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Purified anti-rat CD147 Antibody

Pricing & Availability
Clone
OX-47 (See other available formats)
Regulatory Status
RUO
Other Names
OX-47, CE-9
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
OX-47_Pure_021009.jpg
LOU rat splenocytes stained with purified OX-47, followed by anti-mIgG1 PE
  • OX-47_Pure_021009.jpg
    LOU rat splenocytes stained with purified OX-47, followed by anti-mIgG1 PE
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204601 100 µg 95€
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Description

CD147 is a 54 kD type I integral transmembrane protein, also called extracellular metalloproteinase inducer (EMMMPRIN) or M6 in humans, Basigin or gp42 in mouse, and OX-47 or CE-9 in rat. It is a heavily glycosylated Ig superfamily member and is expressed widely in hematopoietic and non-hematopoietic tissues. CD147 is an adhesion molecule and, through interaction with integrins, plays an important role in fetal, neuronal, blood cells and extracellular matrix development.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
T blasts from a mixed lymphocyte reaction
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F, WB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections1 and Western blotting2.

Application References

(PubMed link indicates BioLegend citation)
  1. Glenn M, et al. 1995. Circulation 92:1919. (IHC)
  2. Betsuyaku T, et al. 2003. Am. J. Respir. Cell Mol. Biol. 28:600. (WB)
Product Citations
  1. Qi Y, et al. 2020. Sci Rep. 18368:10. PubMed
RRID
AB_1186183 (BioLegend Cat. No. 204601)

Antigen Details

Structure
Single chain type I transmembrane 58 kd heavily glycosylated protein. N terminal extracellular part consists of two C2 type Ig-like domains which are highly glycosylated. Recently reported: isoform with an additional membrane-distal Ig-like domain.
Distribution

CD147 is expressed at varying levels in many cells types, including haematopoietic, epithelial, and endothelial cells. Expressed on immature thymocytes and at varying levels on spleen cells , bone marrow cells and macrophages.

Function
CD147 is implicated in multiple functions, such as matrix metalloproteinase induction, cell adhesion, retinal cell development, HIV attachment, embryonic development and T cell activation.
Ligand/Receptor
cyclophilin
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Glenn M, et al. 1995. Circulation 92:1919.
2. Betsuyaku T, et al. 2003. Am. J. Respir. Cell Mol. Biol. 28:600.

Gene ID
25246 View all products for this Gene ID
UniProt
View information about CD147 on UniProt.org
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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