IHC staining of purified anti-Pan-Cytokeratin antibody (clone AE-1/AE-3) on formalin-fixed paraffin-embedded human skin tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 1: Universal, 100X (Cat. No. 927901), the tissue was incubated with 1 µg/ml of the primary antibody for 20 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, AEC, Cat. No. 929401) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective.
IHC staining of purified anti-Pan-Cytokeratin antibody (clone AE-1/AE-3) on formalin-fixed paraffin-embedded human skin tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 1: Universal, 100X (Cat. No. 927901), the tissue was incubated with 1 µg/ml of the primary antibody for 20 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, AEC, Cat. No. 929401) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective.
Formalin-fixed paraffin-embedded human tonsil treated with a citrate buffer pH6 and heat for antigen retrieval was stained with purified Pan-Cytokeratin clone AE-1/AE-3, conjugated and detected with a Cy3 conjugated CODEX™ oligonucleotide duplex (red). Data generated at Akoya Biosciences, Inc. using the CODEX™ technology.
AE-1 immunoreacts with an antigenic determinant present on most of the subfamily A cytokeratins, including cytokeratins with molecular weights of 56.5, 50, 48 and 40 kD. AE-3 reacts with an antigenic determinant shared by the subfamily B cytokeratins including cytokeratins with molecular weights of of 64, 59, 58, 56, and 52 kD.
Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 0.5 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Product Citations
Metcalf KJ, et al. 2022. Matrix Biol Plus. 14:100105. PubMed
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