Western blot of purified anti-NrCAM antibody (clone N343/26). Lane 1: Molecular weight marker; Lane 2: 30 µg of human brain membrane lysate; Lane 3: 30 µg of mouse brain membrane lysate; Lane 4: 30 µg of rat brain membrane lysate; Lane 5: 30µg of SH-SY5Y cell lysate. The blot was incubated with 10 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
Western blot of purified anti-NrCAM antibody (clone N343/26). Lane 1: Molecular weight marker; Lane 2: 30 µg of human brain membrane lysate; Lane 3: 30 µg of mouse brain membrane lysate; Lane 4: 30 µg of rat brain membrane lysate; Lane 5: 30µg of SH-SY5Y cell lysate. The blot was incubated with 10 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
ICC staining of purified anti-NrCAM antibody (clone N343/26) on SH-SY5Y cells. The cells were fixed with methanol, permeabilized with a buffer containing 0.1% Triton X-100 and 0.25% BSA, and blocked with 2% normal goat serum and 0.02% BSA. The cells were then incubated with 10 µg/ml of the primary antibody overnight at 4°C, followed by incubation with 2.5 µg/ml of Alexa Fluor® 488 Goat anti-mouse IgG for one hour at room temperature. Nuclei were counterstained with DAPI. The image was captured with a 40X objective.
IHC staining of purified anti-NrCAM antibody (clone N343/26) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm.
IHC staining of purified anti-NrCAM antibody (clone N343/26) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
IHC staining of purified anti-NrCAM antibody (clone N343/26) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm.
NgCAM (Neuron-glia cell adhesion molecule)-related cell adhesion molecule (NrCAM), also known as Bravo, is an ankyrin-binding protein that modulates neuronal adhesion. NrCAM is part of the Ig superfamily and is involved in axonal cone growth.
This monoclonal antibody was raised against a fusion protein corresponding to amino acids 30-845 (extracellular domain) of mouse NrCAM.
Formulation
Phosphate-buffered solution (no preservatives or carrier proteins).
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 2.0-10 µg/ml. For immunocytochemistry, the suggested use of this reagent is 2.0-10 µg/ml. For immunohistochemistry, a concentration of 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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