- Clone
- E5206 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- MIWC, WCH4, AQP4
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![E5206_PURE_-Aquaporin-4_Antibody_120721 E5206_PURE_-Aquaporin-4_Antibody_120721](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/E5206_PURE_-Aquaporin-4_Antibody_120721-.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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IHC staining of anti-Aquaporin-4 antibody (clone E5206) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928502), the tissue was incubated with 5.0 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin HRP Kit (Multi-Species, DAB) (Cat. No. 929501) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 μm -
IHC staining of anti-Aquaporin-4 antibody (clone E5206) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928502), the tissue was incubated with 5.0 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin HRP Kit (Multi-Species, DAB) (Cat. No. 929501) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 μm -
IHC staining of anti-Aquaporin-4 antibody (clone E5206) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928502), the tissue was incubated with 5.0 µg/mL of purified anti-Aquaporin-4 antibody and Alexa Fluor® 488 anti-GFAP antibody (Cat. No. 837507) overnight at 4°C. Alexa Fluor® 594 Goat anti-mouse IgG antibody (Cat. No. 405326) was used for 2-hour incubation at room temperature, followed by nuclear counterstaining with DAPI (Cat. No. 422801). The slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scale bar: 50 μm
Cat # | Size | Price | Quantity Check Availability | Save | ||
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600351 | 25 µg | 95€ | ||||
600352 | 100 µg | 235€ |
Aquaporin-4 (AQP4) is a member of the water-selective channel family that is predominantly expressed in astrocytes in the central nervous system. AQP4 is a regulator of water homeostasis by exchanging water across the endfoot membranes in the brain. This protein is vital for maintaining the normal level of cerebrospinal fluid influx into the brain cortex, parenchyma, and perivascular space.
Product DetailsProduct Details
- Verified Reactivity
- Human, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Partial recombinant human AQP4 protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
IHC-P - Quality tested
ICC, IHC-F, WB, IP - Reported in the literature, not verified in house - Recommended Usage
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Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
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Application References
(PubMed link indicates BioLegend citation) -
- Ramadhanti J, et al. 2013. Monoclon Antib Immunogiagn Immunother. 32:270-276
- RRID
-
AB_2904437 (BioLegend Cat. No. 600351)
AB_2904437 (BioLegend Cat. No. 600352)
Antigen Details
- Structure
- Aquaporin 4 is a 323 amino acid protein with a molecular mass of 35 kD.
- Distribution
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Tissue distribution: astrocytes in CNS
Cellular distribution: plasma membrane, endosome, and cytoskeleton - Function
- AQP4 facilitates the transport of water across the blood-brain interface.
- Cell Type
- Astrocytes
- Biology Area
- Cell Biology, Neuroscience, Neuroscience Cell Markers
- Antigen References
-
- Salman MM, et al. 2021. Nat Rev Neurosci. 22:650-651
- Tang G and Yang GY. 2016. Int J Mol Sci. 17:1413
- Jarius S and Wildemann B. 2013. Brain Pathol. 23:661-83
- Gene ID
- 361 View all products for this Gene ID
- UniProt
- View information about Aquaporin-4 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All Aquaporin-4 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-Aquaporin-4 | E5206 | IHC-P,ICC,IHC-F,WB,IP |
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