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Purified anti-Transthyretin, aggregated Antibody

Pricing & Availability
Clone
2T5C9 (See other available formats)
Regulatory Status
RUO
Other Names
PALB, ATTR, Prealbumin, TBPA, TTR
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
2T5C9_PURE_Transthyretin_CAPTUREELISA_010317_resized
Capture ELISA of purified anti-Transthyretin, aggregated antibody (clone 2T5C9) binding to soluble aggregates of recombinant human TTR . ELISA was performed by coating wells with 100 ng of clone 2T5C9. The wells were then incubated at 37°C for 1h with serially diluted (0.005 to 10 µg/ml) soluble TTR aggregates, followed by incubation with horseradish peroxidase labeled goat anti-TTR secondary antibody. TMB (3, 3', 5, 5' tetramethylbenzidine) was used as the detection system (Cat. No. 421501).
  • 2T5C9_PURE_Transthyretin_CAPTUREELISA_010317_resized
    Capture ELISA of purified anti-Transthyretin, aggregated antibody (clone 2T5C9) binding to soluble aggregates of recombinant human TTR . ELISA was performed by coating wells with 100 ng of clone 2T5C9. The wells were then incubated at 37°C for 1h with serially diluted (0.005 to 10 µg/ml) soluble TTR aggregates, followed by incubation with horseradish peroxidase labeled goat anti-TTR secondary antibody. TMB (3, 3', 5, 5' tetramethylbenzidine) was used as the detection system (Cat. No. 421501).
  • 2T5C9_Purified_Transthyretin_Antibody_2_060419_updated.png
    Congo red (A) and purified anti-Transthyretin, aggregated antibody (clone 2T5C9) (B) staining of Transtheyretin amyloid deposits in formalin-fixed paraffin-embedded heart tissue of a patient with familial amyloidotic polyneuropathy (FAP). Following antigen retrieval using Antigen Retrieval Citra Plus (BioGenex), the tissue was incubated with the primary antibody at 3 µg/mL overnight at 4°C. Horse radish peroxidase conjugated anti-mouse IgG was used as the detection system.
See high resolution IHC-P data...
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848201 25 µg 90€
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848202 100 µg 231€
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Description

Transthyretin (TTR) is a carrier protein capable of transporting thyroid hormones in the plasma and cerebrospinal fluid, and retinol (vitamin A) in the plasma. TTR can form aberrant, insoluble amyloid fibrils that primarily deposit in a patient's heart, peripheral nerves, or both. The wild-type protein is associated with senile systemic amyloidosis (SSA) and is typically diagnosed in men over 70-years-old. Single or double mutations in TTR are primarily associated with familial amyloidotic polyneuropathy (FAP) or familial amyloid cardiopathy (FAC) which affects younger patients, often in their 30s. Clinical symptoms of TTR amyloidosis have historically been attributed to fibrils, but recent experimental evidence indicates that soluble TTR aggregates are also pathogenic.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
A 1:1 mixture of wild-type Transthyretin fibrils and soluble aggregates of Y78F TTR.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA Capture - Quality tested
IHC-P - Verified
Direct ELISA - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration of 2.0 µg/ml is recommended. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, a concentration of is 1.0 - 5.0 µg/ml is recommmended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone 2T5C9 recognizes a mid-region epitope on transthyretin aggregates.

Application References

(PubMed link indicates BioLegend citation)
  1. Phay M, et al. 2014. Rejuvenation Res. 17(2):97. (Direct ELISA, Capture ELISA, IHC) PubMed
RRID
AB_2632817 (BioLegend Cat. No. 848201)
AB_2632817 (BioLegend Cat. No. 848202)

Antigen Details

Structure
Transthyretin (TTR) is a β-sheet rich homotetrameric protein that has a molecular mass of 55 kD. Each monomer consists of 127 amino acids.
Distribution

Primarily extracellular in blood and cerebrospinal fluid.

Function
The normal function of TTR is to transport thyroxine and retinol-binding protein in the blood and cerebrospinal fluid.
Interaction
Perlecan, receptor for advanced glycation end product (RAGE).
Ligand/Receptor
Thyroxine, retinol-binding protein bound to retinol.
Cell Sources
Liver, choroid plexus epithelium, retina pigment epithelium.
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Antigen References

1. Plante-Bordeneuve V, Said G. 2011. Lancet Neurol. 10:1086. PubMed
2. Pinney JH, et al. 2013. J. Am. Heart Assoc. 2:e000098. PubMed
3. Hawkins PN, et al. 2015. Ann Med. 47(8):625. PubMed

Gene ID
7276 View all products for this Gene ID
UniProt
View information about Transthyretin on UniProt.org
Go To Top Version: 2    Revision Date: 06.04.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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