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PerCP/Cyanine5.5 anti-mouse TER-119/Erythroid Cells Antibody

Pricing & Availability
Clone
TER-119 (See other available formats)
Regulatory Status
RUO
Other Names
Ly-76
Isotype
Rat IgG2b, κ
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Product Citations
publications
TER-119_PerCPCy5dot5_011408
C57BL/6 mouse bone marrow cells stained with TER-119 PerCP/Cyanine5.5
  • TER-119_PerCPCy5dot5_011408
    C57BL/6 mouse bone marrow cells stained with TER-119 PerCP/Cyanine5.5
Compare all formats See PerCP/Cyanine5.5 spectral data
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116227 25 µg 95€
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116228 100 µg 259€
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Description

The TER-119 antigen is a 52 kD glycophorin A-associated protein, also known as Ly-76. TER-119 is an erythroid-specific antigen expressed on early proerythroblasts to mature erythrocytes, but not on erythroid colony-forming cells (BFU-E, blast-forming unit erythroid, or CFU-E, colony-forming unit erythroid).

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Day-14 fetal liver cells from a C57BL/6 mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application Notes

The TER-119 antibody is useful for distinguishing erythrocytes and cells in the erythroid lineage. Additional reported applications (for the relevant formats) include: immunoprecipitation1, Western blotting1, complement-mediated cytotoxicity3, and immunohistochemical staining of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections. Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 116253-116258).

Application References

(PubMed link indicates BioLegend citation)
  1. Kina T, et al. 2000. Br. J. Haematol. 109:280. (IP, WB)
  2. Vannucchi AM, et al. 2000. Blood 95:2559.
  3. Maraskovsky E, et al. 1996. J. Exp. Med. 184:1953. (CMCD)
  4. Grisendi S, et al. 2005. Nature 437:147. (FC)
  5. Bourdeau A, et al. 2007. Blood 109:4220.
  6. Chappaz S, et al. 2007. Blood 110:3862. (FC)
  7. Heuser M, et al. 2007. Blood 110:1639. (FC)
  8. Gough SM, et al. 2014. Cancer Discov. 4:564. PubMed
Product Citations
  1. Van Deren DA, et al. 2022. Development. 149: . PubMed
  2. Blomberg OS 2023. Cancer Cell. 41(1):106-123.e10. PubMed
  3. Arimoto KI, et al. 2023. Nat Commun. 14:251. PubMed
  4. Guo H, Cooper S, Friedman A, et al. 2017. PLoS One. 10.1371/journal.pone.0150809. PubMed
  5. Krausgruber T, et al. 2020. Nature. 583:296. PubMed
  6. Gazit R, et al. 2014. J Exp Med. 211:1315. PubMed
  7. Zong L, et al. 2021. NPJ Aging Mech Dis. 7:25. PubMed
  8. De S, et al. 2018. Development. 145. PubMed
  9. Nakamura‐Ishizu A et al. 2018. Cell reports. 25(7):1772-1785 . PubMed
  10. Alcicek FC, et al. 2022. Front Physiol. 13:839323. PubMed
  11. Montel-Hagen A, et al. 2020. Cell Rep. 33:108320. PubMed
  12. Bai Y, et al. 2021. Front Cell Dev Biol. 9:730095. PubMed
  13. JI B, et al. 2016. Cell Death Differ. 23:759-75. PubMed
  14. Kotfis K, et al. 2020. British Journal of Pharmacology. 49(1):66-72. PubMed
  15. Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
  16. Kobayashi M, et al. 2020. Bio Protoc. 10:e3601. PubMed
  17. McFarland AP, et al. 2021. Immunity. 54(6):1320-1337.e4. PubMed
  18. Chauveau A, et al. 2020. Immunity. 52:794. PubMed
  19. Huang D, et al. 2020. Nat Commun. 4.520833333. PubMed
  20. Zhang P, et al. 2022. Nat Commun. 13:1588. PubMed
  21. Matsumura T, et al. 2022. Nat Commun. 13:7064. PubMed
  22. Shaikh H, et al. 2021. Front Immunol. 12:689896. PubMed
  23. Mesnieres M, et al. 2021. Cell Reports. 36(8):109618. PubMed
  24. Dallari S, et al. 2021. Cell Host Microbe. 29(6):1014-1029.e8. PubMed
  25. Böhm AM et al. 2019. Dev Cell. 51(2):236-254 . PubMed
  26. Montel‐Hagen A et al. 2019. Cell stem cell. 24(3):376-389 . PubMed
  27. Tu Z, et al. 2022. Cell Rep. 40:111072. PubMed
  28. Säwen P et al. 2018. eLife. 7 pii: e41258. PubMed
  29. Spiljar M, et al. 2021. Cell Metab. 33:2231. PubMed
  30. Tan DQ, et al. 2019. Cell Rep. 26:2316. PubMed
RRID
AB_893636 (BioLegend Cat. No. 116227)
AB_893636 (BioLegend Cat. No. 116228)

