- Clone
- HIB22 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- V CD22.14
- Other Names
- BL-CAM, Siglec-2, Lyb8
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
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302511 | 100 µg | 113€ |
CD22 is a 130 kD type I transmembrane glycoprotein also known as Siglec-2 and BL-CAM. It is a member of the immunoglobulin superfamily (sialoadhesion subgroup). CD22 is expressed in the cytoplasm of pro-B and pre-B cells, and on the surface of mature B and activated B cells, but not on plasma cells. CD22 is present in the B cell receptor complex and associates with SHP-1, Syk, Lck, Lyn, and phospholipase Cγ1. A primary function of CD22 is thought to be in limiting antigen receptor signaling by modulating B cell activation threshold. CD22 has been shown to bind to CD45RO and CD75, although the natural ligands for this molecule remain controversial.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Reported Reactivity
- Chimpanzee
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1.0 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
CyTOF® - Verified - Recommended Usage
-
This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.
- Application Notes
-
Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections.
- Additional Product Notes
-
Maxpar® is a registered trademark of Standard BioTools Inc.
-
Application References
(PubMed link indicates BioLegend citation) -
- Schlossman S, et al. Eds. 1995. Leukocyte Typing V:White Cell Differentiation Antigens. Oxford University Press. New York.
- Clark E. 1993. J. Immunol.. 150:4715.
- Shan D and O. Press. 1995. J. Immunol.. 154:4466.
- Product Citations
-
- RRID
-
AB_2563730 (BioLegend Cat. No. 302511)
Antigen Details
- Structure
- Ig superfamily, a type I glycosylated integral transmembrane protein, 120-130 kD
- Distribution
-
B cells
- Function
- Adhesion, B-monocyte and B-T interactions
- Ligand/Receptor
- CD45RO, CD75
- Cell Type
- B cells
- Biology Area
- Cell Adhesion, Cell Biology, Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules, Siglec Molecules
- Antigen References
-
1. Clark E. 1993. J. Immunol. 150:4715.
2. Shan D, et al. 1995. J. Immunol. 154:4466. - Gene ID
- 933 View all products for this Gene ID
- UniProt
- View information about CD22 on UniProt.org
Related FAQs
- Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?
-
We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm®. Please contact Fluidigm® directly for additional data and further details.
- Can I use Maxpar® Ready format clones for flow cytometry staining?
-
We have not tested the Maxpar® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.
- I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.
-
We only supply the antibody and not test that in house. Please contact Fluidigm® directly for troubleshooting advice: http://techsupport.fluidigm.com/
- Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?
-
The Maxpar® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.
Other Formats
View All CD22 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
FITC anti-human CD22 | HIB22 | FC |
PE anti-human CD22 | HIB22 | FC |
PE/Cyanine5 anti-human CD22 | HIB22 | FC |
Purified anti-human CD22 | HIB22 | FC,IHC-F |
APC anti-human CD22 | HIB22 | FC |
PE/Cyanine7 anti-human CD22 | HIB22 | FC |
PerCP/Cyanine5.5 anti-human CD22 | HIB22 | FC |
Purified anti-human CD22 (Maxpar® Ready) | HIB22 | FC,CyTOF® |
Alexa Fluor® 647 anti-human CD22 | HIB22 | FC |
APC/Fire™ 750 anti-human CD22 | HIB22 | FC |
Brilliant Violet 421™ anti-human CD22 | HIB22 | FC |
Brilliant Violet 510™ anti-human CD22 | HIB22 | FC |
APC/Cyanine7 anti-human CD22 | HIB22 | FC |
PE/Dazzle™ 594 anti-human CD22 | HIB22 | FC |
PerCP/Cyanine5.5 anti-human CD22 | HIB22 | FC |
Spark Violet™ 423 anti-human CD22 | HIB22 | FC |
GMP PerCP/Cyanine5.5 anti-human CD22 | HIB22 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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FITC anti-human CD22
Human peripheral blood lymphocytes stained HIB22 FITC -
PE anti-human CD22
Human peripheral blood lymphocytes stained with HIB22 PE -
PE/Cyanine5 anti-human CD22
Human peripheral blood lymphocytes stained HIB22 PE/Cyanine5 -
Purified anti-human CD22
Human peripheral blood lymphocytes stained with purified HIB... -
APC anti-human CD22
Human peripheral blood lymphocytes stained with HIB22 APC -
PE/Cyanine7 anti-human CD22
Human peripheral blood lymphocytes were stained with CD22 (c... -
PerCP/Cyanine5.5 anti-human CD22
Human peripheral blood lymphocytes were stained with CD19 AP... -
Purified anti-human CD22 (Maxpar® Ready)
Human PBMCs stained with 154Sm-anti-CD45 (HI30) and 159Tb-an... -
Alexa Fluor® 647 anti-human CD22
Human peripheral blood lymphocytes were stained with CD22 (c... -
APC/Fire™ 750 anti-human CD22
Human peripheral blood lymphocytes were stained with CD22 (c... -
Brilliant Violet 421™ anti-human CD22
Human peripheral blood lymphocytes were stained with CD22 (c... -
Brilliant Violet 510™ anti-human CD22
Human peripheral blood lymphocytes were stained with CD22 (c... -
APC/Cyanine7 anti-human CD22
Human peripheral blood lymphocytes were stained with CD19 FI... -
PE/Dazzle™ 594 anti-human CD22
Human peripheral blood lymphocytes were stained with CD19 Br... -
PerCP/Cyanine5.5 anti-human CD22
Typical results from human peripheral blood lymphocytes stai... -
Spark Violet™ 423 anti-human CD22
Human peripheral blood lymphocytes were stained with anti-hu... -
GMP PerCP/Cyanine5.5 anti-human CD22
Typical results from human peripheral blood lymphocytes stai...
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