- Clone
- 24-31 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- CD40L, gp39, TRAP, T-BAM, TNFSF5
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
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310835 | 100 µg | 184€ |
CD154 (CD40 ligand) is also known as CD40L, gp39, TRAP and T-BAM. CD40 ligand is a 32-39 kD type II transmembrane glycoprotein. It is a member of the TNF superfamily and is expressed on activated T cells. It has been reported to be important for B cell costimulation following binding of its receptor, CD40. Additionally, binding of CD40L to CD40 on B cells promotes the secretion of immunoglobulin and Ig isotype switching. CD40L is also involved in the regulation of cytokine production by T cells.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Reported Reactivity
- Cynomolgus, Rhesus
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1.0 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
CyTOF® - Verified - Recommended Usage
-
This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.
- Application Notes
-
Additional reported applications (for the relevant formats) include: immunofluorescence microscopy1,3 and blocking of T cell-dependent B cell differentiation1,2,4,5. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 310812). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 310828) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
- Additional Product Notes
-
Maxpar® is a registered trademark of Standard BioTools Inc.
-
Application References
(PubMed link indicates BioLegend citation) -
- Brams P, et al. 2001. Int. Immunopharmacol. 1:277. (Block, IF)
- Rushworth SA, et al. 2002. Transplantation 73:635. (Block)
- Berner B, et al. 2000. Ann. Rheum. Dis. 59:190. (IF)
- Nordström T, et al. 2006. J. Leukocyte Biol. 79:319. (Block)
- Zhang AL, et al.2007. Blood doi:10.1182/blood-2007-02-076364. (Block) PubMed
- Kuchen S, et al. 2007. J. Immunol. 179:5886.
- Matus-Nicodermos R, et al. 2011. J. Immunol. 186:2164. PubMed
- Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
- Product Citations
-
- RRID
-
AB_2563722 (BioLegend Cat. No. 310835)
Antigen Details
- Structure
- TNF superfamily, type II transmembrane glycoprotein, cleaved as soluble CD40L, 32-39 kD
- Distribution
-
Activated T cells
- Function
- B cell costimulation
- Ligand/Receptor
- CD40
- Cell Type
- T cells, Tregs
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Najafian N, et al. 2003. Expert Opin. Biol. Ther. 3:227.
2. Racke M, et al. 2002. Expert Opin. Ther. Targets. 6:275.
3. Ford G, et al. 1999. J. Immunol. 162:4037. - Gene ID
- 959 View all products for this Gene ID
- UniProt
- View information about CD154 on UniProt.org
Related FAQs
- Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?
-
We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm®. Please contact Fluidigm® directly for additional data and further details.
- Can I use Maxpar® Ready format clones for flow cytometry staining?
-
We have not tested the Maxpar® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.
- I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.
-
We only supply the antibody and not test that in house. Please contact Fluidigm® directly for troubleshooting advice: http://techsupport.fluidigm.com/
- Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?
-
The Maxpar® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.
Other Formats
View All CD154 Reagents Request Custom ConjugationCompare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD154
PMA + ionomycin-stimulated human PBMCs (5 hours) stained wit... -
FITC anti-human CD154
TPA+ ionomycin-stimulated human PBMCs (6 hours) stained with... -
PE anti-human CD154
PMA+ionomycin-stimulated human PBMCs (6 hours) stained with ... -
PE/Cyanine5 anti-human CD154
TPA+ ionomycin-stimulated human PBMCs (6 hours) stained with... -
APC anti-human CD154
TPA+ ionomycin-stimulated human PBMCs (5 hours) stained with... -
Biotin anti-human CD154
TPA+ ionomycin-stimulated human PBMCs (5 hours) stained with... -
Alexa Fluor® 488 anti-human CD154
TPA+ ionomycin-stimulated human PBMCs (5 hours) stained with... -
Alexa Fluor® 647 anti-human CD154
TPA+ ionomycin-stimulated human PBMCs (5 hours) stained with... -
Pacific Blue™ anti-human CD154
PMA+ionomycin-stimulated human PBMCs (6 hours) stained with ... -
Brilliant Violet 785™ anti-human CD154
PMA + ionomycin-stimulated (6 hours) human peripheral blood ... -
APC/Cyanine7 anti-human CD154
TPA+ ionomycin-stimulated human PBMCs (5 hours) stained with... -
Brilliant Violet 421™ anti-human CD154
PMA+ionomycin-stimulated human peripheral blood lymphocytes ... -
Brilliant Violet 605™ anti-human CD154
6-hour PMA+ionomycin-stimulated human peripheral blood lymph... -
Ultra-LEAF™ Purified anti-human CD154
PMA+ionomycin-stimulated human PBMCs (6 hours) stained with ... -
Brilliant Violet 510™ anti-human CD154
PMA+ionomycin-stimulated (6 hours) human peripheral blood ly... -
PE/Cyanine7 anti-human CD154
PMA+ionomycin-stimulated (6 hours) human peripheral blood ly... -
PerCP/Cyanine5.5 anti-human CD154
PMA+ ionomycin-stimulated (6 hours) human peripheral blood l... -
Brilliant Violet 711™ anti-human CD154
PMA+ionomycin-stimulated (6 hours) human peripheral blood ly... -
Purified anti-human CD154 (Maxpar® Ready)
Human PBMCs were incubated for 6 hours in media alone (top) ... -
PE/Dazzle™ 594 anti-human CD154
PMA+ionomycin (six hours) stimulated human peripheral blood ... -
TotalSeq™-A0032 anti-human CD154
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Alexa Fluor® 700 anti-human CD154
PMA+ionomycin-stimulated (6 hours) human peripheral blood ly... -
APC/Fire™ 750 anti-human CD154
PMA+ionomycin-stimulated (6 hours) human peripheral blood ly... -
TotalSeq™-C0032 anti-human CD154
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TotalSeq™-B0032 anti-human CD154
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TotalSeq™-D0032 anti-human CD154
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PE/Fire™ 640 anti-human CD154
Human peripheral blood lymphocytes were stimulated with PMA+... -
PE/Fire™ 810 anti-human CD154
Human peripheral blood lymphocytes were stimulated with PMA+... -
PerCP/Fire™ 780 anti-human CD154
Human peripheral blood lymphocytes were stimulated with PMA+... -
PE/Fire™ 700 anti-human CD154
Human peripheral blood lymphocytes were stimulated with Cell... -
Spark Red™ 718 anti-human CD154 (Flexi-Fluor™)
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Brilliant Violet 650™ anti-human CD154
Human peripheral blood mononuclear cells were stimulated wit... -
Spark Blue™ 574 anti-human CD154 (Flexi-Fluor™)
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Spark Blue™ 550 anti-human CD154 (Flexi-Fluor™)
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PE/Cyanine7 anti-human CD154
Typical results from PMA + Ionomycin without Brefeldin A sti...
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