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Purified anti-mouse CD4 Antibody

Pricing & Availability
Clone
H129.19 (See other available formats)
Regulatory Status
RUO
Other Names
L3T4, T4
Isotype
Rat IgG2a, κ
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Product Citations
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130302 500 µg 161€
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Description CD4 is a 55 kD protein, also known as L3T4 or T4. It is a member of the Ig superfamily, primarily expressed on most thymocytes, a subset of T cells, and weakly on macrophages and dendritic cells. It acts as a coreceptor with the TCR during T cell activation and thymic differentiation by binding MHC class II and associating with the protein tyrosin kinase, lck. The H129.19 antibody is able to block CD4 mediated cell adhesion and T cell activation. It is also useful for in vivo and in vitro depletion of CD4+ T cells. H129.19 antibody blocks binding of the anti-mouse CD4 mAbs GK1.5 and RM4-5, but not RM4-4 antibody. H129.19 antibody inhibits various responses of T helper cells to antigenic or mitogenic stimuli. Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse lymphocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC - Reported in the literature, not verified in house
Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes

Additional reported applications (for the relevant formats) include: blocking of CD4+ T cell activation1-3, depletion. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays.

Application References

(PubMed link indicates BioLegend citation)
  1. Naquet P. 1985. Immunogenetics. 22:247.
  2. Pierres A. 1984. J. Immunol.. 132:2775.
  3. Guo MW. 1995. Zygote. 3:65.
  4. Ghobrial RR. 1989. Clin Immunol Immunopathol. 52:486.
Product Citations
  1. Song J, et al. 2023. iScience. 26:106753. PubMed
  2. Wolf Y, et al. 2017. J Exp Med. 214:905. PubMed
  3. Park HJ, et al. 2019. Pharmaceutics. 0.780555556. PubMed
  4. Lin J, et al. 2017. Nat Commun. . 10.1038/s41467-017-01477-5. PubMed
RRID
AB_1279242 (BioLegend Cat. No. 130302)

Antigen Details

Structure
Ig superfamily, 55 kD
Distribution
Majority of thymocytes, T cell subset
Function
TCR co-receptor, T cell activation
Ligand/Receptor
MHC class II molecule
Cell Type
Thymocytes, T cells, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Bierer BE, et al. 1989. Annu. Rev. Immunol. 7:579.
3. Janeway CA. 1992. Annu. Rev. Immunol. 10:645.
4. Naquet P. 1985. Immunogenetics. 22:247-256.
5. Pierres A. 1984. J. Immunol.. 132:2775-2782.
6. Guo MW. 1995. Zygote. 3:65-73.
7. Ghobrial RR. 1989. Clin Immunol Immunopathol. 52:486-506.
Gene ID
12504 View all products for this Gene ID
UniProt
View information about CD4 on UniProt.org

Related FAQs

I am unable to see expression of T cell markers such as CD3 and CD4 post activation.
TCR-CD3 complexes on the T-lymphocyte surface are rapidly downregulated upon activation with peptide-MHC complex, superantigen or cross-linking with anti-TCR or anti-CD3 antibodies. PMA/Ionomycin treatment has been shown to downregulate surface CD4 expression. Receptor downregulation is a common biological phenomenon and so make sure that your stimulation treatment is not causing it in your sample type.
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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