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Purified anti-mouse CD150 (SLAM) Antibody

Pricing & Availability
Clone
TC15-12F12.2 (See other available formats)
Regulatory Status
RUO
Other Names
Signaling Lymphocyte Activation Molecule (SLAM), IPO-3
Isotype
Rat IgG2a, λ
Ave. Rating
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Product Citations
publications
TC15-12F12dot2_Pure_957
C57BL/6 mouse splenocytes were stained with purified CD150 (clone TC15-12F12.2) (filled histogram) or rat IgG2a isotype control (open histogram), followed by anti-rat IgG FITC.
  • TC15-12F12dot2_Pure_957
    C57BL/6 mouse splenocytes were stained with purified CD150 (clone TC15-12F12.2) (filled histogram) or rat IgG2a isotype control (open histogram), followed by anti-rat IgG FITC.
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115901 50 µg 76€
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115902 500 µg 216€
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Description

CD150 is a 75-95 kD member of the immunoglobulin superfamily, also known as SLAM (signaling lymphocyte activation molecule) or IPO-3. CD150, a single chain type I transmembrane molecule, is expressed on thymocytes, T cell subsets, B cells, dendritic cells, and endothelial cells. The expression is upregulated upon activation. CD150 expression has been shown to be maintained on Th1 but not Th2 clones. T regulatory cells express a relatively high level of CD150. Antibodies against CD150 have been shown to augment IFN-γ production by Th1 cells, especially when co-stimulated through the TCR. CD150 associates with the src homology 2-domain-containing protein tyrosine phosphatase SHP-2, and this association is thought to be involved in signal transduction. In combination with CD48, CD150 is a useful marker for hematopoietic stem cell studies.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse SLAM-human IgG1 fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C
Application

FC - Quality tested
IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The TC15-12F12.2 antibody has been reported to enhance the production of IFN-? by Th1 cells stimulated through TCR. Additional reported applications (for the relevant formats) include: immunoprecipitaion17, enhancing IFN-? production by Th1 cells when stimulated with CD31, and inhibiting CD3 induced T cell proliferation6. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 115949 & 115950).

Application References

(PubMed link indicates BioLegend citation)
  1. Castro AG, et al. 1999. J. Immunol. 163:5860. (FC, Costim, IP)
  2. Forsberg EC, et al. 2005. PLoS Genet. 1:e28. (FC)
  3. Terrazas LI, et al. 2005. Int. J. Parasitol. 35:1349. (FC)
  4. Cannons JL, et al. 2006. J. Exp. Med. 203:1551. (FC)
  5. Umemoto T, et al. 2006. J. Immunol. 177:7733. (FC)
  6. Jordan MA, et al. 2007. J. Immunol. 178:1618. (FC, Block) PubMed
  7. Jung Y, et al. 2007. Blood 110:82. PubMed
  8. Pimanda JE, et al. 2007. Proc. Natl. Acad. Sci. USA 104:840.
  9. Sugiyama T, et al. 2007. Proc. Natl. Acad. Sci. USA 104:175.
  10. Kim I, et al. 2006. Blood 108:737. PubMed
  11. Ema H, et al. 2006. Nat Protoc. 1:2979. PubMed
  12. Fraser ST, et al. 2007. Blood 109:4616. PubMed
  13. Jung Y, et al. 2008. Stem Cells. 26:2042. Pubmed
  14. Song J, et al. 2010. Blood 115:2592. PubMed
  15. Cridland SO, et al. 2009. Blood Cell. Mol. Dis. 43:149. (FC) PubMed
  16. Morita Y, et al. 2010. J. Exp Med. 207:1173. PubMed
  17. Talaei N, et al. 2015. J. Immunol. 195(10):4623. PubMed
Product Citations
  1. Ho YH, et al. 2019. Cell Stem Cell. 25:407. PubMed
  2. Hu J, et al. 2019. Am J Transl Res. 3.043055556. PubMed
  3. Hutton C, et al. 2021. Cancer Cell. 39:1227. PubMed
  4. Patel R, et al. 2018. Stem Cells Transl Med. 0.647916667. PubMed
  5. Umemoto T, et al. 2006. J Immunol. 177:7733. PubMed
  6. Dienz O, et al. 2020. J Immunol. 204:1521. PubMed
  7. Jiang L, et al. 2018. J Exp Med. 215:1337. PubMed
  8. Dong Y, et al. 2020. Cell Prolif. 53:e12885. PubMed
  9. Shan Q, et al. 2017. Nat Immunol. 18:931. PubMed
  10. He LD, et al. 2021. EMBO Rep. 22:e52196. PubMed
  11. Zhong L, et al. 2022. JCI Insight. 7:. PubMed
  12. Anso E, et al. 2017. Nat Cell Biol. 1.218055556. PubMed
  13. Hsieh HY, et al. 2020. Cancer Sci. 111:2400. PubMed
  14. Verbiest T, et al. 2018. Leukemia. 32:1435. PubMed
  15. Gubin MM, et al. 2018. Cell. 175:1014. PubMed
  16. Speir M, et al. 2020. Nat Immunol. 21:54. PubMed
  17. McCausland M, et al. 2007. J Immunol. 178:817. PubMed
  18. Lin Z, et al. 2021. Stem Cells. 39:240. PubMed
  19. Xu W, et al. 2021. Immunity. 54(3):526-541.e7. PubMed
RRID
AB_313680 (BioLegend Cat. No. 115901)
AB_313680 (BioLegend Cat. No. 115902)

Antigen Details

Structure
Ig superfamily, 75-95 kD
Distribution

Thymocytes, T cell subset, B lymphocytes, dendritic cells, endothelial cells

Function
B cell and dendritic cell costimulation
Ligand/Receptor
CD150
Cell Type
B cells, Dendritic cells, Endothelial cells, T cells, Thymocytes, Tregs
Biology Area
Costimulatory Molecules, Immunology, Innate Immunity
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Cocks BG, et al. 1995. Nature 376:260.
2. Punnonen J, et al. 1997. J. Exp. Med. 185:993.
3. Sidorenko SP, et al. 1993. J. Immunol. 151:4614.

Gene ID
27218 View all products for this Gene ID
UniProt
View information about CD150 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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