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Purified anti-SATB1 Antibody

Pricing & Availability
Clone
O96C6 (See other available formats)
Regulatory Status
RUO
Other Names
Special AT-rich sequence-binding protein 1, SATB homeobox 1, DNA-binding protein SATB1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
O96C6_Purified_SATB1_Antibody_1_WB_033115
Total cell lysate from Jurkat (lane 1, 15 µg), mouse thymus (lane 2, 15 µg) and HeLa (lane 3, 15 µg) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-SATB1 (clone O96C6) antibody. Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP (Cat. No. 405306) and chemiluminescence detection.
  • O96C6_Purified_SATB1_Antibody_1_WB_033115
    Total cell lysate from Jurkat (lane 1, 15 µg), mouse thymus (lane 2, 15 µg) and HeLa (lane 3, 15 µg) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-SATB1 (clone O96C6) antibody. Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP (Cat. No. 405306) and chemiluminescence detection.
  • O96C6_Purified_SATB1_Antibody_2_IF_033115
    Jurkat cells were stained with purified anti-SATB1 (clone O96C6) antibody, followed by staining with Alexa Fluor® 594 conjugated goat anti-mouse IgG antibody (red, Cat. No. 405326). Nuclei were counterstained with DAPI (blue). The image was captured with a 40X objective.
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676102 100 µg 231€
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Description

SATB1 is a global regulator that selectively tethers multiple genomic loci and recruits chromatin-remodeling enzymes (e.g. ACF, ISWI, HDAC1 and histone acetyltransferase) to control chromatin structure and gene expression. SATB1 also recruits corepressors or coactivators to promoters. These two opposing interactions, however, are determined by whether or not the SATB1 is phosphorylated by protein kinase C. Unphosphorylated and phosphorylated SATB1 preferably binds to PCAF and HDAC1, respectively. SATB1 also forms a regulatory complex with the promyelocytic leukaemia (PML) protein to organize the major histocompatibility complex (MHC) class I locus into distinct chromatin-loop architecture and mediate the expression of a subset of MHC class I genes. Upon Th2 cell activation, SATB1 expression is induced to compact chromatin into dense-loop structure and to prompt the expression of IL-4, IL-5, IL-13 and c-Maf. SATB1 is also reported as being crucial for the growth and metastasis of breast cancer and is a potential prognostic marker.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full length recombinant human SATB1 (NP_002962) produced in HEK293T cells.
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
Affinity purified.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, store undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 1.0 µg per ml. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Turovsky EA, et al. 2021. International Journal of Molecular Sciences. 22(11):. PubMed
RRID
AB_2565610 (BioLegend Cat. No. 676102)

Antigen Details

Structure
Homodimer. Canonical sequence contains 763 amino acids, predicted molecular weight of 86 kD. Isoform contains 795 amino acids, predicted molecular weight of 89 kD.
Distribution

Nucleus matrix. Nucleus. Promyelocytic leukemia nuclear bodies (SUMOylated).

Function
SATB1 recruits chromatin remodeling factors to regulate chromatin-loop structure and gene transcription. It is an important silencing factor for initiating the Xist RNA-mediated X inactivation in mammals.
Interaction
CUX1, CtBP1, PCAF, SUMOylated PML, HDAC1 and HIV-1 Tat, DYNLT2, POLR2J2, EP300, special AT-rich DNA sequences (consensus SATB1-binding sequence, CSBS).
Biology Area
Cell Biology, Immunology, Transcription Factors
Antigen References

1. Agrelo R, et al. 2009. Dev. Cell. 16:507.
2. Han HJ, et al. 2008. Nature. 452:187.
3. Cai S, et al. 2006. Nature Genet. 38:1278.
4. Pavan Kumar, P, et al. 2006. Mol. Cell. 22:231.
5. Fujii Y, et al. 2003. Microbiol. Immunol. 47:803.
6. Yasui, D, et al. 2002. Nature. 419:641.

Gene ID
6304 View all products for this Gene ID
UniProt
View information about SATB1 on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 06.10.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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