Purified anti-human IgE Antibody anti-IgE

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Clone: MHE-18 (See other available formats)
Regulatory Status: RUO
Other Names: Immunoglobulin E
Isotype: Mouse IgG1, κ
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MHE-18_Pure_IgE_Antibody_061918 — Overnight cultured human peripheral blood mononuclear cells stained with CD193/CCR3 (clone 5E8) APC and human IgE (clone MHE-18) Purified (left) or mouse IgG1, κ isotype control Purified (Right), followed by anti-mouse IgG1 PE (gated on lymphocyte population).

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325502

100 µg

90€

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Description

IgE is the seventh immunoglobulin made by B cells in the immune response.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human Ig cocktail
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
ELISA, IHC - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per 10⁶ cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: The MHE-18 antibody reacts with both soluble and membrane human immunoglobulin E (IgE). It does not react with other Ig isotypes. Some formats of the MHE-18 antibody can be used as primary or secondary reagent for immunofluorescent staining or ELISA analysis.
RRID: AB_830847 (BioLegend Cat. No. 325502)

Antigen Details

Distribution: B cells
Cell Type: B cells
Biology Area: Immunology
Gene ID: 3497 View all products for this Gene ID
UniProt: View information about IgE on UniProt.org

Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Other Formats

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Description	Clone	Applications
Purified anti-human IgE	MHE-18	FC,ELISA,IHC
Biotin anti-human IgE	MHE-18	FC,ELISA,IHC
PE anti-human IgE	MHE-18	FC
APC anti-human IgE	MHE-18	FC
PE/Cyanine7 anti-human IgE	MHE-18	FC
PerCP/Cyanine5.5 anti-human IgE	MHE-18	FC
Alexa Fluor® 647 anti-human IgE	MHE-18	FC
APC/Fire™ 750 anti-human IgE	MHE-18	FC
TotalSeq™-A0868 anti-human IgE	MHE-18	PG
APC/Cyanine7 anti-human IgE	MHE-18	FC
TotalSeq™-C0868 anti-human IgE	MHE-18	PG
TotalSeq™-B0868 anti-human IgE	MHE-18	PG

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Go To Top Version: 2 Revision Date: 01.29.2013

For Research Use Only. Not for diagnostic or therapeutic use.

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