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Purified anti-Rat Blood-Brain Barrier Antibody

Pricing & Availability
Clone
SMI 71 (See other available formats)
Regulatory Status
RUO
Other Names
Endothelial barrier antigen, EBA
Isotype
Mouse IgM, κ
Ave. Rating
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Product Citations
publications
SMI-71_PURE_RBBB_Antibody_040318
IHC staining of purified anti-Rat Blood-Brain Barrier antibody (clone SMI 71) on formalin-fixed paraffin-embedded rat brain tissue. The tissue was incubated with the primary antibody at 10 µg/mL overnight at 4°C, followed by incubation with 2.5 µg/ml of Alexa Fluor® 594 conjugated anti-mouse IgG (Cat. No. 405326) for 1 hour at room temperature. Nuclei were counterstained with DAPI. The image was captured with a 40X objective. Scale bar: 50 µm
  • SMI-71_PURE_RBBB_Antibody_040318
    IHC staining of purified anti-Rat Blood-Brain Barrier antibody (clone SMI 71) on formalin-fixed paraffin-embedded rat brain tissue. The tissue was incubated with the primary antibody at 10 µg/mL overnight at 4°C, followed by incubation with 2.5 µg/ml of Alexa Fluor® 594 conjugated anti-mouse IgG (Cat. No. 405326) for 1 hour at room temperature. Nuclei were counterstained with DAPI. The image was captured with a 40X objective. Scale bar: 50 µm
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836803 25 µg 90€
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836804 100 µg 221€
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Description

SMI 71 is specific for a rat endothelial protein found in areas with blood-brain or blood-nerve barriers. The antibody does not react with endothelia of periventricular organs or with fenestrated endothelia in peripheral tissues. Specifically, it has been shown that staining with SMI 71 is not observed in blood vessels and sinusoids in the liver, vessels in the heart, adrenal, skeletal muscle, intestine, thymus, lymph nodes, pancreas, thyroid, or skin. However, a patchy reaction was observed on some vessel walls of the spleen and epidermis of the skin3. Reactivity with the antibody develops in newborn rats along with maturation of the blood brain barrier. Reactivity disappears in lesions of experimental allergic encephalomyelitis. Contrary to the belief that astrocytes and perhaps neurons are essential for the establishment of the blood-brain barrier, destruction of neurons (monitored by SMI 311 and anti-MAP2) and of astrocytes (monitored by anti-GFAP and anti-S100B) leads only to transient abolition of SMI 71 reactivity and only transient transendothelial passage of serum albumin.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Rat
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Ghabriel MN, et al. 2002. Reproduction 123:389-97. (IHC)
  2. Beaumont M, et. al. 2009. J. Cereb. Blood Flow Metab. 29:1714. (IHC) PubMed
  3. Sternberger NH, Sternberger LA. 1987. Proc. Natl. Acad. Sci. USA 84(22):8169. (IHC) PubMed
Product Citations
  1. Zhang L, et al. 2022. Front Aging Neurosci. 14:926485. PubMed
  2. Hubbard WB, et al. 2022. Acta Neuropathol Commun. 10:93. PubMed
RRID
AB_2687236 (BioLegend Cat. No. 836803)
AB_2687236 (BioLegend Cat. No. 836804)

Antigen Details

Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers
Gene ID
NA
UniProt
View information about Blood-Brain Barrier on UniProt.org
Go To Top Version: 3    Revision Date: 07.09.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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