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Purified anti-human eIF5A Antibody

Pricing & Availability
Clone
W19057C (See other available formats)
Regulatory Status
RUO
Other Names
EIF5A1, EIF-4D, Eukaryotic Initiation Factor 5A Isoform 1, Eukaryotic Initiation Factor 5A
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W19057C_PURE_eIF5A_Antibody_04012021.png
Whole cell extracts (15 µg total protein) from the indicated cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.25 µg/mL purified anti-eIF5A antibody (clone W19057C) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 649203) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular weight marker.
  • W19057C_PURE_eIF5A_Antibody_04012021.png
    Whole cell extracts (15 µg total protein) from the indicated cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.25 µg/mL purified anti-eIF5A antibody (clone W19057C) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 649203) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular weight marker.
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945601 25 µg 95€
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945602 100 µg 235€
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Description

Eukaryotic initiation factor 5A (eIF5A) is a translation factor that aids in the process of translation elongation. Binding to the “E” site of the ribosome, eIF5A helps promote the synthesis of nascent polyproline motifs whose characteristic side-chain rigidity would otherwise prevent efficient translation and cause ribosomal stalling. Efficient polyproline translation requires post-translational hypusination of eIF5A at lysine residue 50, a modification unique to eIF5A and eIF5A2. eIF5A is required for normal autophagic flux due to the presence of a polyproline motif in the TFEB transcription factor. Low levels of the hypusine precursor spermidine are linked to increased aging, causing decreased TFEB synthesis and autophagic processes by way of downregulated eIF5A hypusination. eIF5A has been implicated in pancreatic ductal adenocarcinoma (PDAC), the most common form of pancreatic cancer. Here, eIF5A is hypothesized to increase the expression of migration-associated proteins, ultimately resulting in PDAC metastasis.  eIF5A has also been connected to HIV-1 pathogenesis by promoting its replication and is a potential target for antiretroviral therapy.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Synthetic peptide corresponding to human eIF5A
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.1 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone was tested for ICC using 4% PFA-fixed HeLa cells and NIH/3T3 cells permeabilized with either methanol or Triton X-100, and methanol-fixed and permeabilized HeLa cells and NIH/3T3 cells. None of these methods were compatible with eIF5A staining.

This clone failed to enrich eIF5A from HeLa extracts when tested for IP.

This clone may cross-react with eIF5A2 due to high homology between the immunizing sequence and the corresponding region of eIF5A2.

RRID
AB_2892514 (BioLegend Cat. No. 945601)
AB_2892514 (BioLegend Cat. No. 945602)

Antigen Details

Structure
EIF5A is a 154 amino acid protein with a predicted molecular weight of 17 kD.
Distribution

Ubiquitously expressed/Cytosol

Function
Translational elongation
Biology Area
Cell Biology, Protein Synthesis
Antigen References
  1. Fujimura K, et al. 2015. J Biol Chem. 295:29907-19.
  2. Gutierrez E, et al. 2013. Mol Cell. 51:35-45.
  3. Hoque M, et al. 2009. Retrovirology. 6:90.
  4. Zhang H, et al. 2019. Mol Cell. 76:110-125.
Gene ID
1984 View all products for this Gene ID
UniProt
View information about eIF5A on UniProt.org
Go To Top Version: 1    Revision Date: 03.31.2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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