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Purified anti-human CCRL2 Antibody

Pricing & Availability
Clone
K097F7 (See other available formats)
Regulatory Status
RUO
Other Names
CCR11, CKRX, CRAM, HCR
Isotype
Mouse IgG2a, κ
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Product Citations
publications
K097F7_Purified_CCRL2_Antibody_FC_051413
Human peripheral blood monocytes were stained with purified CCRL2 (clone K097F7, filled histogram) or purified mouse IgG2a, κ isotype control (open histogram), followed by anti-mouse IgG PE.
  • K097F7_Purified_CCRL2_Antibody_FC_051413
    Human peripheral blood monocytes were stained with purified CCRL2 (clone K097F7, filled histogram) or purified mouse IgG2a, κ isotype control (open histogram), followed by anti-mouse IgG PE.
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358302 100 µg 156€
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Description

CCRL2 (C-C chemokine receptor-like 2) is a seven transmembrane, G-protein coupled receptor with two isoforms generated from alternative splicing. CCRL2 is expressed by the majority of cell types of hematopoietic origin, including monocytes, macrophages, neutrophils, lymphocytes, and NK cells. In some cell types, its expression is upregulated by inflammatory stimuli. CCRL2 is important for the development of Th2 responses and may act as a decoy receptor capturing and endocyting its ligands CCL5 and CCL19; however, it has been found that CCRL2 captures and retains chemerin/RARRES2 on its cell surface, presenting it to neighboring cells.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Expression vector containing the full sequence of CCRL2
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Migeotte I, et al. 2002. Eur. J. Immunol. 32:494. (FC)
RRID
AB_2562116 (BioLegend Cat. No. 358302)

Antigen Details

Structure
Seven transmembrane, G-protein coupled receptor, 2 isoforms
Distribution

Majority of cell types of hematopoietic origin, including monocytes, macrophages, neutrophils, lymphocytes, and NK cells; upregulated after inflammatory stimuli

Function
May act as a decoy receptor capturing and endocyting its ligands; captures chemerin/RARRES2 on the cell surface to present it to neighboring cells
Ligand/Receptor
CCL5, CCL19, chemerin/RARRES2
Cell Type
B cells, Dendritic cells, Neutrophils
Biology Area
Cell Biology, Immunology
Molecular Family
Cytokine/Chemokine Receptors, GPCR
Antigen References

1. Yin F, et al. 2012. Biochem. Biophys. Res. Commun. 429:168.
2. An P, et al. 2011. PLoS Genet. 10:e1002328.
3. Yoshimura T and Oppenheim JJ. 2011. Exp. Cell. Res. 317:674.

Gene ID
9034 View all products for this Gene ID
UniProt
View information about CCRL2 on UniProt.org

Related FAQs

Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?

Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.

Go To Top Version: 1    Revision Date: 05.22.2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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