. 2014. Toxicol Lett. 230:322. PubMed
  10. Watson NB, et al. 2015. J Immunol. 194:2796. PubMed
  11. Hirakawa H, et al. 2015. PLoS One. 10:119360. PubMed
  12. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  13. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Serra-Hassoun M, et al. 2014. J Immunol. 193:1504. PubMed
  2. Lin D, et al. 2016. Cancer Res . 76: 3179 - 3188. PubMed
  3. Sun L, et al. 2021. Cancer Cell. 39:1361. PubMed
  4. Tong CF, et al. 2018. Am J Physiol Cell Physiol. 315:C409. PubMed
  5. Jaitin DA, et al. 2020. Cell. 178(3):686-698.e14.. PubMed
  6. Bernasconi R, et al. 2021. EMBO Mol Med. :e14392. PubMed
  7. Fu A, et al. 2022. Cell. 185:1356. PubMed
  8. Van Deren DA, et al. 2022. Development. 149: . PubMed
  9. Lee R, et al. 2022. Cancer Discov. 12:1702. PubMed
  10. Serra M, et al. 2022. Cell Mol Gastroenterol Hepatol. 14:609. PubMed
  11. Mansouri S, et al. 2022. J Immunol. 209:2114. PubMed
  12. He Y, et al. 2022. Front Immunol. 13:986202. PubMed
  13. Kawasaki T, et al. 2022. Cell Rep. 41:111828. PubMed
  14. Parida PK, et al. 2022. Cell Metab. 34:90. PubMed
  15. Reticker-Flynn NE, et al. 2022. Cell. 185:1924. PubMed
  16. Boroumand P, et al. 2022. Elife. 11:. PubMed
  17. Shi H, et al. 2022. Nutrients. 14:. PubMed
  18. Wu Z, et al. 2023. EMBO Rep. 24:e56524. PubMed
  19. Li M, et al. 2023. BMC Cancer. 23:497. PubMed
  20. Correa S, et al. 2020. ACS Nano. 14:2224. PubMed
  21. Garcia-Fabiani MB, et al. 2020. Methods Enzymol. 632:369. PubMed
  22. Marco Barros R, et al. 2016. Cell. 167: 203-218. PubMed
  23. Kaku H, et al. 2014. J Immunol. 193:5904. PubMed
  24. Li Q, et al. 2019. Neuron. 101:207. PubMed
  25. Hulsebus HJ, et al. 2022. Front Immunol. 13:884719. PubMed
  26. Zhang Y, et al. 2020. Oncol Lett. 1.053472222. PubMed
  27. Balog S, et al. 2020. Hepatology. 71:291. PubMed
  28. Almeida AS, et al. 2021. Bio Protoc. 11:e4012. PubMed
  29. Schmidleithner L et al. 2019. Immunity. 50(5):1232-1248 . PubMed
  30. Tordesillas L, et al. 2018. Nat Commun. 9:5238. PubMed
  31. Tyagi AM et al. 2018. Immunity. 49(6):1116-1131 . PubMed
  32. Deslyper G, et al. 2021. Parasitology. 148:539. PubMed
  33. Si Y, et al. 2020. Sci Adv. 6:eaba0995. PubMed
  34. Thompson PJ et al. 2019. Cell metabolism. 29(5):1045-1060 . PubMed
  35. Zhong J, et al. 2018. Sci Adv. 4:eaas9864. PubMed
  36. Jones N, et al. 2021. Nat Commun. 12:1209. PubMed
  37. Mul Fedele ML, et al. 2020. Front Cell Infect Microbiol. 10:100. PubMed
  38. Brykczynska U, et al. 2020. Cell Reports. 30(5):1627-1643.e7.. PubMed
  39. Almeida AS, et al. 2021. Bio Protoc. 11:e4012. PubMed
  40. Saint Fleur–Lominy S, et al. 2018. Cell Rep. 24:3045. PubMed
  41. Cong J et al. 2018. Cell metabolism. 28(2):243-255 . PubMed
  42. Zhang P, et al. 2022. BMC Med. 20:435. PubMed
  43. Chen L, et al. 2021. Cell Death Differ. 28:1880. PubMed
  44. Zellweger R, et al. 2010. Cell Host Microbe. 18:128. PubMed
  45. Wang F, et al. 2018. Carcinogenesis. 39:889. PubMed
  46. Si Y, et al. 2018. J Control Release. 282:120. PubMed
