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Purified anti-mouse CD16.2 (FcγRIV) Antibody

Pricing & Availability
Clone
9E9 (See other available formats)
Regulatory Status
RUO
Other Names
FcgRIV, Fcgr4, Fcrl3, CD16-2, FcgRIV, Fcgr3a, FcgammaRIV, 4833442P21Rik, Fc gamma RIV
Isotype
Armenian Hamster IgG
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Product Citations
publications
1_9E9_Purified_CD16.2_Antibody_FC_010815
C57BL/6 bone marrow cells were stained with purified CD16.2 (clone 9E9, filled histogram) or Armenian hamster IgG isotype control (open histogram), followed by anti-Armenian hamster IgG PE. Histograms are gated on the myeloid population.
  • 1_9E9_Purified_CD16.2_Antibody_FC_010815
    C57BL/6 bone marrow cells were stained with purified CD16.2 (clone 9E9, filled histogram) or Armenian hamster IgG isotype control (open histogram), followed by anti-Armenian hamster IgG PE. Histograms are gated on the myeloid population.
  • 2_9E9_PURE_CD16dot2_Antibody_IHC_1_102717
    C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/mL of CD16.2 (clone 9E9) purified, B220 (clone RA3-6B2) Alexa Fluor® 488 (blue) and CD3 (clone 17A2) Alexa Fluor® 647 (green) overnight at 4°C, followed by 2.5 µg/ml of anti-hamster IgG (clone Poly4055) Alexa Fluor® 594 (red) for two hours at room temperature.The image was captured with a 10X objective.
  • 3_9E9_PURE_CD16dot2_Antibody_IHC_2_102717
    C57BL/6 mouse frozen intestine section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/mL of CD16.2 (clone 9E9) purified and CD324 (clone DECMA-1) Alexa Fluor® 647 (green) overnight at 4°C, followed by 2.5 µg/ml of anti-hamster IgG (clone Poly4055) Alexa Fluor® 594 (red) for two hours at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 10X objective.
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149502 100 µg 193€
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Description

FcγRIV, also known as CD16.2, is an intermediate-affinity activating receptor for IgG2a and IgG2b. CD16.2 is the mouse homolog of human FcγRIIIA. CD16.2 is a low-affinity IgE receptor for all allotypes and the ligation of FcγRIV by antigen-IgE immune complexes promotes macrophage-mediated phagocytosis and is involved in lung inflammation.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
FCγR4 —EC domain fusion with IgG1 Fc
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. For immunohistochemical staining on frozen tissue sections, the suggested use of this reagent is 5.0 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats of this clone) include: blocking of Fc?RIV function1 and inhibition of immune complex binding1,2. The LEAF™ or Ultra-LEAF™ purified antibody (Endotoxin < EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (contact our custom solutions team).

Application References

(PubMed link indicates BioLegend citation)
  1. Mancardi DA, et al. 2008. J. Clin. Invest 118:3738. (FC, Block)
  2. Nimmerjahn F, et al. 2005. Immunity 23:41.
Product Citations
  1. Wong HS, et al. 2021. Cell. . PubMed
  2. Enam SF, et al. 2020. J Neuroinflammation. 0.845138889. PubMed
  3. Sundararaj K, et al. 2021. Immunology. 162:418. PubMed
  4. L'homme L, et al. 2020. Sci Rep. 10:21095. PubMed
  5. Waight JD, et al. 2018. Cancer Cell. 33:1033. PubMed
  6. Hofmann J, et al. 2021. Front Immunol. 11:599495. PubMed
  7. Baumann D, et al. 2020. Nat Commun. 1.969444444. PubMed
  8. Zhu YP et al. 2018. Cell reports. 24(9):2329-2341 . PubMed
RRID
AB_2565302 (BioLegend Cat. No. 149502)

Antigen Details

Structure
22.4 kD
Distribution

Expressed on peripheral blood leukocytes, spleen, colon, bone marrow, and the intestine

Function
Activating receptor for IgG2a and IgG2b via association with an ITAM bearing accessory.
Ligand/Receptor
IgG2a and IgG2b
Cell Type
B cells, Leukocytes
Biology Area
Immunology
Molecular Family
CD Molecules, Fc Receptors
Antigen References
  1. Mechetina LV, et al. 2002. Immunogenetics. 54:463-8.
  2. Nimmerjahn F, et al. 2005. Immunity. 23:41-51.
  3. Seeling M, et al. 2013. Proc. Natl. Acad. Sci. 110:10729-34.
Gene ID
246256 View all products for this Gene ID
UniProt
View information about CD16.2 on UniProt.org
Go To Top Version: 3    Revision Date: 08.22.2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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