- Clone
- 145-2C11 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- CD3ε, T3, CD3
- Isotype
- Armenian Hamster IgG
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
100345 | 100 µg | 67€ |
CD3ε is a 20 kD transmembrane protein, also known as CD3 or T3. It is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3ε forms a TCR complex by associating with the CD3δ, γ and ζ chains, as well as the TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Armenian Hamster
- Immunogen
- H-2Kb-specific mouse cytotoxic T lymphocyte clone BM10-37
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1.0 mg/ml
- Storage & Handling
- The CD3ε antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
CyTOF® - Verified - Recommended Usage
-
This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.
- Application Notes
-
Clone 145-2C11 is useful for in vitro blocking of target-specific CTL-mediated cell lysis1, as well as T cell activation assays, inducing proliferation and cytokine production1,2,7,12,16. It also induces apoptosis in immature thymocytes32, and in vivo T cell depletion8-10. Additional reported applications (for relevant formats of this clone) include: immunoprecipitation1, immunohistochemical staining14,15 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, Western blotting4, complement-mediated cytotoxicity6, in vitro and in vivo stimulation of T cells1,2,7,12,16, immunofluorescent staining5, and in vivo T cell depletion8-10. The 145-2C11 antibody has been reported to block the binding of 17A2 antibody to CD3 epsilon-specific T cells11. Clone 145-2C11 is not recommended for formalin-fixed paraffin embedded sections. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 100314). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 100340) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
- Additional Product Notes
-
Maxpar® is a registered trademark of Standard BioTools Inc
-
Application References
(PubMed link indicates BioLegend citation) -
- Leo O, et al. 1987. P. Natl. Acad. Sci. USA 84:1374. (IP, Activ, Block)
- Kruisbeek AM, et al. 1991. In Current Protocols in Immunology. 3.12.1. (Activ)
- Duke RC, et al. 1995. Current Protocols in Immunology. 3.17.1.
- Salvadori S, et al. 1994. J. Immunol. 153:5176. (WB)
- Payer E, et al. 1991. J. Immunol. 146:2536. (IF)
- Jacobs H, et al. 1994. Eur. J. Immunol. 24:934. (CMCD)
- Vossen ACTM, et al. 1995. Eur. J. Immunol. 25:1492. (Activ)
- Henrickson M, et al. 1995. Transplantation 60:828. (Deplete)
- Kinnaert P, et al. 1996. Transpl. Int. 9:386. (Deplete)
- Han WR, et al. 1999. Transpl. Immunol. 7:207. (Deplete)
- Miescher GC, et al. 1989. Immunol. Lett. 23:113. (Block)
- Terrazas LI, et al. 2005. Intl. J. Parasitology. 35:1349. (Activ)
- Ko SY, et al. 2005. J. Immunol. 175:3309.
- Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC-F)
- Tilley SL, et al. 2007. J. Immunol. 178:3208. (IHC-F)
- Wang W, et al. 2007. J. Immunol. 178:4885. (Activ)
- Xiao S, et al. 2007. J. Exp. Med. 204:1691.
- Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245. (FC) PubMed.
- Curtsinger JM, et al.2005. J. Immunol. 175:4392. PubMed
- Guo Y, et al. 2008. Blood 112:480. PubMed
- Kenna TJ, et al. 2008. Blood 111:2091.
- Perchonock CE, et al. 2007. J. Immunol. 179:1768. PubMed
- Perchonock GE, et al. 2006. Mol. Cell. Biol. 26:6005. PubMed
- Kanaya T, et al. 2008. Am. J. Physiol. Gastrointest. Liver Physiol. 295:G273. PubMed
- de Koning BA, et al. 2006. Int. Immunol. 18:941. PubMed
- Schulteis RD, et al. 2008. Blood 295:G273. PubMed
- Qi Q, et al. 2009. Blood 114:564. PubMed
- Helmersson S, et al. 2013. Am J Pathol. 9440:123. Pubmed
- Wu S, et al. 2014. Clin Vaccine Immunol. 21:156. PubMed
- Yan J, et al. 2014. Vaccine. 32:2833. PubMed
- Guiterrez DA, et al. 2014. Diaebetes. 63:3827. PubMed
- Shi YF, et al. 1991. J Immunol. 146:3340. (Apop)
- Product Citations
-
- RRID
-
AB_2563748 (BioLegend Cat. No. 100345)
Antigen Details
- Structure
- Ig superfamily, forms CD3/TCR complex with CD3δ, γ and ζ subunits and TCR (α/β and γ/δ), 20 kD
- Distribution
-
Thymocytes (differentiation dependent), mature T cells, NK-T cells
- Function
- TCR signal transduction, T cell activation, antigen recognition
- Ligand/Receptor
- Peptide antigen/MHC-complex
- Cell Type
- NKT cells, T cells, Thymocytes, Tregs
- Biology Area
- Immunology
- Molecular Family
- CD Molecules, TCRs
- Antigen References
-
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Davis MM. 1990. Annu. Rev. Biochem. 59:475.
