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Purified anti-Htra2/Omi Antibody

Pricing & Availability
Clone
8G11-4B8-4F10-G5 (See other available formats)
Regulatory Status
RUO
Other Names
HTRA Serine Petidase 2, OMI, PRSS25, PARK13, High Temperature Requirement Protein A2, Serine Protease HTRA2 Mitochondrial, OMI Stress-Regulated Endoprotease, Serine Proteinase OMI, MGCA8
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
8G11-4B8-4F10-G5_PURE_Htra2slashOmi_Antibody_IP_012521.png
Whole cell extracts (250 µg total protein) prepared from HeLa cells were immunoprecipitated overnight with 2.0 µg of purified mouse IgG1, k isotype ctrl antibody (Cat. No. 400102) or purified anti-HTRA2 antibody (clone 8G11-4B8-4F10-G5). The resulting IP fractions and whole cell extract input (6%) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane and probed with a control antibody against a separate epitope of HTRA2. Lane M: Molecular weight marker.
  • 8G11-4B8-4F10-G5_PURE_Htra2slashOmi_Antibody_IP_012521.png
    Whole cell extracts (250 µg total protein) prepared from HeLa cells were immunoprecipitated overnight with 2.0 µg of purified mouse IgG1, k isotype ctrl antibody (Cat. No. 400102) or purified anti-HTRA2 antibody (clone 8G11-4B8-4F10-G5). The resulting IP fractions and whole cell extract input (6%) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane and probed with a control antibody against a separate epitope of HTRA2. Lane M: Molecular weight marker.
  • 8G11-4B8-4F10-G5_PURE_Htra2slashOmi_Antibody_WB_012521.png
    Whole cell extracts (15 µg total protein) from K562, MOLT-4, Hep G2, and HeLa (positive control) lysates and mouse-brain lysates were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 μg/mL (1:500 dilution) purified anti-HTRA2 antibody (clone 8G11-4B8-4F10-G5) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker.
Cat # Size Price Quantity Check Availability Save
944501 25 µg 95€
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944502 100 µg 235€
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Description

Htra2/Omi (high temperature requirement 2) is a mitochondrial serine protease and member of the high-temperature requirement A family of oligomeric serine proteases that functions in the regulation of apoptosis, mitochondrial homeostasis, and protein quality control. Under normal conditions Htra2/Omi is localized to the mitochondria where it serves as a chaperone protein. Under stress conditions Htra2/Omi is released to the cytosol where it mediates apoptosis via proteolytic activity and disruption of caspase-IAP (inhibitors of apoptosis proteins) activity as well as through caspase-independent pathways. Loss or disruption of Htra2/Omi function results in mitochondrial defects and the aggregation of unfolded or misfolded proteins and has been implicated in various neurodegenerative disorders such as Parkinson’s disease, thus making Htra2/Omi an important potential therapeutic target in the treatment of such disorders. Likewise, due to its role as a regulator of cell death, Htra2/Omi may also be an important therapeutic target in the treatment of systemic sepsis and the prevention of Sepsis-Associated Encephalopathy (SAE) as well as a potential oncogenic target and modulator of chemotherapy-induced cytotoxicity.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
recombinant human Htra2 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IP - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. For immunoprecipitation, the suggested use of this reagent is 2.0 µg/test. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone was tested for ICC on HeLa cells using three different fixation/permeabilization methods: methanol-only, PFA+ methanol, and PFA+ Triton X-100. Staining was only observed with PFA+ Triton X-100 permeabilization and even this was quite faint and only detected at high exposures.

This clone is weakly reactive with mouse Htra2 by western blot.

RRID
AB_2890873 (BioLegend Cat. No. 944501)
AB_2890873 (BioLegend Cat. No. 944502)

Antigen Details

Structure
HTRA2 is a 458 amino acid protein with an expected molecular weight of 38 to 49 kD.
Distribution

Ubiquitous/ Mitochondria

Function
Protease, Hydrolase, Apoptosis
Antigen References
1. Chen S, et al. 2018. Genes. 9:355.
2. Chien J, et al. 2009. Curr. Cancer Drug Targets. 9:451.
3. Hu Y, et al. 2019. Front. Mol. Neurosci. 12:48.
4. Zurawa-Janicka D, et al. 2013. Cell Stress Chaperones. 18:35.
Gene ID
27429 View all products for this Gene ID
UniProt
View information about Htra2/Omi on UniProt.org
Go To Top Version: 1    Revision Date: 01.25.2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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