  47. Pitsch J, et al. 2021. Ann Neurol. 89:666. PubMed
  48. McFarland AP, et al. 2021. Immunity. 54(6):1320-1337.e4. PubMed
  49. Garg M, et al. 2021. Cell Reports. 34(6):108736. PubMed
  50. Chen Z, et al. 2015. Am J Physiol Endocrinol Metab. 308:460. PubMed
  51. Boehm DT, et al. 2018. Infect Immun. 86:. PubMed
  52. Jiang W, et al. 2021. J Cell Physiol. 236:7711. PubMed
  53. Brandi P, et al. 2022. Cell Rep. 38:110184. PubMed
  54. Momin N, et al. 2022. Nat Commun. 13:109. PubMed
  55. Kren N, et al. 2020. Sci Rep. 10:7390. PubMed
  56. Vogel AB, et al. 2021. Nature. 592:283. PubMed
  57. Macdougall CE et al. 2018. Cell metabolism. 27(3):588-601 . PubMed
  58. Longenecker JZ, et al. 2021. Mol Metab. 54:101343. PubMed
  59. Alsina-Sanchis E, et al. 2020. Mol Cancer Res. . PubMed
  60. Huang D, et al. 2020. Nat Commun. 4.520833333. PubMed
  61. Lim S, et al. 2016. PLoS One. 11: 0155689. PubMed
  62. Sztwiertnia I, et al. 2020. PLoS One. 15:e0233789. PubMed
  63. Song P, et al. 2018. Int J Mol Med. 41:935. PubMed
  64. Bachy S, et al. 2022. iScience. 25:103758. PubMed
  65. RY H, et al. 2016. Oncoimmunology. 6:e1249561. PubMed
  66. Xu P, et al. 2020. Cancer Immunol Res. 8:1193. PubMed
  67. Duan H et al. 2018. Immunity. 49(2):301-311 . PubMed
  68. Mansouri S, et al. 2020. Mucosal Immunol. 0.954861111. PubMed
  69. Traboulsi H, et al. 2015. Antimicrob Agents Chemother. 59: 6317. PubMed
  70. Chou H, et al. 2015. Proc Natl Acad Sci U S A. 112:2175. PubMed
  71. Calderon B, et al. 2015. J Exp Med. 212: 1497-1512. PubMed
  72. Chen X, et al. 2021. Immunity. 54(2):324-339.e8. PubMed
  73. Meunier I, et al. 2015. PLoS One. 10: 0138055. PubMed
  74. Watson N, et al. 2015. J Immunol. 194:2796. PubMed
  75. Buerfent BC, et al. 2019. GMS Infect Dis. 7:Doc04. PubMed
  76. Wu M, et al. 2020. Mucosal Immunol. 13:47. PubMed
  77. Zhao N, et al. 2018. J Clin Invest. 26:84. PubMed
  78. Howard ZM, et al. 2021. Am J Pathol. 191:730. PubMed
  79. Zheng Y, et al. 2022. J Immunol. 208:501. PubMed
  80. Zhang X, et al. 2020. Arterioscler Thromb Vasc Biol. 40:597. PubMed
  81. Lisk C, et al. 2021. Front Immunol. 12:624197. PubMed
  82. Sobecki M, et al. 2022. Cell Stem Cell. 29:1459. PubMed
  83. Kunishita Y, et al. 2020. Front Immunol. 11:98. PubMed
  84. Chen X, et al. 2022. STAR Protoc. 3:101180. PubMed
  85. Zhang XL, et al. 2022. J Am Heart Assoc. 11:e023800. PubMed
  86. Kim SI, et al. 2020. Molecular Cancer Therapeutics. 20(1):173-182. PubMed
  87. Li Z et al. 2018. Immunity. 49(4):640-653 . PubMed
  88. Hwang BJ, et al. 2022. J Immunother Cancer. 10:. PubMed
  89. Kim SH, et al. 2020. Neoplasia. 1.3375. PubMed
  90. Kubli SP, et al. 2019. Nat Commun. 10:2678. PubMed
  91. Rodrigues TS, et al. 2020. J Leukoc Biol. 108:1225. PubMed
  92. Zhang SJ, et al. 2021. Adv Sci (Weinh). 8:2003410. PubMed
  93. Zhong J, et al. 2021. Commun Biol. 4:865. PubMed
  94. Koda S, et al. 2021. Front Immunol. 12:754208. PubMed
RRID
AB_893484 (BioLegend Cat. No. 123127)
AB_893484 (BioLegend Cat. No. 123128)