3. Weiss A, et al. 1994. Cell 76:263. - Gene ID
- 12501 View all products for this Gene ID
- UniProt
- View information about CD3epsilon on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?
-
We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm®. Please contact Fluidigm® directly for additional data and further details.
- Can I use Maxpar® Ready format clones for flow cytometry staining?
-
We have not tested the Maxpar® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.
- I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.
-
We only supply the antibody and not test that in house. Please contact Fluidigm® directly for troubleshooting advice: http://techsupport.fluidigm.com/
- Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?
-
The Maxpar® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.
Other Formats
View All CD3ε Reagents Request Custom ConjugationCustomers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
APC anti-mouse CD3ε
C57BL/6 mouse splenocytes were stained with CD3e (clone 145-... -
Biotin anti-mouse CD3ε
C57BL/6 mouse splenocytes were stained with biotinylated CD3... C57BL/6 mouse frozen spleen section was fixed with 4% parafo... -
FITC anti-mouse CD3ε
C57BL/6 mouse splenocytes were stained with CD3e (clone 145-... -
PE anti-mouse CD3ε
C57BL/6 mouse splenocytes were stained with CD3e (clone 145-... C57BL/6 mouse splenocytes were stained with CD3e (clone 145-... -
PE/Cyanine5 anti-mouse CD3ε
C57BL/6 mouse splenocytes were stained with CD3e (clone 145-... -
Purified anti-mouse CD3ε
C57BL/6 mouse splenocytes were stained with purified CD3e (c... C57BL/6 mouse frozen spleen section was fixed with 4% parafo... Fresh, frozen mouse spleen was stained with purified CD3ε cl... -
PE/Cyanine7 anti-mouse CD3ε
C57BL/6 mouse splenocytes were stained with CD3e (clone 145-... -
Alexa Fluor® 488 anti-mouse CD3ε
C57BL/6 mouse splenocytes were stained with CD3e (clone 145-... -
Alexa Fluor® 647 anti-mouse CD3ε
C57BL/6 mouse splenocytes were stained with CD3e (clone 145-... C57BL/6 mouse frozen spleen section was fixed with 4% parafo... Paraformaldehyde-fixed (4%), 500 μm-thick mouse spleen secti... -
PerCP anti-mouse CD3ε
C57BL/6 mouse splenocytes were stained with CD3e (clone 145-... -
PerCP/Cyanine5.5 anti-mouse CD3ε
C57BL/6 splenocytes were stained with CD45R/B220 APC and CD3... -
Purified anti-mouse CD3ε (Maxpar® Ready)
Mouse splenocytes stained with 152Sm anti-CD3e (145-2C11) an... -
APC/Cyanine7 anti-mouse CD3ε
C57BL/6 mouse splenocytes were stained with CD3e (clone 145-... -
Pacific Blue™ anti-mouse CD3ε
C57BL/6 mouse splenocytes were stained with CD3e (clone 145-... -
Brilliant Violet 421™ anti-mouse CD3ε
C57BL/6 mouse splenocytes were stained with CD19 FITC and CD... C57BL/6 mouse splenocytes were fixed with 2% paraformaldehyd... -
Ultra-LEAF™ Purified anti-mouse CD3ε
C57BL/6 mouse splenocytes were stained with LEAF™ purified C... -
PE/Dazzle™ 594 anti-mouse CD3ε
C57BL/6 mouse splenocytes were stained with CD3ε (cl... -
Brilliant Violet 510™ anti-mouse CD3ε
C57BL/6 mouse splenocytes were stained with CD3ε (cl... -
Brilliant Violet 605™ anti-mouse CD3ε
C57BL/6 mouse splenocytes were stained with CD3ε (cl... -
Brilliant Violet 711™ anti-mouse CD3ε
C57BL/6 mouse splenocytes were stained with CD3ε (cl... -
Brilliant Violet 785™ anti-mouse CD3ε
C57BL/6 mouse splenocytes were stained with CD3ε (cl... -
APC/Fire™ 750 anti-mouse CD3ε
C57BL/6 splenocytes were stained with CD19 FITC and CD3&epsi... -
GoInVivo™ Purified anti-mouse CD3ε
-
Spark YG™ 593 anti-mouse CD3
C57BL/6 splenocytes were stained with anti-mouse CD45R/B220 ... -
PerCP/Fire™ 780 anti-mouse CD3ε
C57BL/6 mouse splenocytes were stained with anti-mouse CD19... -
Brilliant Violet 650™ anti-mouse CD3ε
C57BL/6 mouse splenocytes were stained with anti-mouse/human...
Follow Us