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Purified anti-mouse Ly-6A/E (Sca-1) Antibody

Pricing & Availability
Clone
D7 (See other available formats)
Regulatory Status
RUO
Other Names
Sca-1
Isotype
Rat IgG2a, κ
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Product Citations
publications
D7_Purified_011108
C57BL/6 splenocytes stained with purified D7, followed by anti-rat IgG FITC
  • D7_Purified_011108
    C57BL/6 splenocytes stained with purified D7, followed by anti-rat IgG FITC
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108101 50 µg 48€
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108102 500 µg 184€
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Description

Ly-6A/E, also known as Sca-1, is an 18 kD member of the Ly-6 multigene family. Ly6A/E is a glycosylphosphatidylinositol (GPI)-linked protein expressed on hematopoietic stem cells. In mice expressing the Ly-6.2 haplotype (e.g., AKR, C57BL, C57BR, DBA/2, SJL, SWR, and 129), Ly-6A/E is also expressed on peripheral B lymphocytes and thymic and peripheral T lymphocytes. Strains expressing the Ly-6.1 haplotype (e.g., BALB/c, CBA, C3H/He, DBA/1, and NZB) have low Ly-6A/E expression on resting peripheral lymphocytes. The expression of Ly-6A/E on lymphocytes is upregulated upon activation from both Ly6.1 and Ly6.2 haplotype mice. Ly-6A/E is thought to be involved in the regulation of both T and B cell responses.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
IL-2-dependent mouse T-cell line (CTL-L)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
WB, IP, IHC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The D7 antibody has been reported to induce T cell activation and inhibit TCR-induced IL-2 production. Additional reported applications (for the relevant formats) include: Western blotting1,2, immunoprecipitation1, in vitro lymphocyte activation3-6, induction of redirected lysis7, induction of T cell inhibitory signalling8, immunofluorescence9, and immunohistochemical staining of acetone-fixed frozen sections13 and Bouin-fixed, paraffin-embedded samples9.

The two Sca-1 recognizing clones D7 and E13-161.7 have been shown to bind distinct epitopes due to the inability of D7 to block the binding of E13-161.7.14 

Application References

(PubMed link indicates BioLegend citation)
  1. Ortega G, et al. 1986. J. Immunol. 137:3240. (WB, IP)
  2. Palfree RGE, et al. 1986. Immunogenetics 23:197. (WB)
  3. Codias EK, et al. 1990. J. Immunol. 144:2197.
  4. Malek TR, et al. 1986. J. Exp. Med. 164:709.
  5. Codias EK, et al. 1990. J. Immunol. 145:1407.
  6. Ivanov V, et al. 1994. J. Immunol. 153:2394.
  7. Karlhofer FM, et al. 1991. J. Immunol. 146:3662.
  8. Fleming T, et al. 1994. J. Immunol. 153:1955.
  9. van Bragt MPA, et al. 2005. Biol. Reprod. 73:634. (IF, IHC)
  10. Umland O, et al. 2007. J. Immunol. 178:4147.
  11. Cridland SO, et al. 2009. Blood Cell. Mol. Dis. 45:149. (FC) PubMed
  12. Pronk CJ, et al. 2011. J. Exp Med. PubMed
  13. English A, et al. 2000. J. Immunol. 165:3763. (IHC)
  14. Bamezai A and Rock KL. 1995. Proc. Natl. Acad. Sci. USA 92:4294.
  15. Wiesner DL, et al. 2015. PLoS Pathog. 11:1004701. PubMed
Product Citations
  1. Blériot C, et al. 2020. Methods Mol Biol. 2164:87. PubMed
  2. Tremblay M, et al. 2020. Elife. 9: . PubMed
  3. Valet C, et al. 2022. J Clin Invest. 132: . PubMed
  4. Brassington K, et al. 2022. Front Immunol. 13:1040233. PubMed
  5. Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
  6. Weng L, et al. 2016. Sci Rep. 6:38632. PubMed
  7. Reeves PM, et al. 2017. Oncotarget. 8:94040. PubMed
  8. Paulo E, et al. 2018. Sci Rep. 8:11001. PubMed
  9. Hu X, et al. 2016. Nat Commun. 7:13095. PubMed
  10. Chen S, et al. 2022. Cancer Discov. :. PubMed
  11. Casey AE, et al. 2018. J Cell Biol. 217:2951. PubMed
  12. Inoue D, et al. 2021. Nat Genet. 53:707. PubMed
  13. Yoshimi A, et al. 2019. Nature. 574:273. PubMed
  14. Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
  15. Suzuki Y, et al. 2021. FEBS Open Bio. 11:2619. PubMed
  16. Cardoso F, et al. 2021. Nature. 597:410. PubMed
  17. Zhong X, et al. 2020. Proc Natl Acad Sci U S A. 8563:117. PubMed
  18. Park JW, et al. 2022. Cancer Med. 11:1925. PubMed
  19. Blriot C, et al. 2021. Immunity. :. PubMed
  20. Gubin MM, et al. 2018. Cell. 175:1014. PubMed
  21. Karmaus PWF, et al. 2019. Nature. 565:101. PubMed
  22. Pronk C, et al. 2011. J Exp Med. 208:1563. PubMed
  23. Krotenberg Garcia A, et al. 2021. Cell Reports. 36(1):109307. PubMed
  24. Oherle K, et al. 2020. Immunity. 275:52. PubMed
  25. Link CWM, et al. 2020. Front Immunol. 11:596772. PubMed
  26. Samala R, et al. 2013. J Proteomics. 75:5304. PubMed
  27. Iida Y, et al. 2020. J Immunother Cancer. 8:. PubMed
  28. Burton OT, et al. 2018. Clin Exp Allergy. 48:288. PubMed
  29. Silva J, et al. 2022. Nat Commun. 13:4492. PubMed
RRID
AB_313338 (BioLegend Cat. No. 108101)
AB_313338 (BioLegend Cat. No. 108102)

Antigen Details

Structure
Ly-6 multigene family, 18 kD
Distribution

Hematopoietic stem cells, activated T cells and B cells, subset of resting B cells and T cells

Function
Regulates B and T cell responses
Cell Type
B cells, Hematopoietic stem and progenitors, Mesenchymal Stem Cells, T cells
Biology Area
Immunology, Stem Cells
Antigen References

1. Rock KL, et al. 1989. Immunol. Rev. 111:195.
2. Morrison SJ, et al. 1994. Immunity 1:661.
3. Spangrude GJ, et al. 1988. J. Immunol. 141:3697.
4. Malek T, et al. 1986. J. Exp. Med. 164:709.

Gene ID
110454 View all products for this Gene ID
UniProt
View information about Ly-6A/E on UniProt.org
Go To Top Version: 3    Revision Date: 07.11.2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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