- Clone
- W19081C (See other available formats)
- Regulatory Status
- RUO
- Other Names
- SAM and HD Domain Containing Deoxynucleoside Triphosphate, Triphosphohydrolase 1, Monocyte Protein 5, Aicardi-Goutieres Syndrome 5, Sterile Alpha Motif and Histidine Aspartate Domain-Containing Protein 1
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
941001 | 25 µg | 95€ | ||||
941002 | 100 µg | 235€ |
SAMHD1 is a host defense factor involved in the innate immune response to viral infections, including HIV-1. Through its dNTPase activity, SAMHD1 disrupts viral genome replication by depleting available pools of deoxynucleotide triphosphates (dNTPs) for viral reverse transcription in dendritic and myeloid cells. In addition to its role in innate immune defense, SAMHD1 also plays an essential role in preserving genome stability through maintaining balanced dNTP pools. Independently of its dNTPase activity, SAMHD1 is required for resection of stalled, gapped, or reversed DNA replication forks by stimulating the exonuclease activity of MRE11. SAMHD1-deficient cells show increased dNTPs and genome instability, and an increased accumulation of G1-positive cells. These cells also accumulate single-strand DNA fragments in the cytosol, leading to the induction of pro-inflammatory type 1 interferons through the cGAS-STING pathway. Mutations in the SAMHD1 are found in many cancers, as well as the severe congenital inflammatory disease Aicardi-Goutieres syndrome.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Partial recombinant human SAMHD1 protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
IP, ICFC - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.1 - 1.0 µg/mL. For immunoprecipitation, the suggested use of this reagent is 2.5 µg/test. For flow cytometric staining using our True-Phos™ Perm Buffer Protocol, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
During PD testing, we were not able to confirm mouse reactivity of W19081C by western blot.
When tested for western blot, W19081C detected a ~30 kD and 60 kD protein of unknown origin in THP-1 lysates.
Clone W19081C failed immunocytochemistry testing due to non-specific staining. We therefore do not recommend the clone for this application. - RRID
-
AB_2888902 (BioLegend Cat. No. 941001)
AB_2888902 (BioLegend Cat. No. 941002)
Antigen Details
- Structure
- SAMHD1 is a 626 amino acid protein with a predicted molecular weight of 72 kD.
- Distribution
-
Ubiquitously expressed/Nucleus
- Function
- Innate immune response, DNA replication and repair
- Cell Type
- Dendritic cells, Monocytes
- Biology Area
- Cell Biology, Cell Cycle/DNA Replication, DNA Repair/Replication, Innate Immunity
- Molecular Family
- Enzymes and Regulators
- Antigen References
-
- Laguette N, et al. 2011. Nature. 474:654.
- Gene ID
- 25939 View all products for this Gene ID
- UniProt
- View information about SAMHD1 on UniProt.org
Other Formats
View All SAMHD1 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-SAMHD1 | W19081C | WB,IP,ICFC |
PE anti-SAMHD1 | W19081C | ICFC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Purified anti-SAMHD1
Whole cell extracts (15 µg total protein) from the indicated... Whole cell extracts (250 µg total protein) prepared from Dau... Human peripheral blood mononuclear cells were fixed and perm... THP1 cells were fixed and permeabilized using True-Phos™ Per... -
PE anti-SAMHD1
Human peripheral blood mononuclear cells were surface staine... THP1 cells were fixed and permeabilized using True-Phos™ Per...
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