Antigen Details

Structure
Associated with glycophorin A, 52 kD
Distribution

Early proerythroblast to mature erythrocyte, but not BFU-E and CFU-E

Cell Type
Erythrocytes
Biology Area
Immunology
Antigen References

1. Kina T, et al. 2000. Br. J. Haematol. 109:280.
2. Ikuta K, et al. 1990. Cell 62:863.
3. Osawa M, et al. 1996. Weir's Handbook of Experimental Immunology. Vol. 2 pp. 66.1-66.5.

Gene ID
104231 View all products for this Gene ID
UniProt
View information about TER-119 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Other Formats

View All TER-119 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse TER-119/Erythroid Cells TER-119 FC
Biotin anti-mouse TER-119/Erythroid Cells TER-119 FC
FITC anti-mouse TER-119/Erythroid Cells TER-119 FC
PE anti-mouse TER-119/Erythroid Cells TER-119 FC
PE/Cyanine5 anti-mouse TER-119/Erythroid Cells TER-119 FC
Purified anti-mouse TER-119/Erythroid Cells TER-119 FC,IHC-F,IHC-P,IP,WB
Alexa Fluor® 488 anti-mouse TER-119/Erythroid Cells TER-119 FC
Alexa Fluor® 647 anti-mouse TER-119/Erythroid Cells TER-119 FC
Alexa Fluor® 700 anti-mouse TER-119/Erythroid Cells TER-119 FC
PE/Cyanine7 anti-mouse TER-119/Erythroid Cells TER-119 FC
APC/Cyanine7 anti-mouse TER-119/Erythroid Cells TER-119 FC
PerCP anti-mouse TER-119/Erythroid Cells TER-119 FC
PerCP/Cyanine5.5 anti-mouse TER-119/Erythroid Cells TER-119 FC
Brilliant Violet 421™ anti-mouse TER-119/Erythroid Cells TER-119 FC
Pacific Blue™ anti-mouse TER-119/Erythroid Cells TER-119 FC
Brilliant Violet 650™ anti-mouse TER-119/Erythroid Cells TER-119 FC
Brilliant Violet 510™ anti-mouse TER-119/Erythroid Cells TER-119 FC
Brilliant Violet 605™ anti-mouse TER-119/Erythroid Cells TER-119 FC
Purified anti-mouse TER-119/Erythroid Cells (Maxpar® Ready) TER-119 FC,CyTOF®
PE/Dazzle™ 594 anti-mouse TER-119/Erythroid Cells TER-119 FC
Brilliant Violet 785™ anti-mouse TER-119/Erythroid Cells TER-119 FC
TotalSeq™-A0122 anti-mouse TER-119/Erythroid Cells TER-119 PG
APC/Fire™ 750 anti-mouse TER-119/Erythroid Cells TER-119 FC
TotalSeq™-B0122 anti-mouse TER-119/Erythroid Cells TER-119 PG
TotalSeq™-C0122 anti-mouse TER-119/Erythroid Cells TER-119 PG
Ultra-LEAF™ Purified anti-mouse TER-119/Erythroid Cells TER-119 FC,IHC-F,IHC-P,IP,WB,CMCD
Spark Blue™ 550 anti-mouse TER-119/Erythroid Cells TER-119 FC
APC/Fire™ 810 anti-mouse TER-119/Erythroid Cells TER-119 FC
Spark NIR™ 685 anti-mouse TER-119/Erythroid Cells Antibody TER-119 FC
Brilliant Violet 711™ anti-mouse TER-119/Erythroid Cells TER-119 FC
Spark Red™ 718 anti-mouse TER-119/Erythroid Cells (Flexi-Fluor™) TER-119 FC
Spark Blue™ 574 anti-mouse TER-119/Erythroid Cells (Flexi-Fluor™) TER-119 FC
Go To Top Version: 2    Revision Date: 12.04.2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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