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Purified anti-6-His Epitope Tag Antibody (Previously Covance catalog# MMS-156P)

Pricing & Availability
Clone
6-His (See other available formats)
Regulatory Status
RUO
Other Names
polyhistidine-tag, hexa histidine-tag, 6xHis-tag, His6 tag, His-tag(TM)
Previously
Covance Catalog# MMS-156P
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
1_6-His_Purified_6-His_Antibody_WB_021715
Western Blot showing specificity of 6-His Monoclonal Antibody, Purified, 1:900. Lane 1: MW Marker, Lane 2: PosiTag lysate, Lane 3: Hela cell lysate negative control.
  • 1_6-His_Purified_6-His_Antibody_WB_021715
    Western Blot showing specificity of 6-His Monoclonal Antibody, Purified, 1:900. Lane 1: MW Marker, Lane 2: PosiTag lysate, Lane 3: Hela cell lysate negative control.
  • 2_6-His_Purified_6-His_Antibody_ICC_011421
    Hela cells transfected with multiple tag plasmid were fixed with cold methanol at -20°C for 15 minutes and blocked with 5% FBS for 60 minutes. The cells were then intracellularly stained with either (A) 2 µg/mL anti-mouse IgG1, κ or (B) 2 µg/mL anti-6-His antibody (clone 6-His), or (C) 0.5 µg/mL anti-6-His antibody (clone 6-His), or (D) 0.2 µg/mL anti-6-His antibody (clone 6-His), and incubated overnight at 4°C followed by 2µg/ml (1:250) Alexa Fluor® 594 (Red) goat anti-mouse IgG for one hour at room temperature. Nuclei were counterstained with DAPI (Blue). The image was captured with a 60X objective. Exposure times: (A) 1/25, (B) 1/80, (C) 1/80, (D) 1/80.
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906113 25 µL 72€
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906101 200 µL 285€
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906102 500 µL 445€
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Description

His tags are commonly used as a method of tagging proteins for detection or purification. This antibody is raised again a polyhistidine tag (6-His or hexa His-tag).

Product Details
Technical data sheet

Product Details

Verified Reactivity
Epitope tag
Reported Reactivity
Species independent
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Monoclonal 6-His antibody was raised against a recombinant protein containing the sequence HHHHHH.
Formulation
Phosphate-buffered solution + 0.03% Thimerosal.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/mL
Storage & Handling
Store between 2°C and 8 °C.
Application

WB - Quality tested
ICC, IP - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1:1000 dilution. For immunocytochemistry, the suggested use is 1:1000 – 1:4000 dilution. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The sequence of the protein flanking the poly His tag does not influence antibody binding. This antibody was subsequently purified via a protein-G column.

Application References

(PubMed link indicates BioLegend citation)
  1. Deinert K, et al. 2001. J. Biol. Chem. 276:6000. (WB)
  2. Kojima H, et al. 2001. J. Biol. Chem.(22)276:19238. (IF) PubMed
  3. Lapinski PE, et al. 2000. J. Biol. Chem. 275:6831. (WB)
  4. Bartsch D, et al. 1998. Cell. 95:211. (IF)
  5. Fuentes EJ, et al. 2003. J Biol Chem. 278:2118. PubMed
  6. Montegna EA, et al. 2012 PLoS One.7:31156. (WB) PubMed
  7. Huang L, et al. 2013. Nat Biotechnol. 31:450. (WB) PubMed
Product Citations
  1. Dotiwala F, et al. 2016. Nat Med. 22:210-216. PubMed
  2. Xia T, et al. 2023. PLoS Pathog. 19:e1011232. PubMed
  3. Montegna E, et al. 2012. PLoS One. 7:e31156. PubMed
  4. Marshall RS, et al. 2020. J Cell Sci. 133:jcs249862. PubMed
  5. Huang L, et al. 2013. Nat Biotechnol. 31:350-356. PubMed
  6. Sánchez-Bellver L, et al. 2022. Int J Mol Sci. 23:. PubMed
  7. Marques PE et al. 2019. Dev Cell. 50(3):283-295 . PubMed
  8. Mukherjee S et al. 2019. Developmental cell. 49(1):63-76 . PubMed
  9. Fuentes E, et al. 2003. J Biol Chem. 278:21188-21196. PubMed
  10. Marshall RS, et al. 2020. J Cell Sci. :133. PubMed
  11. Kojima H, et al. 2001. J Biol Chem. 276:19238-19243. PubMed
RRID
AB_2565061 (BioLegend Cat. No. 906113)
AB_2565061 (BioLegend Cat. No. 906101)
AB_2565061 (BioLegend Cat. No. 906102)

Antigen Details

Biology Area
Cell Biology
Gene ID
NA
UniProt
View information about 6-His on UniProt.org
Go To Top Version: 5    Revision Date: 09.19.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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