Antigen Details

Structure
EGF-TM7 family member, 160 kD glycoprotein
Distribution

Majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen, Kupffer cells, Langerhans cells, bone marrow stromal cells, and a subset of dendritic cells

Function
Induction of immunological tolerance
Cell Type
Dendritic cells, Langerhans cells, Macrophages, Tregs
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Antigen References

1. Austy JM and Gordon S. 1981. Eur. J. Immunol. 11:805.
2. Hume DA, et al. 1983. J. Exp. Med. 158:1522.
3. Ruedl C, et al. 1996. Eur. J. Immunol. 26:1801.
4. McKnight AJ, et al. 1996. J. Biol. Chem. 271:486.
5. Lin HH, et al. 2005. J. Exp. Med. 201:1615.

Gene ID
13733 View all products for this Gene ID
UniProt
View information about F4/80 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Other Formats

View All F4/80 Reagents Request Custom Conjugation
Description Clone Applications
Brilliant Violet 605™ anti-mouse F4/80 BM8 FC
Purified anti-mouse F4/80 BM8 FC,IHC,WB
Biotin anti-mouse F4/80 BM8 FC,IHC
FITC anti-mouse F4/80 BM8 FC
PE anti-mouse F4/80 BM8 FC
PE/Cyanine5 anti-mouse F4/80 BM8 FC
PE/Cyanine7 anti-mouse F4/80 BM8 FC
APC anti-mouse F4/80 BM8 FC
APC/Cyanine7 anti-mouse F4/80 BM8 FC
Alexa Fluor® 488 anti-mouse F4/80 BM8 FC,IHC-F,3D IHC
Alexa Fluor® 647 anti-mouse F4/80 BM8 FC,IHC-F,3D IHC
Pacific Blue™ anti-mouse F4/80 BM8 FC
PerCP anti-mouse F4/80 BM8 FC
PerCP/Cyanine5.5 anti-mouse F4/80 BM8 FC
Alexa Fluor® 700 anti-mouse F4/80 BM8 FC
Brilliant Violet 421™ anti-mouse F4/80 BM8 FC,IHC-F,SB
Brilliant Violet 510™ anti-mouse F4/80 BM8 FC
Alexa Fluor® 594 anti-mouse F4/80 BM8 IHC-F
Brilliant Violet 785™ anti-mouse F4/80 BM8 FC
Purified anti-mouse F4/80 (Maxpar® Ready) BM8 FC,CyTOF®
PE/Dazzle™ 594 anti-mouse F4/80 BM8 FC
Brilliant Violet 650™ anti-mouse F4/80 BM8 FC
Brilliant Violet 711™ anti-mouse F4/80 BM8 FC
APC/Fire™ 750 anti-mouse F4/80 BM8 FC
TotalSeq™-A0114 anti-mouse F4/80 BM8 PG
TotalSeq™-B0114 anti-mouse F4/80 BM8 PG
TotalSeq™-C0114 anti-mouse F4/80 BM8 PG
Spark YG™ 570 anti-mouse F4/80 BM8 IHC-F
KIRAVIA Blue 520™ anti-mouse F4/80 BM8 FC
Ultra-LEAF™ Purified anti-mouse F4/80 BM8 FC,IHC,WB
APC/Fire™ 810 anti-mouse F4/80 BM8 FC
Spark NIR™ 685 anti-mouse F4/80 BM8 FC
Spark Blue™ 550 anti-mouse F4/80 BM8 FC
Brilliant Violet 570™ anti-mouse F4/80 BM8 FC
Brilliant Violet 750™ anti-mouse F4/80 BM8 FC
PE/Fire™ 810 anti-mouse F4/80 BM8 FC
Spark Red™ 718 anti-mouse F4/80 (Flexi-Fluor™) BM8 FC
Spark Blue™ 574 anti-mouse F4/80 (Flexi-Fluor™) BM8 FC
Go To Top Version: 3    Revision Date: 01.03.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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