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Purified anti-human CD144 (VE-Cadherin) Antibody

Pricing & Availability
Clone
BV9 (See other available formats)
Regulatory Status
RUO
Other Names
VE-Cadherin, cadherin-5, CDH5
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
BV9_PE_052710
Human umbilical vein endothelial cells, HUVEC, stained with purified BV9 conjugated with PE
  • BV9_PE_052710
    Human umbilical vein endothelial cells, HUVEC, stained with purified BV9 conjugated with PE
  • BV9_Purified_CD144_Antibody_ICC_011821
    HUVEC cells were fixed with 1% paraformaldehyde (PFA) and then stained with 10 µg/ml of purified CD144 (VE-Cadherin) (clone BV9) at 4°C overnight, followed by 2.5 µg/ml of Alexa Fluor® 555 anti-mouse IgG (yellow) for 2 hours at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured by 20X objective.
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348501 25 µg 76€
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348502 100 µg 169€
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Description

CD144, also known as VE-cadherin and cadherin-5, is a 140 kD glycoprotein which is composed of five extracellular cadherin repeats and a highly conserved cytoplasmic tail region. It is a calcium-dependent transmembrane cell-cell adhesion molecule localized at the intercellular boundaries of endothelial cells, hematopoietic stem cells, and perineurial cells. It functions as a classic cadherin by mediating homophilic adhesion and functions as a plasma membrane attachment site for the cytoskeleton. CD144 is thought to play a role in vascular development, permeability, and remodeling.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
ICC - Verified
WB, IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. For immunocytochemistry, a concentration range of 5.0 - 10 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

It is also recommended using EDTA-based solutions for dissociating attachment-dependent cell lines.

Application Notes

Clone BV9 has been shown to block VE-cadherin, causing a redistribution of VE-cadherin away from intracellular junctions.6 This clone binds to EC3-EC4 region in the extracellular domain of human VE-cadherin.7 Additional reported applications (for the relevant formats) include: Western Blotting1,2, immunofluorescence microscopy1,3, immunoprecipitation1,4, blocking angiogenesis in vitro4,5, inhibiting VE-cadherin reorganization4, and inducing endothelial cell apoptosis4.

Application References

(PubMed link indicates BioLegend citation)
  1. Almagro S, et al. 2010. Mol. Cell Biol. 30:1703. (WB, IF, IP)
  2. Zhang F, et al. 2004. J. Biol. Chem. 279:11760. (WB)
  3. Iurlaro M, et al. 2004. Am. J. Pathol. 165:181. (IF)
  4. Corada M, et al. 2001. Blood 97:1679. (IP, Block)
  5. Kooistra M, et al. 2005. FEBS 579:4966. (Block)
  6. Corada M, et al. 2001. Blood 97:1679. (Block)
  7. Bouillet L, et al. 2013. Laboratory Investigation 93:1194-11202.
Product Citations
  1. Ghoroghi S, et al. 2021. Elife. 10:. PubMed
  2. Barzilai S, et al. 2017. Cell Rep. 18(3):685-699. PubMed
  3. Piao W, et al. 2020. Cell Reports. 30(4):1052-1062.e5.. PubMed
  4. Bashth OS, et al. 2020. Biomater Sci. 4.128472222. PubMed
  5. Boulanger MD, et al. 2022. PLoS One. 17:e0269316. PubMed
  6. Molina SG, et al. 2020. Stem Cell Res. 49:102015. PubMed
  7. Kondo N, et al. 2020. Cancers (Basel). 12:00. PubMed
  8. Liu X, et al. 2019. J Am Heart Assoc. 8:e013323. PubMed
RRID
AB_10574464 (BioLegend Cat. No. 348501)
AB_10574464 (BioLegend Cat. No. 348502)

Antigen Details

Structure
Member of the cadherin family; calcium-dependent transmembrane cell-cell adhesion glycoprotein composed of five extracellular cadherin repeats and a highly conserved cytoplasmic tail region
Distribution

Endothelial cells, hematopoietic stem cells, perineurial cells

Function
Mediates calcium-dependent homophilic cell adhesion, plays fundamental roles in microvascular permeability and in the morphogenic and proliferative events associated with angiogenesis
Ligand/Receptor
VE-cadherin
Cell Type
Endothelial cells, Hematopoietic stem and progenitors, Mesenchymal Stem Cells
Biology Area
Angiogenesis, Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules, Protein Kinases/Phosphatase
Antigen References

1. Taddei A, et al. 2008. Nat. Cell Biol. 10:923.
2. Gavard J, et al. 2006. Nat. Cell Biol. 8:1223.
3. Kim I, et al. 2005. Blood 106:903.
4. Suzuki S, et al. 1991. Cell Regul. 2:261.

Gene ID
1003 View all products for this Gene ID
UniProt
View information about CD144 on UniProt.org
Go To Top Version: 5    Revision Date: 01.18.2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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