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Purified anti-human CD16 Recombinant Antibody

Pricing & Availability
Clone
B73.1.Rec (See other available formats)
Regulatory Status
RUO
Other Names
FcγRIII, Fc gamma receptor, Fc gamma receptor 3
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
B731-Rec_PURE_CD16_FC_122724
Human peripheral blood lymphocytes were stained with purified anti-human CD16 recombinant (clone B73.1.Rec) (left), or purified mouse IgG1, κ isotype control (right) followed by anti-mouse IgG PE. Cells were then co-stained with anti-human CD56 (clone HCD56) Brilliant Violet 421™.
  • B731-Rec_PURE_CD16_FC_122724
    Human peripheral blood lymphocytes were stained with purified anti-human CD16 recombinant (clone B73.1.Rec) (left), or purified mouse IgG1, κ isotype control (right) followed by anti-mouse IgG PE. Cells were then co-stained with anti-human CD56 (clone HCD56) Brilliant Violet 421™.
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318015 100 µg 100€
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Description

CD16 is known as low affinity IgG receptor III (FcγRIII). It is expressed as two distinct forms (CD16a and CD16b). CD16a (FcγRIIIA) is a 50-65 kD polypeptide-anchored transmembrane protein. It is expressed on the surface of NK cells, activated monocytes, macrophages, and placental trophoblasts in humans. CD16b (FcγRIIIB) is a 48 kD glycosylphosphatidylinositol (GPI)-anchored protein. Its extracellular domain is over 95% homologous to that of CD16a, and it is expressed specifically on neutrophils. CD16 binds aggregated IgG or IgG-antigen complex which functions in NK cell activation, phagocytosis, and antibody-dependent cell-mediated cytotoxicity (ADCC).

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Recombinant
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Antigen Details

Structure
Ig superfamily, transmembrane form (50-65 kD) or GPI-linked form (48 kD)
Distribution

NK cells, activated monocytes, macrophages, neutrophils and a subset of T cells

Function
Low affinity IgG Fc receptor, phagocytosis, ADCC
Interaction
CD16 binds aggregated IgG or IgG-antigen complex which functions in NK cell activation, phagocytosis, and antibody-dependent cell-mediated cytotoxicity (ADCC)
Ligand/Receptor
IgG Fc receptor III (FcγRIII)
Cell Type
Macrophages, Monocytes, Neutrophils, NK cells, T cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, Fc Receptors
Antigen References
  1. Rataj F, et al. 2019. Br J Cancer. 120:79-87.
  2. Kaufmann M, et al. 2021. Med. 2:296-312.
  3. Sigal N, et al. 2022. Methods Mol Biol. 2543:113-128. 
  4. Cheung P, et al. 2018. Cell. 173:1385. 
  5. Prodjinotho UF, et al. 2017. PLoS Negl Trop Dis. 11:e0005777. 
  6. Roussel M, et al. 2021. Cell Reports Medicine. 2:100291. 
  7. Waugh KA, et al. 2020. Cell Reports. 29:1893-1908.e4.
Gene ID
2214 View all products for this Gene ID
UniProt
View information about CD16 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 12.30.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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