Purified anti-rat CD44H (Pgp-1, H-CAM) Antibody

Pricing & Availability
Clone
OX-49 (See other available formats)
Regulatory Status
RUO
Other Names
Pgp-1, H-CAM, CD44s
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
OX-49_FITC_061909.jpg
LOU rat splenocytes stained with purified OX-49 conjugated to FITC
  • OX-49_FITC_061909.jpg
    LOU rat splenocytes stained with purified OX-49 conjugated to FITC
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203901 25 µg 52€
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Description

CD44 is an 80-95 kD cell surface glycoprotein. It is expressed on all leukocytes, endothelial cells, hepatocytes, and mesenchymal cells. It is up-regulated when T cells and B cells are activated. It was reported that CD44 is a valuable marker for memory T cells. CD44 is an adhesion molecule involved in leukocyte adhesion and homing to lymphoid organs. The OX-49 antibody reacts with CD44H (known as CD44s) expressed on most leukocytes, except for a subset of B lymphocytes. The epitope recognized by OX-49 antibody has been mapped to a region on both the standard, CD44s, and the splice variant, CD44v, isoforms of CD44. However it was reported that OX-49 antibody cannot detect the CD44V isoform, possibly due to conformational changes in the epitope.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Rat
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Rat T blasts from mixed lymphocyte reactions
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC, IP - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Paterson DJ, et al. 1987. Mol Immunol. 24(12):1281
Product Citations
  1. Yamada S, et al. 2021. J Tissue Eng. 12:20417314211019375. PubMed
RRID
AB_1659203 (BioLegend Cat. No. 203901)

Antigen Details

Structure
A 80-95 kD cell surface glycoprotein
Distribution

Expressed on most leukocytes. It is up-regulated when T cells and B cells are activated.

Function
Involve in leukocyte adhesion and homing to lymphoid organs.
Ligand/Receptor
Hyaluronate
Cell Type
B cells, Leukocytes, T cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Arch R, et al. 1992. Science 257 (5070):682
2. Mitnacht R, et al. 1995. Eur. J. Immunol. 25(2):328
3. Zheng Z, et al. 1995. J Cell Bio. 130(2):485
4. Westermann J, et al. 1994. Eur. J. Immunol. 24(10):2312

Gene ID
12505 View all products for this Gene ID
UniProt
View information about CD44H on UniProt.org
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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