- Clone
- WM59 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- V P025
- Other Names
- PECAM-1, EndoCAM
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
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303127 | 100 µg | 104€ |
CD31 is a 130-140 kD type I transmembrane glycoprotein also known as platelet endothelial cell adhesion molecule-1 (PECAM-1) or Endocam. It is expressed on monocytes, platelets, granulocytes, endothelial cells and lymphocyte subsets. CD31 has been reported to bind CD38 and be involved in wound healing, angiogenesis, and cellular migration in an inflammatory situation.
Product DetailsProduct Details
- Verified Reactivity
- Human, Cynomolgus, Rhesus
- Reported Reactivity
- African Green, Baboon
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1.0 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
CyTOF® - Verified - Recommended Usage
-
This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.
- Application Notes
-
Clone WM59 has been reported to recognize the D2 extracellular portion of CD31.
Additional reported applications (for the relevant formats) include: immunofluorescence microscopy2, immunohistochemical staining of acetone-fixed frozen tissue sections8, blocking of platelet aggregation3, and spatial biology (IBEX)11,12. Clone WM59 is not recommended for immunohistochemical staining of formalin-fixed paraffin-embedded sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 303143 & 303144).
The purified WM59 antibody is useful as a capture antibody for a sandwich ELISA assay, when used in conjunction with biotin anti-human CD31 antibody (Cat. No. 536604) antibody as the detection antibody. - Additional Product Notes
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Maxpar® is a registered trademark of Standard BioTools Inc.
-
Application References
(PubMed link indicates BioLegend citation) -
- Schlossman S, et al. Eds. 1995. Leucocyte Typing V Oxford University Press. New York.
- Muczynski KA, et al. 2003. J. Am. Soc. Nephrol. 14:1336. (IF)
- Wu XW, et al. 1997. Arterioscl. Throm. Vas. 17:3154. (Block)
- Nagano M, et al. 2007. Blood 110:151. (FC) PubMed
- MacFadyen JR, et al. 2005. FEBS Lett. 579:2569. PubMed
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Sestak K, et al. 2007. Vet. Immunol. Immunopathol. 119:21.
- Wicki A, et al. 2012. Clin. Cancer Res. 18:454. (FC, IHC) PubMed
- Oeztuerk-Winder F, et al. 2012. EMBO J. 31:3431. (FC) PubMed
- Bushway ME, et al. 2014. Biol Reprod. 90(5): 110 (IF) PubMed
- Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
- Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
- Product Citations
-
- RRID
-
AB_2563740 (BioLegend Cat. No. 303127)
Antigen Details
- Structure
- Ig superfamily, type I transmembrane glycoprotein, 130-140 kD
- Distribution
-
Monocytes, platelets, granulocytes, endothelial cells, lymphocyte subset
- Function
- Cell adhesion, signal transduction
- Ligand/Receptor
- CD38
- Cell Type
- Endothelial cells, Granulocytes, Lymphocytes, Monocytes, Neutrophils, Platelets
- Biology Area
- Angiogenesis, Cell Adhesion, Cell Biology, Immunology, Neuroinflammation, Neuroscience
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
- DeLisser H, et al. 1994. Immunol. Today 15:490.
- Newman P, 1997. J. Clin. Invest. 99:3.
- Fawcett J, et al. 1995. J. Cell Biol. 128:1229.
- Gene ID
- 5175 View all products for this Gene ID
- UniProt
- View information about CD31 on UniProt.org
Related FAQs
- Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?
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We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm®. Please contact Fluidigm® directly for additional data and further details.
- Can I use Maxpar® Ready format clones for flow cytometry staining?
-
We have not tested the Maxpar® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.
- I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.
-
We only supply the antibody and not test that in house. Please contact Fluidigm® directly for troubleshooting advice: http://techsupport.fluidigm.com/
- Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?
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The Maxpar® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.
Other Formats
View All CD31 Reagents Request Custom ConjugationCompare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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FITC anti-human CD31
Human peripheral blood lymphocytes, monocytes and granulocyt... -
PE anti-human CD31
Human peripheral granulocytes were stained with CD31 (clone ... Confocal image of human spleen sample acquired using the IBE... Confocal image of human lymph node sample acquired using the... Confocal image of human jejunum sample acquired using the IB... -
Purified anti-human CD31
Human peripheral blood lymphocytes, monocytes and granulocyt... HUVEC cells were fixed with 1% paraformaldehyde (PFA) and bl... -
Alexa Fluor® 488 anti-human CD31
Human peripheral blood granulocytes were stained with CD31 (... -
Alexa Fluor® 647 anti-human CD31
Human peripheral blood lymphocytes, monocytes and granulocyt... -
Pacific Blue™ anti-human CD31
Human peripheral blood monocytes were stained with CD31 (clo... -
APC anti-human CD31
Human peripheral blood lymphocytes, monocytes and granulocyt... -
PE/Cyanine7 anti-human CD31
Human peripheral blood lymphocytes, monocytes and granulocyt... -
APC/Cyanine7 anti-human CD31
Human peripheral blood granulocytes were stained with CD31 (... -
Brilliant Violet 605™ anti-human CD31
Human peripheral blood granulocytes were stained with anti-h... -
Brilliant Violet 421™ anti-human CD31
Human peripheral blood granulocytes were stained with CD31 (... -
Purified anti-human CD31 (Maxpar® Ready)
Human PBMCs stained with 154Sm-anti-CD45 (HI30) and 145Nd-an... -
Alexa Fluor® 594 anti-human CD31
HUVEC human endothelial cells were fixed with 1% paraformald... -
PE/Dazzle™ 594 anti-human CD31
Human peripheral blood granulocytes were stained with CD31 (... -
PerCP/Cyanine5.5 anti-human CD31
Human peripheral blood granulocytes were stained with CD31 (... -
Alexa Fluor® 700 anti-human CD31
Human peripheral blood granulocytes were stained with CD31 (... Confocal image of human lymph node sample acquired using the... -
Brilliant Violet 711™ anti-human CD31
Human peripheral blood granulocytes were stained with CD31 (... -
TotalSeq™-A0124 anti-human CD31
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TotalSeq™-C0124 anti-human CD31
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APC/Fire™ 750 anti-human CD31
Human peripheral blood granulocytes were stained with CD31 (... -
Ultra-LEAF™ Purified anti-human CD31
Human peripheral blood lymphocytes, monocytes and granulocyt... -
TotalSeq™-B0124 anti-human CD31
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Brilliant Violet 785™ anti-human CD31
Human peripheral blood granulocytes were stained with anti-h... -
PE/Cyanine5 anti-human CD31
Human peripheral blood granulocytes were stained with PE/Cya... -
TotalSeq™-D0124 anti-human CD31
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PE/Fire™ 700 anti-human CD31
Human peripheral blood granulocytes were stained with anti-h... -
Spark YG™ 581 anti-human CD31
Human peripheral blood lymphocytes were stained with anti-hu... -
Brilliant Violet 510™ anti-human CD31
Human peripheral blood lymphocytes were surface stained with... -
Spark Red™ 718 anti-human CD31 (Flexi-Fluor™)
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FITC anti-human CD31
Typical results from human peripheral granulocytes stained e...
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