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Purified anti-CD4 Antibody

Pricing & Availability
Clone
W23082E (See other available formats)
Regulatory Status
RUO
Other Names
T4, Leu3, T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
A. W23082E_PURE_CD4_IHC-P_102824
IHC staining with Purified anti-CD4 (clone W23082E) on formalin-fixed paraffin-embedded human tonsil tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., 1X (Cat. No. 928502), the tissue was incubated either without (panel A) or with (panel B) Purified anti-CD4 (clone W23082E) (magenta) and Purified mouse anti-CD8a (clone C8/144B) (Cat. No. 372902) (yellow), followed by incubation with Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405416) and Alexa Fluor® 594 Goat anti-mouse IgG (Cat. No. 405326) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 40X objective and merged. Scale bar: 50 µm
  • A. W23082E_PURE_CD4_IHC-P_102824
    IHC staining with Purified anti-CD4 (clone W23082E) on formalin-fixed paraffin-embedded human tonsil tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., 1X (Cat. No. 928502), the tissue was incubated either without (panel A) or with (panel B) Purified anti-CD4 (clone W23082E) (magenta) and Purified mouse anti-CD8a (clone C8/144B) (Cat. No. 372902) (yellow), followed by incubation with Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405416) and Alexa Fluor® 594 Goat anti-mouse IgG (Cat. No. 405326) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 40X objective and merged. Scale bar: 50 µm
  • B. W23082E_PURE_CD4_WB_102824
    Whole cell extracts (15 µg total protein per lane) from indicated cell lines and/or tissues were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-CD4 (clone W23082E) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
Cat # Size Price Quantity Check Availability Save
649551 25 µg 124€
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649552 100 µg 344€
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Description

CD4 is a single-chain type I transmembrane glycoprotein and a member of the immunoglobulin superfamily. It is expressed on most thymocytes, helper T cells, type II NKT cells, and monocytes/macrophages. CD4 is part of the TCR (T-Cell Receptor)/CD3 complex and binds to the β2 domain of the MHC class II molecule, and participates in TCR signal transduction. CD4 is the receptor of IL-16 and is a coreceptor for the human immunodeficiency virus (HIV) and human herpes virus 7 (HHV-7).

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant fragment of the extracellular domain of human CD4
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
WB - Verified
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg/mL is suggested.  For western blotting, the suggested use of this reagent is 0.1 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

For use in immunohistochemistry on formalin-fixed paraffin-embedded tissue (IHC-P), it is recommended to perform antigen retrieval using Citrate Buffer, 10X (Cat. No. 420902) or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (10X) (Cat. No. 422704). Staining intensity may be stronger using Citrate Buffer.

This product is not recommended for use in immunocytochemistry (ICC) or intracellular flow cytometry (ICFC).

This product does not react with mouse CD4 in Western Blot (WB) or immunohistochemistry on frozen tissues (IHC-F).

Antigen Details

Structure
CD4 is a 458 amino acid protein with a predicted molecular weight of 51.1 kD.
Distribution

Plasma membrane

Function
Co-receptor
Interaction
Part of the TCR/CD3 complex
Ligand/Receptor
Β2 domain from MHC class II, IL-16
Cell Type
Dendritic cells, Macrophages, Monocytes, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References
  1. Zhu J, et al. 2010. Annu Rev Immunol. 28:445.
  2. Vignali DA. 2010. J. Immunol. 184:5933.
  3. Zhou L, et al. 2009. Immunity. 30:646.
  4. Singer A, et al. 2008. Nat Rev Immunol. 8:788.
  5. Zhu J and Paul WE. 2008. Blood. 112:1557.
Gene ID
920 View all products for this Gene ID
UniProt
View information about CD4 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 10.28.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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