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Purified anti-14-3-3 ζ/δ Antibody

Pricing & Availability
Clone
W15121A (See other available formats)
Regulatory Status
RUO
Other Names
Protein kinase C inhibitor protein 1, KCIP-1, HEL4, YWHAD, HEL-S-3, HEL-S-93, 14-3-3-zeta
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
W15121A_Purified_14dash3dash3_zetaslashdelta_Antibody_1_WB_082316
Total cell lysates (15 µg protein) from Molt-4 (lane 1), PC3 (lane 2), HeLa (lane 3) and THP-1 (lane 4) were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1 µg/mL (1:500 dilution) of purified anti-14-3-3 ζ/δ antibody, clone W15121A (upper panel). Proteins were visualized by chemiluminescence detection (Cat. No 426303) using a 1:3000 diluted anti-rat-IgG secondary antibody conjugated to HRP for anti-14-3-3 ζ/δ antibody or 1:5000 diluted Direct-Blot HRP anti-β-Actin antibody, clone 2F1-1 (lower panel). Lane M: Molecular weight ladder.
  • W15121A_Purified_14dash3dash3_zetaslashdelta_Antibody_1_WB_082316
    Total cell lysates (15 µg protein) from Molt-4 (lane 1), PC3 (lane 2), HeLa (lane 3) and THP-1 (lane 4) were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1 µg/mL (1:500 dilution) of purified anti-14-3-3 ζ/δ antibody, clone W15121A (upper panel). Proteins were visualized by chemiluminescence detection (Cat. No 426303) using a 1:3000 diluted anti-rat-IgG secondary antibody conjugated to HRP for anti-14-3-3 ζ/δ antibody or 1:5000 diluted Direct-Blot HRP anti-β-Actin antibody, clone 2F1-1 (lower panel). Lane M: Molecular weight ladder.
  • W15121A_Purified_14dash3dash3_zetaslashdelta_Antibody_2_IF_062816
    HeLa cells were fixed with methanol for five minutes and blocked with 1% BSA for 60 minutes. Then the cells were intracellularly stained with 2.0 µg/ml purified anti-14-3-3 ζ/δ (clone W15121A) antibody for one hour, followed by staining with Alexa Fluor® 594 conjugated anti-rat IgG antibody (red) and DAPI (blue) for one hour at room temperature. The image was captured with a 60X objective.
  • W15121A_Purified_14dash3dash3_zetaslashdelta_Antibody_3_WB_082719
    Western blot of purified anti-14-3-3 ζ/δ antibody (clone W15121A). Lane 1: Molecular weight marker; Lane 2: 20 µg of Drosophila head lysate; Lane 3: 20 µg of Drosophila S2 (embryonic) cell lysate. The blot was incubated with 0.25 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-rat IgG (Cat. No. 405405). Enhanced chemiluminescence was used as the detection system.
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687802 100 µg 184€
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Description

In mammals, proteins of the 14-3-3 family include isoforms β, γ, ε, σ, ζ, τ, and η. 14-3-3 δ isoform is the phosphorylated form of 14-3-3 ζ. These highly conserved proteins form homo- or heterodimers with many transcription factors, metabolic enzymes, kinases, phosphatases, and other signaling molecules, generally through a phospho-Ser/Thr motif. The binding regulates the location, stability, interaction with other molecules, and phosphorylation status of the target proteins which suggests the important roles for these 14-3-3 family proteins on regulation of cellular functions and responses. Growing data demonstrate that the specific temporal and spatial distributions of 14-3-3 family proteins are critical for developmental processes; and in some cancers, the regulation of these proteins are different from healthy controls. 14-3-3 ζ interacts with IRS1 protein and is involved in the PI3K-Akt signaling pathway.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse, Rat, Drosophila
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Full length human 14-3-3 ζ/δ recombinant protein expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 – 2.0 µg/ml in human, mouse, and rat and 0.25 – 2.0 µg/ml in Drosophila. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

14-3-3 family members share a high homology in amino acid sequences. Hence, this clone may recognize other 14-3-3 family members with predicted MW of 27, 28kD.

Reactivity to Drosophila was only verified with the purified format.

RRID
AB_2617006 (BioLegend Cat. No. 687802)

Antigen Details

Structure
245 amino acids with a predicted molecular weight of 27.7 kD.
Distribution

Cytoplasm.

Function
14-3-3 ζ/δ is an adaptor protein, binding to a large number of Ser/Thr phosphorylated proteins. It participates in several signaling pathways and regulates the activity of the binding partners.
Interaction
Forms a homooligomer or a heterodimer with other isoforms. Interacts with CDK16, BSPRY, WEE1, SAMSN1, MLF1, ITGB2, BCL2L11, FOXO4, NOXA1, SSH1, ARHGEF2, BAX, RAF1, TP53, ABL1, AANAT, AKT1, GAB2, TLK2, and DAPK2.
Biology Area
Cell Biology, Signal Transduction
Antigen References

1. Funami K, et al. 2016. Mol. Immunol. 73:60.
2. Bonet R, et al. 2013. J. Mol. Biol. 425:3060.
3. Hong HY, et al. 2010. Mol. Cells 29:305.
4. Danes CG, et al. 2008. Cancer Res. 68:1760.
5. Niemantsverdriet M, et al. 2008. Oncogene. 27:1315.
6. Bialkowska K, et al. 2003. J. Biol. Chem. 278:33342.

Gene ID
7534 View all products for this Gene ID
UniProt
View information about 14-3-3 zeta/delta on UniProt.org
Go To Top Version: 3    Revision Date: 08.27.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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