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Purified anti-SESN2 Antibody

Pricing & Availability
Clone
W18163A (See other available formats)
Regulatory Status
RUO
Other Names
Sestrin 2, Sestrin-2, SEST2, SES2
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W18163A_PURE_SESN2_Antibody_1_072720.png
Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1 µg/mL of purified anti-SESN2 antibody (clone W18163A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular weight marker.
  • W18163A_PURE_SESN2_Antibody_1_072720.png
    Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1 µg/mL of purified anti-SESN2 antibody (clone W18163A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular weight marker.
  • W18163A_PURE_SESN2_Antibody_2_072720.png
    Whole cell extracts (250 µg total protein) prepared from HeLa cells were immunoprecipitated overnight with 2.5 µg of purified rat IgG2a, κ isotype ctrl antibody (Cat. No. 400502) or purified anti-SESN2 antibody (clone W18163A). The resulting IP fractions and whole cell extract input (6%) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane and probed with the same purified anti-SESN2 antibody (overnight at 4°C). Proteins were visualized by chemiluminescence detection using an anti-rat HRP light-chain specific antibody. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution. Lane M: Molecular weight marker.
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937601 25 µg 81€
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937602 100 µg 203€
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Description

Sestrin 2 (SESN2) is part of the sestrin family of evolutionally conserved stress inducible proteins. SESN2 plays a role in cell growth and survival by protecting cells against oxidative and genotoxic stress. SESN2 reduces oxidative stress by activating Nrf2 and inhibits mTOR activation through AMPK. SESN2 is an intracellular leucine sensor which negatively regulates protein translation in response to ER stress by interacting with the TORC1 signaling pathway through GATOR2. SESN1 is a paralog of SESN2. Diseases associated with SESN2 include nodular ganglioneuroblastoma and pulmonary emphysema.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial recombinant human SESN2
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IP - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1 µg/mL. For immunoprecipitation, the suggested use of this reagent is 2.5 µg/test. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone recognizes human and mouse SESN2.

Clone does not work for ICC.

RRID
AB_2876754 (BioLegend Cat. No. 937601)
AB_2876754 (BioLegend Cat. No. 937602)

Antigen Details

Structure
SESN2 is a 480 amino acid protein with a predicted molecular weight of 54.5 kD. Western blot shows a band around 56 kD.
Distribution

SESN2 is primarily localized to the cytosol

Function
Gene expression, stress response
Interaction
GATOR2, AMPK, Nrf2
Antigen References
  1. Bodmer D and Soledad LH. 2017. Cell Death & Dis. 8(10):e3068.
  2. Kim MJ, et al. 2016. Autophagy. 8:1272.
  3. Zhao B, et al. 2017. Photochem Photobiol. 1:375-81
  4. Maiuri MC, et al. 2009. Cell Cyc. 10:1571.
Gene ID
83667 View all products for this Gene ID
UniProt
View information about SESN2 on UniProt.org
Go To Top Version: 1    Revision Date: 07.27.2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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