Purified anti-mouse CD45 Maxpar Ready Antibody anti-CD45

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Clone: 30-F11 (See other available formats)
Regulatory Status: RUO
Other Names: T200, Ly-5, LCA
Isotype: Rat IgG2b, κ
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Product Citations: publications

30F11_Purified_CD45_CyTOF_112113 — Mouse splenocytes stained with 175Lu-anti-CD45 (30-F11) and 169Tm-anti-TCRb (H57-597). Data provided by DVS Sciences.

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103141

100 µg

76€

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Description

CD45 is a 180-240 kD glycoprotein also known as the leukocyte common antigen (LCA), T200, or Ly-5. It is a member of the protein tyrosine phosphatase (PTP) family, expressed on all hematopoietic cells except mature erythrocytes and platelets. There are different isoforms of CD45 that arise from variable splicing of exons 4, 5, and 6, which encode A, B, and C determinants, respectively. CD45 plays a key role in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation state of the cell as well as cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse thymus or spleen
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 1.0 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
CyTOF® - Verified
Recommended Usage: This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.
Application Notes: Clone 30-F11 reacts with all isoforms and both CD45.1 and CD45.2 alloantigens of CD45.

Additional reported applications (for relevant formats) include: immunoprecipitation³, complement-dependent cytotoxicity^1,5, immunohistochemistry (acetone-fixed frozen sections, zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections)^4,6, Western blotting⁷, and spatial biology (IBEX)^10,11. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 103163 and 103164).
Additional Product Notes: Maxpar® is a registered trademark of Standard BioTools Inc.
RRID: AB_2562800 (BioLegend Cat. No. 103141)

Antigen Details

Structure: Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution: All hematopoietic cells except mature erythrocytes and platelets
Function: Phosphatase, T and B cell activation
Ligand/Receptor: Galectin-1, CD2, CD3, CD4, TCR, CD22, Thy-1
Cell Type: B cells, Dendritic cells, Mesenchymal Stem Cells, Tregs
Biology Area: Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family: CD Molecules
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.
Gene ID: 19264 View all products for this Gene ID
UniProt: View information about CD45 on UniProt.org

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Cell Surface Flow Cytometry Staining Protocol

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Related FAQs

Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?

We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm^®. Please contact Fluidigm^® directly for additional data and further details.

http://techsupport.fluidigm.com/

Can I use Maxpar® Ready format clones for flow cytometry staining?

We have not tested the Maxpar^® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.

I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.

We only supply the antibody and not test that in house. Please contact Fluidigm^® directly for troubleshooting advice: http://techsupport.fluidigm.com/

Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?

The Maxpar^® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.

Other Formats

View All CD45 Reagents Request Custom Conjugation

Description	Clone	Applications
APC anti-mouse CD45	30-F11	FC
Biotin anti-mouse CD45	30-F11	FC
FITC anti-mouse CD45	30-F11	FC
PE anti-mouse CD45	30-F11	FC
PE/Cyanine5 anti-mouse CD45	30-F11	FC
Purified anti-mouse CD45	30-F11	FC,IHC-F,CyTOF®,IP,CMCD,IHC,WB
PE/Cyanine7 anti-mouse CD45	30-F11	FC
APC/Cyanine7 anti-mouse CD45	30-F11	FC
Alexa Fluor® 488 anti-mouse CD45	30-F11	FC,SB
Alexa Fluor® 647 anti-mouse CD45	30-F11	FC,ICC,IHC,3D IHC,SB
Pacific Blue™ anti-mouse CD45	30-F11	FC
Alexa Fluor® 700 anti-mouse CD45	30-F11	FC,SB
PerCP/Cyanine5.5 anti-mouse CD45	30-F11	FC
PerCP anti-mouse CD45	30-F11	FC
Alexa Fluor® 594 anti-mouse CD45	30-F11	IHC-F,FC,3D IHC
Brilliant Violet 421™ anti-mouse CD45	30-F11	FC,SB
Brilliant Violet 570™ anti-mouse CD45	30-F11	FC
Brilliant Violet 510™ anti-mouse CD45	30-F11	FC
Brilliant Violet 605™ anti-mouse CD45	30-F11	FC
Purified anti-mouse CD45 (Maxpar® Ready)	30-F11	FC,CyTOF®
PE/Dazzle™ 594 anti-mouse CD45	30-F11	FC
Brilliant Violet 711™ anti-mouse CD45	30-F11	FC
Brilliant Violet 785™ anti-mouse CD45	30-F11	FC
Brilliant Violet 650™ anti-mouse CD45	30-F11	FC
APC/Fire™ 750 anti-mouse CD45	30-F11	FC
Brilliant Violet 750™ anti-mouse CD45	30-F11	FC
TotalSeq™-A0096 anti-mouse CD45	30-F11	PG
TotalSeq™-B0096 anti-mouse CD45	30-F11	PG
Ultra-LEAF™ Purified anti-mouse CD45	30-F11	FC,CyTOF®,IP,CMCD,IHC,WB
Spark Blue™ 550 anti-mouse CD45	30-F11	FC
Spark NIR™ 685 anti-mouse CD45	30-F11	FC
TotalSeq™-C0096 anti-mouse CD45	30-F11	PG
Spark YG™ 570 anti-mouse CD45	30-F11	IHC-F
PE/Fire™ 640 anti-mouse CD45	30-F11	FC
APC/Fire™ 810 anti-mouse CD45	30-F11	FC
PE/Fire™ 700 anti-mouse CD45	30-F11	FC
Spark Violet™ 538 anti-mouse CD45	30-F11	FC
Spark YG™ 593 anti-mouse CD45	30-F11	FC
Spark Blue™ 574 anti-mouse CD45 Antibody	30-F11	FC
Spark Blue™ 515 anti-mouse CD45	30-F11	FC
Spark UV™ 387 anti-mouse CD45	30-F11	FC
PE/Fire™ 810 anti-mouse CD45	30-F11	FC
Spark Red™ 718 anti-mouse CD45 (Flexi-Fluor™)	30-F11	FC
Spark PLUS UV395™ anti-mouse CD45	30-F11	FC

Certificate of Analysis

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Go To Top Version: 3 Revision Date: 07.21.2022

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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