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Purified anti-human CD194 (CCR4) Recombinant Antibody

Pricing & Availability
Clone
QA21A43 (See other available formats)
Regulatory Status
RUO
Other Names
CKR4, K5-5, CMKBR4, ChemR13, CC-CKR-4, MGC88293, HGCN:14099
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
QA21A43_PURE_CD194_Antibody_1_070523
Human peripheral blood lymphocytes were stained with purified anti-human CD194 (CCR4) recombinant (clone QA21A43) (left) or purified mouse IgG1, κ isotype control (right) followed by goat anti-mouse IgG PE. Cells were costained with anti-human CD3 FITC.
  • QA21A43_PURE_CD194_Antibody_1_070523
    Human peripheral blood lymphocytes were stained with purified anti-human CD194 (CCR4) recombinant (clone QA21A43) (left) or purified mouse IgG1, κ isotype control (right) followed by goat anti-mouse IgG PE. Cells were costained with anti-human CD3 FITC.
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388002 100 µg 188€
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Description

CD194, also known as CCR4, is a CC chemokine receptor. It binds CCL17 and CCL22 and is expressed on a subset of T and B cells, basophils, monocytes, and NK cells. Human Th2 cells are characterized by the expression of CCR4 and CCR8, and these receptors are regulated differently during Th2 development. Human peripheral blood Tregs can be divided into two distinct populations based on the expression of CCR4. Freshly isolated Tregs express CCR4 and presumably represent memory-type Tregs, and CCR4- Tregs require CD3-mediated activation to acquire a regulatory activity. Depletion of CCR4+ T cells leads to Th1-type polarization of CD4+ T cells and augmentation of CD8+ T cell responses to tumor antigens. CCR4 and its ligands are important for the recruitment of memory T cells into the skin in various cutaneous immune diseases.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Recombinant
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone QA21A43 can completely block the binding of clone L291H4 on target cells.

RRID
AB_3068206 (BioLegend Cat. No. 388002)

Antigen Details

Structure
GPCR, seven transmembrane receptor
Distribution

Subsets of T and B cells, basophils, monocytes, NK cells

Function
Migration of inflammatory and regulatory cells to the target tissues
Ligand/Receptor
CCL17 and CCL22
Cell Type
B cells, Basophils, Embryonic Stem Cells, Monocytes, NK cells, T cells, Tregs
Biology Area
Immunology, Innate Immunity, Stem Cells
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References
  1. Katschke KJ, et al. 2001. Arthritis Rheum. 44:1022-32.
  2. Colantonio L, et al. 2002. Eur J Immunol. 32:1264-73.
  3. Jakubzick C, et al. 2004. Am J Pathol. 165:1211-21.
  4. Morimoto Y, et al. 2005. J Leukoc Biol. 78:753-61.
  5. Baatar D, et al. 2007. J Immunol. 178:4891-900.
  6. Kusumoto M, et al. 2007. J Interferon Cytokine Res. 27:901-10.
Gene ID
1233 View all products for this Gene ID
UniProt
View information about CD194 on UniProt.org
Go To Top Version: 1    Revision Date: 07.05.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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