- Clone
- 6G12-H11 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- CRISPR associated protein 9, SpyCas9
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
698301 | 25 µg | 81€ | ||||
698302 | 100 µg | 203€ |
Cas9 is an RNA-guided DNA endonuclease enzyme associated with the CRISPR (Clustered Regularly Interspersed Palindromic Repeats) adaptive immunity system in Streptococcus pyogenes, among other bacteria. Cas9 interrogates and cleaves foreign DNA, such as invading bacteriophage DNA or plasmid DNA. Cas9 performs this interrogation by unwinding foreign DNA and checking if it is complementary to the 20 base pair spacer region of the guide RNA. If the DNA substrate is complementary to the guide RNA, Cas9 cleaves the invading DNA. In this sense, the CRISPR-Cas9 mechanism has a number of parallels with the RNA interference (RNAi) mechanism in eukaryotes.
Product DetailsProduct Details
- Verified Reactivity
- S. Pyogenes
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- C-terminal recombinant fragment of S. pyogenes Cas9
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.05 - 0.5 µg per ml (1:1000 - 1:10,000). It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_2715788 (BioLegend Cat. No. 698301)
AB_2715788 (BioLegend Cat. No. 698302)
Antigen Details
- Structure
- The architectures of Cas9 enzymes define nucleic acid binding clefts to form a central channel where DNA substrates are bound. The predicted Mw is 158kDa.
- Distribution
-
S. Pyogenes
- Function
- Part of the CRISPR/Cas9 system of acquired immunity that bacteria have evolved to destroy foreign DNA elements, such as bacteriophages.
- Interaction
- Binds crRNA and tracrRNA
- Antigen References
-
1. Mali P, et al. 2013. Science 339:823.
- Gene ID
- 901176 View all products for this Gene ID
- UniProt
- View information about CRISPR on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All CRISPR (Cas9) Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-CRISPR (Cas9) | 6G12-H11 | WB |
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This data display is provided for general comparisons between formats.
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Purified anti-CRISPR (Cas9)
Total cell lysates (15µg protein) from 293T and 293T transfe...
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