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Purified anti-GABRA2 Antibody

Pricing & Availability
Clone
N399/19 (See other available formats)
Regulatory Status
RUO
Other Names
Gamma-Aminobutyric Acid Type A Receptor Subunit Alpha2, GABRA2, GABA(A) receptor subunit alpha-2, EIEE78, DEE78
Isotype
Mouse IgG1, κ
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Product Citations
publications
N399slash19_PURE_GABRA2_Antibody_102021
IHC staining of anti-GABRA2 antibody (clone N399/19) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928502), the tissue was incubated with 5.0 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin HRP Kit (Multi-Species, DAB, Cat. No. 929501) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 μm.
  • N399slash19_PURE_GABRA2_Antibody_102021
    IHC staining of anti-GABRA2 antibody (clone N399/19) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928502), the tissue was incubated with 5.0 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin HRP Kit (Multi-Species, DAB, Cat. No. 929501) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 μm.
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601552 100 µg 132€
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Description

GABRA2 is a type of alpha subunit which comprises two of the five subunits of the pentameric GABA-A ionotropic receptor. In response to GABA (gamma-aminobutyric acid) binding, the GABA-A receptor permits the passage of negatively-charged chloride ions, inhibiting action potential firing. GABRA2 is present in approximately 35% of all GABA-A receptors and is expressed primarily in the hippocampus and forebrain, where it plays a role in anxiolytic activity. 

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Cytoplasmic domain of recombinant rat GABA(A) Receptor Subunit Alpha-2
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
IHC-F - Reported in the Literature
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Scott H, et al. 2020. Neurobiol. Stress. 13:100281. 
RRID
AB_2904444 (BioLegend Cat. No. 601552)

Antigen Details

Structure
GABRA2 is a 451 amino acid protein with a predicted molecular weight of 51.3 kD.
Distribution

GABRA2 is expressed in GABA-ergic neurons of the central and peripheral nervous system.

Function
GABRA2 is a ligand-gated ion channel for chloride (Cl-) and to a lesser extent bicarbonate (HCO3-) ions.
Ligand/Receptor
GABRA2 is a receptor for GABA (γ-aminobutyric acid)
Antigen References
  1. Sigel E, et al. 2012. J Biol. Chem. 287(48):40224-40231
Gene ID
2555 View all products for this Gene ID
UniProt
View information about GABRA2 on UniProt.org
Go To Top Version: 1    Revision Date: 10.20.2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

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