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PerCP/Cyanine5.5 anti-mouse CD90.2 (Thy1.2) Antibody

Pricing & Availability
Clone
53-2.1 (See other available formats)
Regulatory Status
RUO
Other Names
Thy-1.2
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
53-21_PerCPCyanine55_CD902_Antibody_FC_010319
C57BL/6 mouse splenocytes were stained with CD3e APC and CD90.2 (clone 53-2.1) PerCP/Cyanine5.5 (left), or rat IgG2a, κ PerCP/Cyanine5.5 isotype control (right).
  • 53-21_PerCPCyanine55_CD902_Antibody_FC_010319
    C57BL/6 mouse splenocytes were stained with CD3e APC and CD90.2 (clone 53-2.1) PerCP/Cyanine5.5 (left), or rat IgG2a, κ PerCP/Cyanine5.5 isotype control (right).
Compare all formats See PerCP/Cyanine5.5 spectral data
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140321 25 µg 81€
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140322 100 µg 156€
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Description

CD90.2 is a 25-35 kD immunoglobulin superfamily member also known as Thy1.2, a GPI-linked membrane molecule. It is expressed on hematopoietic stem cells and neurons, all thymocytes, and peripheral T cells in Thy1.2 bearing mouse strains (Balb/c, CBA/J, C3H/He, C57BL/-, DBA, NZB/-). CD90.2 is a glycosylphosphatidylinositol (GPI)-anchored membrane glycoprotein involved in signal transduction. CD90.2 is involved in costimulation of lymphocyte proliferation and induction of hematopoietic stem cells differentiation. CD90.2 has been shown to interact with CD45.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse thymus or spleen
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining1 of frozen tissue section, immunofluorescence2, and immunoprecipitation3. Does not react with Thy-1.1 (CD90.1).

Application References

(PubMed link indicates BioLegend citation)
  1. Aldrich M, et al. 2003. J. Immunol. 171:5562. (IHC)
  2. Jameson J, et al. 2004. J. Immunol. 172:3573. (IF)
  3. Okada C, et al. 1990. J. Immunol. 144:3473. (IP)
Product Citations
  1. Logan K Smith et al. 2018. Immunity. 48(2):299-312 . PubMed
  2. Dragan M, et al. 2022. J Invest Dermatol. 142:583. PubMed
  3. Mogilenko DA, et al. 2020. Immunity. 54(1):99-115.e12. PubMed
  4. Wang X, et al. 2020. Signal Transduct Target Ther. 5:35. PubMed
  5. Mathur S, et al. 2021. JCI Insight. 6:. PubMed
  6. Boothby IC, et al. 2021. Nature. 599:667. PubMed
  7. Ku C, et al. 2015. Genes Dev. 29: 1930-1941. PubMed
  8. Kolev M, et al. 2020. Immunity. 52(3):513-527. PubMed
  9. Ko HJ, et al. 2020. Front Immunol. 1897:11. PubMed
  10. Wang D, et al. 2022. EMBO Rep. 23:e53691. PubMed
  11. Dallari S, et al. 2021. Cell Host Microbe. 29(6):1014-1029.e8. PubMed
  12. Di Pilato M, et al. 2021. Cell. 184(17):4512-4530.e22. PubMed
  13. Lal JC, et al. 2021. Breast Cancer Res. 23:83. PubMed
RRID
AB_2562696 (BioLegend Cat. No. 140321)
AB_2562696 (BioLegend Cat. No. 140322)

Antigen Details

Structure
Ig superfamily, 25-35 kD
Distribution

Hematopoietic stem cells and neurons, epithelial cells, fibroblasts, neurons, all thymocytes, and peripheral T cells of Thy-1.2 bearing mice

Function
Lymphocyte costimulation, proliferation, and differentiation of hematopoietic stem cells
Ligand/Receptor
CD45
Cell Type
Epithelial cells, Fibroblasts, Hematopoietic stem and progenitors, Neurons, T cells, Thymocytes
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Signal Transduction
Molecular Family
CD Molecules
Antigen References

1. Borrello M, et al. 1996. Cell. Immunol.173:198.
2. Radrizzani M, et al. 1995. J. Neurosci. Res. 42:220.
3. Williams A, et al. 1982. Science 216:696.

Gene ID
21838 View all products for this Gene ID
UniProt
View information about CD90.2 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 2    Revision Date: 01.03.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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