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Purified anti-mouse CD31 (PECAM-1) Antibody

Pricing & Availability
Clone
W18222B (See other available formats)
Regulatory Status
RUO
Other Names
PECAM-1, EndoCAM
Isotype
Rat IgG2b, κ
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Product Citations
publications
1_W18222B_PURE_CD31_Antibody_062420
C57BL/6 mouse splenocytes were stained with purified CD31 (PECAM-1) (clone W18222B) (left) or purified rat IgG2b, κ isotype control (right) followed by anti-rat IgG2b PE and CD3 Alexa Fluor® 647.
  • 1_W18222B_PURE_CD31_Antibody_062420
    C57BL/6 mouse splenocytes were stained with purified CD31 (PECAM-1) (clone W18222B) (left) or purified rat IgG2b, κ isotype control (right) followed by anti-rat IgG2b PE and CD3 Alexa Fluor® 647.
  • 2_W18222B_PURE_CD31_PECAM-1_Antibody_IHC-F_1_073021.png
    C57BL/6 mouse frozen lymph node section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then, the section was stained with 10 µg/mL of purified CD31 (clone W18222B) overnight at 4°C, followed by 2.5 µg/mL of Alexa Fluor® 594 anti-rat IgG (clone Poly4054, red) for two hours at room temperature. The image was captured with a 10X objective.
  • 3_W18222B_PURE_CD31_PECAM-1_Antibody_IHC-F_2_073021.png
    C57BL/6 mouse frozen liver section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then, the section was stained with 10 µg/mL of purified CD31 (clone W18222B) overnight at 4°C, followed by 2.5 µg/mL of Alexa Fluor® 594 anti-rat IgG (clone Poly4054, red) for two hours at room temperature. The image was captured with a 10X objective.
  • 4_W18222B_PURE_CD31_PECAM-1_Antibody_IHC-F_3_073021.png
    C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then, the section was stained with 10 µg/mL of purified CD31 (clone W18222B) overnight at 4°C, followed by 2.5 µg/mL of Alexa Fluor® 594 anti-rat IgG (clone Poly4054, red) for two hours at room temperature. The image was captured with a 10X objective.
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160202 500 µg 329€
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Description

CD31 is a 130-140 kD glycoprotein, also known as platelet endothelial cell adhesion molecule (PECAM-1) and EndoCAM. It is a member of the Ig superfamily, expressed on endothelial cells, platelets, granulocytes, monocytes/macrophages, dendritic cells, and T and B cell subsets, and is critical for cell-cell interactions. The primary ligands for CD31 have been reported to be CD38 and the vitronectin receptor (αv β3 integrin, CD51/CD61). Other reported functions of CD31 are neutrophil emigration to sites of inflammation and angiogenesis.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse CD31 transfected cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. For immunohistochemistry on frozen tissue sections, a concentration range of 5.0 - 10.0 µg/mL is suggested.It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2876566 (BioLegend Cat. No. 160202)

Antigen Details

Structure
Ig superfamily, 130-140 kD
Distribution

Endothelial cells, platelets, granulocytes, monocytes/macrophages, dendritic cells, T and B cell subsets

Function
Adhesion
Ligand/Receptor
CD38, αV/β3 integrin
Cell Type
B cells, Dendritic cells, Endothelial cells, Granulocytes, Macrophages, Monocytes, Neutrophils, Platelets, T cells
Biology Area
Angiogenesis, Cell Adhesion, Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
  2. DeLisser HM, et al. 1994. Immunol Today. 15:490-5.
  3. Newman PJ, et al. 1990. Science. 247:1219-22.
Gene ID
18613 View all products for this Gene ID
UniProt
View information about CD31 on UniProt.org
Go To Top Version: 2    Revision Date: 07.30.2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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