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Purified anti-STAT6 Antibody

Pricing & Availability
Clone
16G12A08 (See other available formats)
Regulatory Status
RUO
Other Names
Signal transducer and activator of transcription 6, IL-4 Stat
Isotype
Mouse IgG1, κ
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Product Citations
publications
a)16G12A08_PURE_STAT6_Antibody_WB_011618
Total lysates (15 µg protein) from HeLa (lane1), NTERA-2 (lane 2) and NIH3T3 (lane 3) cells were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 1:500 diluted (1 µg/mL) Purified anti-STAT6 Antibody, clone 16G12A08 (upper). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted anti-Mouse-IgG secondary antibody conjugated to HRP for the anti-STAT6 Antibody or 1:5000 diluted Direct-Blot™ HRP anti-β-Actin Antibody, clone 2F1-1(lower). Lane M: Molecular weight ladder.
  • a)16G12A08_PURE_STAT6_Antibody_WB_011618
    Total lysates (15 µg protein) from HeLa (lane1), NTERA-2 (lane 2) and NIH3T3 (lane 3) cells were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 1:500 diluted (1 µg/mL) Purified anti-STAT6 Antibody, clone 16G12A08 (upper). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted anti-Mouse-IgG secondary antibody conjugated to HRP for the anti-STAT6 Antibody or 1:5000 diluted Direct-Blot™ HRP anti-β-Actin Antibody, clone 2F1-1(lower). Lane M: Molecular weight ladder.
  • b)16G12A08_Purified_STAT6_Antibody_IF_111513
    Jurkat cells were stained with purified anti-STAT6 (clone 16G12A08) antibody, followed by staining with DyLight™ 488 conjugated goat anti-mouse IgG (green) antibody. Nuclei were stained with DAPI (blue).
  • c)16G12A08_Purified_STAT6_Antibody_IF_111513
    Immunoprecipitation of STAT6 from Jurkat cell extracts. Lane 1 is 5% input. Immunoprecipitation was performed using protein G resins only (lane 2), mouse IgG isotype control (lane 3), and anti-STAT6 antibody (clone 16G12A08, lane 4). Western blot was performed using anti-STAT6 antibody (clone 16G12A08). The astrostar indicates immunoglobulin heavy chain and light chain.
  • d)16G12A08_ChIP_STAT6_antibody_1_010818
    Chromatin Immunoprecipitation (ChIP) was performed using Go-ChIP-Grade™ Protein G Enzymatic Kit by loading 3 µg of cross-linked chromatin samples from Ramos cells starved overnight and then treated with IL-4 with either A) 1:50 dilution of Go-ChIP-Grade™ purified anti-STAT6 (clone 16G12A08), or B) equal amount of purified Mouse Ig1, κ isotype control antibody (Cat. No. 401401) or C) competitor’s ChIP-grade purified anti-STAT6 antibody and D) equal amount of matched isotype control antibody as recommended by the manufacturer. The enriched DNA was purified and quantified by real-time qPCR using primers targeting human DMD gene region. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the 5% of total amount of input chromatin.
Cat # Size Price Quantity Check Availability Save
657901 25 µg 95€
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657902 100 µg 212€
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Description

STAT6 is a member of the signal transducer and activator of transcription (STAT) family, activating gene expression in response to IL-4 and IL-13 stimulation. Upon cytokine stimulation, the receptor is phosphorylated by the associated Janus Kinases (Jak), followed by recruiting cytoplasmic STAT6. The Y641 residue of STAT6 is, in turn, phosphorylated by Jak. Phosphorylated STAT6 forms homodimers, transclocates to the nucleus, and regulates transcription of target genes. STAT6 plays crucial roles in differentiation of T helper 2 (Th2) cells, class switch of immunoglobulins in B cells, expression of cell surface markers such as MHC class II, and the development of allergic inflammation.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial human STAT6 recombinant protein (678-847 aa)
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
Affinity purified.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, store undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, IP, ChIP - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. For immunocytochemistry, a concentration of 5.0 μg/ml is recommended. For immunoprecipitation, the suggested use of this reagent is 5.0 - 20 μg per ml. For ChIP applications, the suggested use of this reagent is 1:50-1:100 by volume. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2562691 (BioLegend Cat. No. 657901)
AB_2562691 (BioLegend Cat. No. 657902)

Antigen Details

Structure
847 amino acids, predicted molecular weight of 94 kD. Contains a SH2 domain responsible for phosphor-Tyrosine binding and dimerization. The C-terminal LXXLL motif is required for interaction with NCOA1.
Distribution

Cytoplasm. Translocates to the nucleus when phosphorylated.

Function
STAT6 is a transcription factor, mediating IL-4 and IL-13 signaling.
Interaction
STAT6 interacts with CBP/p300 and NCOA1. Forms a homodimer or a heterodimer with other STAT family members.
Cell Type
B cells
Biology Area
Cell Biology, Immunology, Signal Transduction, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Goenka S, et al. 2011. Immunol. Res. 50:87.
2. Wurster AL, et al. 2000. Oncogene. 19:2577.
3. Akira S. 1999. Stem Cells 17:138.
4. Zamorano J, et al. 2005. J. Immunol. 174:2843.
5. David M, et al. 2001. Oncogene. 20:6660.
6. Takeda K, et al. 1996. Nature 380:627.

Gene ID
6778 View all products for this Gene ID 20852 View all products for this Gene ID
UniProt
View information about STAT6 on UniProt.org
Go To Top Version: 5    Revision Date: 08.24.2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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