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Purified anti-MAP2 Antibody

Pricing & Availability
Clone
1D7-B9-C8-F6 (See other available formats)
Regulatory Status
RUO
Other Names
Microtubule-associated protein 2 (MAP2)
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A. 1D7-B9-C8-F6_PURE_MAP2_IHC-P_012924
IHC staining of Purified anti-MAP2 (clone 1D7-B9-C8-F6) on formalin-fixed paraffin-embedded human cerebellum tissue. Following antigen retrieval using Citrate Buffer, 10X (Cat. No. 420902), the tissue was incubated without (panel A) and with (panel B) 10 µg/mL of Purified anti-MAP2 (clone 1D7-B9-C8-F6) followed by incubation with Alexa Fluor® 647 goat anti-mouse IgG (Cat. No. 405322) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 40X objective. Scale bar: 50 µm
  • A. 1D7-B9-C8-F6_PURE_MAP2_IHC-P_012924
    IHC staining of Purified anti-MAP2 (clone 1D7-B9-C8-F6) on formalin-fixed paraffin-embedded human cerebellum tissue. Following antigen retrieval using Citrate Buffer, 10X (Cat. No. 420902), the tissue was incubated without (panel A) and with (panel B) 10 µg/mL of Purified anti-MAP2 (clone 1D7-B9-C8-F6) followed by incubation with Alexa Fluor® 647 goat anti-mouse IgG (Cat. No. 405322) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 40X objective. Scale bar: 50 µm
  • B. 1D7-B9-C8-F6_PURE_MAP2_WB_012924
    Whole cell extracts (15 µg total protein) from indicated cell lines and/or tissues were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1 µg/mL of Purified anti-MAP2 (clone 1D7-B9-C8-F6) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG (Cat. No. 405306). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control at a 1:50000 Dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
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631151 25 µg 140€
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631152 100 µg 352€
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Description

Microtubules are 25 nm diameter protein rods found in most kinds of eukaryote cells. They are polymerized from a dimeric subunit made of one α subunit and one β tubulin subunit. Microtubules are associated with a family of proteins called microtubule associated proteins (MAPs), which includes the protein τ (tau) and a group of proteins referred to as MAP1, MAP2, MAP3, MAP4 and MAP5. MAP2 undergoes alternative splicing which results in four isoforms: MAP2A, MAP2B, MAP2C and MAP2D. MAP2A and MAP2B result in proteins with an apparent molecular weight of ~280 kD. MAP2C and MAP2D are smaller molecular weight proteins which correspond protein bands running at ~70-75 kD on SDS-PAGE gels. All these MAP2 isoforms share a C-terminal core sequence which includes either three or four microtubule binding domains, which are very similar to those found in the related microtubule binding protein τ (tau). MAP2 isoforms are expressed only in neuronal cells and specifically in the perikarya and dendrites of these cells. Antibodies to MAP2 are therefore excellent markers for neuronal cells, their perikarya and neuronal dendrites.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse, Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant fragment of the N-terminus of human MAP2
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
WB - Verified
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg/mL is suggested. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. 

Application Notes

This antibody (clone 1D7-B9-C8-F6) works with both Sodium Citrate H.I.E.R. (Cat. No. 928502) and Tris-EDTA pH 9.0 (10mM Tris, 1mM EDTA) antigen retrieval buffers, however, signal to noise ratio is higher using Sodium Citrate.

This antibody (clone 1D7-B9-C8-F6) is not suitable for immunocytochemistry.

RRID
AB_3097544 (BioLegend Cat. No. 631151)
AB_3097544 (BioLegend Cat. No. 631152)

Antigen Details

Structure
MAP2 is a 1,827 amino acid protein with a predicted molecular weight of 199.5 kD.
Distribution

Cellular distribution: plasma membrane, cytoskeleton, nucleus, and cytosol
Tissue distribution: Predominantly in the nervous system

Function
Microtubule assembly
Interaction
MAP2 interacts with microtubules. It has also been reported to interact with KNDC1 via KIND2.
Cell Type
Neural Stem Cells
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
Microtubules
Antigen References
  1. De Camilli P, et al. 1984. Neuroscience. 11:817-46.
  2. Bloom GS, et al. 1984. J Cell Biol. 98:320-30.
  3. Maschietto M, et al. 2013. Front Cell Neurosci. 25:7. 
  4. La Via L,et al. 2013. Nucelic Acids Res. 41:617-31. 
  5. Ji B, et al. 2014. Hum Mol Genet. 23:5683-705.  
  6. Fu X, et al. 2013. J Neurosci. 33:709.
Gene ID
4133 View all products for this Gene ID
UniProt
View information about MAP2 on UniProt.org
Go To Top Version: 1    Revision Date: 01.29.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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