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Purified anti-human/mouse Galectin-9 Antibody

Pricing & Availability
Clone
W21101F (See other available formats)
Regulatory Status
RUO
Other Names
Lgals9, Gal-9, Ecalectin, Tumor antigen HOM-HD-21, Ecalectin
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A_W21101F_PURE_Galectin9_1_060724
Human peripheral blood lymphocytes were stimulated with Cell Activation Cocktail (without Brefeldin A) (Cat. No. 423301) for 4 hours and surface stained with anti-human CD3 (clone UCHT1) APC. Cells were then fixed and permeabilized using the Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803), and intracellularly stained with purified anti-human/mouse Galectin-9 (clone W21101F) (left) or purified mouse IgG1, κ isotype control (right), followed by goat anti-mouse IgG PE.
  • A_W21101F_PURE_Galectin9_1_060724
    Human peripheral blood lymphocytes were stimulated with Cell Activation Cocktail (without Brefeldin A) (Cat. No. 423301) for 4 hours and surface stained with anti-human CD3 (clone UCHT1) APC. Cells were then fixed and permeabilized using the Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803), and intracellularly stained with purified anti-human/mouse Galectin-9 (clone W21101F) (left) or purified mouse IgG1, κ isotype control (right), followed by goat anti-mouse IgG PE.
  • B_W21101F_PURE_Galectin9_2_060724
    MOLT-4 (human T-lymphoblastic leukemia cell line) cells were fixed and permeabilized using the Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803), then intracellularly stained with purified anti-human/mouse Galectin-9 (clone W21101F) (filled histogram) or purified mouse IgG1, κ isotype control (open histogram), followed by goat anti-mouse IgG PE.
  • C_W21101F_PURE_Galectin9_3_070324
    C57BL/6 mouse thymocytes were fixed and permeabilized using the Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803), then intracellularly stained with purified anti-human/mouse Galectin-9 (clone W21101F) (left) or purified mouse IgG1, κ isotype control (right), followed by goat anti-mouse IgG PE. Cells were then co-stained with anti-mouse CD4 (clone GK1.5) APC.
  • D_W21101F_PURE_Galectin9_4_070324
    THP-1 cells, a human monocyte cell line isolated from peripheral blood of an acute monocytic leukemia patient, were fixed with 4% PFA Fixation Buffer (Cat. No. 420801) and permeabilized with 0.5% Triton X-100 for 10 minutes, and blocked with 5% FBS for 1 hour at room temperature. The cells were then stained without (panel A) and with (panel B) 5 μg/mL of the purified anti-human/mouse Galectin-9 antibody overnight at 4°C followed by goat anti-mouse IgG (clone Poly4053) Alexa Fluor® 647 for an hour at room temperature. Nuclei were counterstained with DAPI. The image was captured with a 40X objective. Scale bar: 50 μm
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390804 100 µg 184€
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Description

Galectin-9 is a mammalian lectin with a molecular weight around 50 kD. It is a member of the β-galactoside-binding family. With two conserved carbohydrate recognition domains (CRDs), galectin-9 binds small β-galactosides as well as complex glycoconjugates. HAVCR2/TIM3 has been reported as one of its ligands. Galectin-9 may be retained intracellularly or transported to the cell surface where it can be cleaved to generate a soluble form. Galectin-9 is expressed by lymphocytes, dendritic cells, granulocytes, eosinophils, astrocytes, endothelial cells, fibroblasts, and thymus epithelial cells. It can be induced by cytokines in various cell types and is involved in cell aggregation, adhesion, chemotaxis, and apoptosis. Galectin-9 induces regulatory T cells and suppresses Th1 and Th17 responses.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human galectin-9 protein (a.a 2-323)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ICFC - Quality tested
FC, ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our Cyto-Fast™ Fix/Perm Buffer Set. For flow cytometric staining, the suggested use of this reagent is  ≤ 0.25 µg per million cells in 100 µL volume. For immunocytochemistry, a concentration of 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance in each application.

Application Notes
Clone W21101F does not block the staining of reference clone 9M1-3.
 
Recombinant protein completely blocks the staining of test clone W21101F.
RRID
AB_3662367 (BioLegend Cat. No. 390804)

Antigen Details

Structure
Member of the β-galactoside-binding lectin family. Molecular weight around 50 kD. Binds small β-galactosides as well as complex glycoconjugates with two conserved carbohydrate recognition domains (CRDs).
Distribution

Lymphocytes, dendritic cells, neutrophils, eosinophils, astrocytes, endothelial cells, fibroblasts, thymus stromal/epithelial cells

Function
Cell aggregation, adhesion, chemotaxis, apoptosis, suppression of Th1 and Th17 responses, and induction of regulatory T cells
Interaction
Astrocytes, dendritic cells, endothelial cells, eosinophils, epithelial cells, fibroblasts, lymphocytes, neutrophils, tregs
Ligand/Receptor
HAVCR2/TIM3
Cell Type
Astrocytes, Dendritic cells, Endothelial cells, Eosinophils, Epithelial cells, Fibroblasts, Lymphocytes, Neutrophils, Tregs
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology
Molecular Family
Adhesion Molecules, Immune Checkpoint Receptors
Antigen References
  1. Seki M, et al. 2008. Clin Immunol. 127:78-88.
  2. Tsuboi Y, et al. 2007. Clin Immunol. 124:221-33.
  3. Zhu C, et al. 2005. Nat Immunol. 6:1245-52.
  4. Dunphy JL, et al. 2002. J Biol Chem. 277:14916-24.
Gene ID
8795 View all products for this Gene ID
UniProt
View information about Galectin-9 on UniProt.org
Go To Top Version: 1    Revision Date: 06.07.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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