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Purified anti-human MCP-1 Antibody

Pricing & Availability
Clone
5D3-F7 (See other available formats)
Regulatory Status
RUO
Other Names
Monocyte chemoattractant protein-1, Monocyte chemoattractant and activating factor (MCAF), JE, Small inducible cytokine A2 (SCYA2), HC-11, P6, Smooth muscle cell chemotactic factor (SMC-CF), CCL2
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A_5D3-F7_PURE_MCP-1_ELISA_062918
  • A_5D3-F7_PURE_MCP-1_ELISA_062918
  • B_5D3-F7_MCP-1_Purified_antibody_WB_012114.jpg
    Immunoprecipitation/Western blot analysis of capturing ability of MCP-1 antibody for 200ng/ml MCP-1 recombinant protein. Lane 1 was immunoprecipitated with Armenian hamster IgG isotype control antibody and lane 2 was immunoprecipitated with MCP-1 antibody (2H5) and lane 3 was immunoprecipitated with MCP-1(5D3-F7) . Western blot analysis was performed using mouse anti-MCP-1 antibody (5D3-F7).
  • C_5D3-F7_MCP-1_Purified_Antibody_WB_012214.jpg
    Western blot analysis of recombinant human MCP-1 (lane 1), and recombinant mouse MCP-1 (lane 2) using anti-MCP-1 antibody (5D7-F7).
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502601 50 µg 76€
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502602 500 µg 188€
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Description

Monocyte chemotactic protein-1 (MCP-1) also known as monocyte chemotactic and activating factor (MCAF) was identified based on its ability to chemoattract monocytes. Subsequently, MCP-1 has also been found to regulate adhesion molecule expression and cytokine production in monocytes. MCP-1 is identical to the product of the JE gene, a PDGF inducible gene. MCP-1 is a member of the beta (C-C) chemokine subfamily, known as CCL2. The 5D3-F7 antibody reacts with human monocyte chemoattractant protein-1 (MCP-1).

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human MCP-1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA Capture - Quality tested
WB - Verified
ELISA & ELISPOT Detection, ICFC, IHC, IP - Reported in the literature, not verified in house
Recommended Usage

Recommended Usage:  Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 2-8 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of MCP-1 recombinant protein ranging from 2000 to 31.3 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA or ELISPOT Detection1: The biotinylated 5D3-F7 antibody is useful as the detection antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the purified 2H5 antibody (Cat. No. 505902/505906) as the capture antibody.
ELISA or ELISPOT Capture: The purified 5D3-F7 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated 2H5 antibody (Cat. No. 505908) as the detection antibody. The LEAF™ purified antibody (Cat. No. 502607) is suggested for ELISPOT capture.
Additional reported applications (for the relevant formats) include: intracellular flow cytometry2, immunoprecipitation1,3, Western blotting1, and immunohistochemical staining1.

Application References

(PubMed link indicates BioLegend citation)
  1. Peri, G., et al. 1994. J. Immunol. Meth. 174:249. (IP, IHC, WB)
  2. Rezaie-Majd, A., et al. 2002. Arterioscler Thromb Vasc Biol. 22:1194. (ICFC)
  3. Hirsch, A., et al. 1999. J. Virol. 73:404. (IP)
Product Citations
  1. Huang HW, et al. 2021. Int J Mol Sci. 22:. PubMed
  2. Lima LG, et al. 2021. Nat Commun. 12:3543. PubMed
  3. Fang WB, et al. 2021. Sci Rep. 11:8708. PubMed
RRID
AB_315241 (BioLegend Cat. No. 502601)
AB_315241 (BioLegend Cat. No. 502602)

Antigen Details

Structure
CC chemokine; dimer; 13.8 kD (Mammalian)
Bioactivity
Chemoattractant; regulate adhesion molecule expression, cytokine production in monocytes; proliferation/activation of CC-chemokine activated killer cells
Cell Sources
Monocytes, vascular endothelial cells, smooth muscle, glomerular mesangial cells
Cell Targets
Monocytes, basophils, T cells, NK cells, immature dendritic cells
Receptors
CCR2; CCR5; CCR10; D6; possibly L-CCR
Cell Type
Embryonic Stem Cells
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience, Stem Cells
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Bischoff S, et al. 1992. J. Exp. Med. 175:1271.
3. Charo I, et al. 1994. P. Natl. Acad. Sci. USA 91:2752.
4. Jiang Y, et al. 1992. J. Immunol. 148:2423.

Regulation
Upregulated by IL-1, TNF-α, PDGF, TGF-β
Gene ID
6347 View all products for this Gene ID
UniProt
View information about CCL2 on UniProt.org

Other Formats

View All CCL2 Reagents Request Custom Conjugation
Description Clone Applications
PE anti-human MCP-1 5D3-F7 ICFC
Purified anti-human MCP-1 5D3-F7 ELISA Capture,WB,ELISA,ELISPOT Detection,ICFC,IHC,IP
Biotin anti-human MCP-1 5D3-F7 ELISA Detection,ELISPOT Detection,ICFC
APC anti-human MCP-1 5D3-F7 ICFC
PE/Cyanine7 anti-human MCP-1 5D3-F7 ICFC
Go To Top Version: 6    Revision Date: 08.23.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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