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Purified anti-CD66C (CEACAM 6) Antibody (Previously Covance catalog# SIG-3750)

Pricing & Availability
Clone
9A6 (See other available formats)
Regulatory Status
RUO
Other Names
carcinoembryonic antigen-related cell adhesion molecule 6
Previously
Signet Catalog# 750-001
Signet Catalog# 750-005
Covance Catalog# SIG-3750
Isotype
Mouse IgG1, κ
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Product Citations
publications
9A6_Purified_CD66C_Antibody_1_032515
IHC staining of purified anti-CD66C (CEACAM 6) antibody (clone 9A6) on formalin-fixed paraffin-embedded human colon tissue. The tissue was incubated with a 1:40 dilution of the primary antibody for 20 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective.
  • 9A6_Purified_CD66C_Antibody_1_032515
    IHC staining of purified anti-CD66C (CEACAM 6) antibody (clone 9A6) on formalin-fixed paraffin-embedded human colon tissue. The tissue was incubated with a 1:40 dilution of the primary antibody for 20 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective.
  • 9A6_Purified_CD66C_Antibody_3_032515
    Immunofluorescence of THP-1 cells with (A) mouse IgG1, κ isotype control (Negative, Cat. No. 401402) or (B-D) CD66C primary antibody (Clone 9A6). Alexa Fluor® 488 (Green) Goat anti-Mouse IgG (Cat. No. 405319) was used as secondary antibody. Nuclei were counterstained with DAPI (Blue, Cat. No. 422801). The image was captured with a 60X objective using KEYENCE BZ-X700 fluorescence microscope. Exposure time (Seconds) for (A) is 1/12, and (B-D) is 1/15. Concentrations for (A, B) is 1/100, (C) is 1/200 and (D) is 1:500.
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908601 100 µL 203€
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Description

Anti-human CEACAM6 (CEA related cell adhesion molecule 6, non-specific cross reacting antigen (NCA); CD66c)

CEACAM6 is a member of the carcinoembryonic antigen (CEA) gene family. Like other members of the CEA family, CEACAM6, consists of a single N domain, with structural homology to the immunoglobulin variable domains, followed by one immunoglobulin constant like A and B domain.

CEACAM6 encodes a glycosyl phosphatidyl inositol (GPI) linked glycoprotein, which has a molecular weight of 90kDa.

CEACAM6 expression is upregulated in many adenocarcinomas and leukemias. CEACAM6 is expressed in epithelial cells of the fetal and adult gastrointestinal tract, epithelia of glandular tissues, squamous epithelial cell of the tongue, esophagus and cervix as well as on granulocytes.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This antibody was developed against a tumor cell line expressing CEACAM6.
Formulation
Phosphate-buffered solution with BSA + 0.09% NaN3.

Previous lots of this product may have been formulated with 0.1% or 0.05% NaN3 instead of 0.09% NaN3. For further information please contact BioLegend Technical Support or Customer Service.
Preparation
The antibody was purified by affinity chromatography.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
Store between 2°C and 8°C.
Application

IHC-P - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a dilution of 1:40 is suggested. For immunocytochemistry, a concentration range of 1:100 - 1:200 dilution is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Hammarstrom S. 1999. Semin Cancer Biol 9:67-81. (EM)
  2. Hanenberg H, et al. 1994. Leukemia. 12:2127. (ICC)
  3. Daniel S, et al. 1993. Int J Cancer. 55:303. (IHC)
  4. Gottlinger HG, et al. 1988. Cancer Res. 48:2198. (ICC)
Product Citations
  1. Wang C, et al. 2023. Immunity. 576:56. PubMed
RRID
AB_2565088 (BioLegend Cat. No. 908601)

Antigen Details

Structure
Expected MW: 45 kD
Biology Area
Immunology
Molecular Family
CD Molecules
Gene ID
4680 View all products for this Gene ID
UniProt
View information about CD66C on UniProt.org
Go To Top Version: 6    Revision Date: 02.08.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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