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Purified anti-AICD Antibody (Previously Covance catalog# SIG-39148)

Pricing & Availability
Clone
Poly8119 (See other available formats)
Regulatory Status
RUO
Other Names
Amyloid beta A4 protein, preA4, protease nexin-II, peptidase nexin-II, beta-amyloid peptide, alzheimer disease amyloid protein, cerebral vascular amyloid peptide, APP, Amyloid Precursor Protein
Previously
Signet Catalog# 9148-005
Covance Catalog# SIG-39148
Isotype
Rabbit Polyclonal IgG
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Product Citations
publications
Poly8119_PURE_AICD_Antibody_071621.png
Direct ELISA of purified anti-AICD antibody (clone Poly8119) binding to plate-immobilized recombinant CTF-50 (blue, positive control) and CTF-57 (red, negative control) proteins. ELISA was performed by coating wells with 0.5 µg/mL of recombinant proteins. The wells were then incubated with the primary antibody at room temperature for 2 hours, followed by incubation with HRP Donkey anti-rabbit IgG antibody (Cat. No. 405405). TMB (3, 3', 5, 5' tetramethylbenzidine, Cat. No. 421501) Substrate solution was used as the detection system.
  • Poly8119_PURE_AICD_Antibody_071621.png
    Direct ELISA of purified anti-AICD antibody (clone Poly8119) binding to plate-immobilized recombinant CTF-50 (blue, positive control) and CTF-57 (red, negative control) proteins. ELISA was performed by coating wells with 0.5 µg/mL of recombinant proteins. The wells were then incubated with the primary antibody at room temperature for 2 hours, followed by incubation with HRP Donkey anti-rabbit IgG antibody (Cat. No. 405405). TMB (3, 3', 5, 5' tetramethylbenzidine, Cat. No. 421501) Substrate solution was used as the detection system.
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811901 50 µg 423€
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Description

APP intracellular domain (AICD) is a peptide fragment that results from intramembraneous proteolysis of the amyloid precursor protein (APP). APP is a single-pass transmembrane protein that is involved in the pathophysiology of Alzheimer's disease (AD). APP is cleaved by gamma-secretase that releases the AICD into the cytoplasm. Soon after its release from the membrane, AICD is rapidly degraded in the cytoplasm partly by insulin degrading enzymes.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Polyclonal
Host Species
Rabbit
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1.0 mg/mL
Storage & Handling
Do not store antibody diluted below 50 µg/mL in the absence of protein (i.e. add 2% bovine serum albumin). The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

ELISA
ICC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA applications, a concentration range of 0.01 - 1 µg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

 

Application Notes

This antibody is specific for AICD (APP intracellular domain), which is a peptide fragment that results from the cleavage of C83 or C99 at the epsilon site. It does not react with the C57 or C59 γ-secretase cleavage products of APP.

This product may contain other non-IgG subtypes.

Application References

(PubMed link indicates BioLegend citation)
  1. Kaori K, et al. 2012. Invest Opththalmol Vis Sci. 53:7675-83. (WB)
  2. Stieren ES, et al. 2011. J Biol Chem. 286:35689-98. (WB)
  3. Tampellini D, et al. 2011. J Neurosci. 31:15384-90. (IF) PubMed
Product Citations
  1. Tampellini D, et al. 2011. J Neurosci. 31:15384-15390. PubMed
  2. Shang Y, et al. 2022. Acta Neuropathol. 144:911. PubMed
  3. Hicks DA, et al. 2020. Bioscience Reports. 40(4):. PubMed
RRID
AB_2564761 (BioLegend Cat. No. 811901)

Antigen Details

Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
APP/β-Amyloid
Gene ID
351 View all products for this Gene ID
UniProt
View information about AICD on UniProt.org
Go To Top Version: 8    Revision Date: 01.25.2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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