Propidium Iodide Solution

Product Details

Formulation

Phosphate-buffered saline, pH 7.2, containing 0.09% sodium azide.

Concentration

0.5 mg/ml

Storage & Handling

The solution should be stored undiluted between 2°C and 8°C, and protect from light. Caution: This solution contains hazardous material; handle with care.

Application

FC - Quality tested

Recommended Usage

The suggested use of this solution for viability staining is 10 µl per million cells in 0.5 ml/test, and incubate for 15 minutes at 4 °C before analysis. For Cell Cycle analysis, please see our Propidium Iodide Cell Cycle Staining Protocol. Caution: This solution is toxigenic and mutagenic; handle with care.

Excitation Laser

Blue Laser (488 nm)

Application Notes

Propidium Iodide Solution can be used in evaluation of apoptosis, cell viability and cell cycle analysis by flow cytometry.

Application References

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See More

Antigen Details

Biology Area

Cell Biology, Cell Cycle/DNA Replication, Neuroscience

Gene ID

NA

Documentation

• Technical data sheet
• Certificate of Analysis
• Safety Data Sheet

Reviews

Related Protocols

• Propidium Iodide Cell Cycle Staining Protocol
• Cell Surface Flow Cytometry Staining Protocol

Related Pages & Pathways

Related FAQs

Why is washing not recommended after the addition 7-AAD or PI addition when assessing viability?

These dyes bind in equilibrium with DNA. Therefore, external dye concentration must be maintained during analysis and the dye should not be washed out.

Certificate of Analysis