- Clone
- W20057G (See other available formats)
- Regulatory Status
- RUO
- Other Names
- IFN-alpha 2B, IFN-alphaA, IFNA2, IFNA2B
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
632252 | 100 µg | 336€ |
Interferons are divided into type I, II, and III. Type I IFNs (IFN-α and IFN-β) are most abundant in number, distribution, and expression. They are also highly conserved among mammals in both structure and function. IFN-α2 has been used in the treatment of cancer such as bladder cancer, hepatocellular carcinoma, and leukemia. IFN-α2 augments the suppressed immune functions in patients with head and neck squamous cell carcinoma (HNSCC). IFN-α2 initiated T and NK cell mediated cytotoxicity of tumor cells through IFNγ dependent and independent mechanisms. IFN-α2 enhances suppressed T cell cytotoxicity by stimulation of the perforin-granzyme B system (IFNγ dependent). IFN-α2 also induces the expression of perforin-granzyme B in NK cells (NK mediated cytotoxicity, IFNγ independent). In a preliminary study, IFN-α2 appears to be an effective immunostimulator and impacts the clinical outcome in tongue squamous cell carcinoma patients. IFN-α has been used in the treatment of chronic hepatitis C (CHC); nevertheless, IFN-α is relatively unstable and requires frequent parenteral administration. Pegylation of IFN-α, polyethylene glycol (PEG)-IFN-α, reduces in vitro activity but increase the stability and plasma half-life of IFN-α. Therefore, PEG-IFN-α has replaced IFN-α in CHC treatment.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Human Recombinant IFN-α2 Protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
Direct ELISA - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by Direct ELISA. The optimal concentration of this reagent for the specific application and the experiment design should be established by the end-user.
- Additional Product Notes
-
This product is intended for sandwich-based immunoassay development on various assay platforms requiring antibody pairs. This antibody (clone W20057G) could function as a capture antibody when paired with biotin anti-human IFN-α2 (Cat. No. 636253) (clone W21083E) or (Cat. No. 616753) (clone Poly5292) as the detection antibody and using Recombinant Human IFN-α2 protein (Cat. Nos. 592702, 592704, 592706, and 592708) as the assay standard.
- RRID
-
AB_3662350 (BioLegend Cat. No. 632252)
Antigen Details
- Distribution
-
IFN-α is ubiquitously expressed
- Function
- IFN-α stimulates cytotoxic T lymphocytes and natural killer cell function. IFN-α has immunomodulatory and antiviral properties. IFNα2a induces CXCL10 in human microvascular endothelial cells and pulmonary artery smooth muscle cells. It is induced by viral infection.
- Interaction
- Varieties of cells express the IFNα receptor (IFNAR).
- Ligand/Receptor
- IFNAR complex has two components, IFNAR1 and IFNAR2.
- Cell Type
- Embryonic Stem Cells
- Biology Area
- Cancer Biomarkers, Neuroinflammation, Neuroscience, Stem Cells
- Antigen References
-
- Bose A and Baral R. 2007. Immunol Lett. 108:68-77.
- Zhao W, et al. 2008. J Immunol. 180:5483-9.
- Badiger R, et al. 2012. Plos One. 7:e46779.
- Aghemo A, et al. 2010. Nat Rev Gastroenterol Hepatol. 7:485-94.
- Becker-Merok A, et al. 2013. Lupus. 22:155-63.
- Mukherjee KK, et al. 2012. Indian J Med Res. 136:54-9.
- Gene ID
- 3440 View all products for this Gene ID
- UniProt
- View information about IFN-alpha2 on UniProt.org
Related FAQs
Other Formats
View All IFN-α2 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-human IFN-α2 | W20057G | Direct ELISA |
Compare Data Across All Formats
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Purified anti-human IFN-α2
Direct ELISA showing purified anti-human IFN-α2 (clone W2005...
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