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Purified anti-mouse GITR Ligand Antibody

Pricing & Availability
Clone
YGL 386 (See other available formats)
Regulatory Status
RUO
Other Names
AITRL, TNFSF18, TL6, GITRL
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
Cat # Size Price Quantity Check Availability Save
120302 200 µg 235€
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Description

GITR ligand (GITRL) is a member of the TNF superfamily, also known as TNFSF18 or TL6. It is expressed on B cells, macrophages, dendritic cells and endothelial cells. The expression of GITRL on dendritic cell surface can be transiently up-regulated and then rapidly down-regulated by LPS stimulation. GITRL binds to its receptor, GITR, to co-stimulate T cell activation, proliferation, and cytokine production, and inhibit the suppressive functions of CD4+CD25+ Treg cells, as well as induce macrophages activation. GITR-GITRL interaction has been implicated as an essential regulator of immune response to self antigens and phathogens.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant mouse GITRL protein and Transfected cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Tone M, et al. 2003. Proc. Natl. Acad. Sci. USA 100:15059
  2. Lu LF, et al. 2005. J. Immunol. 175:1651
  3. Esparza EM and RH Arch. 2005. J. Immunol. 174:7875
  4. Ray A, et al. 2012. J. Immunol. 188:3188. PubMed.
Product Citations
  1. Li H, et al. 2022. Cancer Cell. 40:36. PubMed
RRID
AB_2207249 (BioLegend Cat. No. 120302)

Antigen Details

Structure
TNF superfamily member
Distribution

B cells, macrophages, dendritic cells, endothelial cells.

Function
Bind GITR receptor to co-stimulate T cells activation, proliferation, and cytokine production; inhibit the suppressive activities of CD4+CD25+ Treg cells.
Ligand/Receptor
GITR (TNFRSF18)
Cell Type
B cells, Dendritic cells, Endothelial cells, Macrophages, Tregs
Biology Area
Immunology
Antigen References

1. Tone M, et al. 2003. Proc. Natl. Acad. Sci. USA 100:15059.
2. Kim JD, et al. 2003. Genes Immun. 4:564.
3. Yu KY, et al. 2003. Biochem. Biophys. Res. Commun. 310:433.
4. Shin HH, et al. 2002. Cytokine 19:187.

Gene ID
240873 View all products for this Gene ID
UniProt
View information about GITRL on UniProt.org
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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