Purified anti-Tyrosine Hydroxylase Antibody (Previously Covance catalog# MMS-5210, MMS-5210-100, MMS5210)

Pricing & Availability
Clone
2/40/15 (See other available formats)
Regulatory Status
RUO
Other Names
Tyrosine 3-monooxygenase, tyrosine 3-hydroxylase
Previously
Covance Catalog# MMS-5210, MMS-5210-100, MMS5210
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
24015_pure_Tyrosine-Hydroxylase_Antibody_1_030618
IHC staining of purified anti-Tyrosine Hydroxylase antibody (clone 2/40/15) on formalin-fixed paraffin-embedded mouse (A) and (B) rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissues were incubated with 1 µg/ml of the primary antibody for 60 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The images were captured with a 40X objective. Scale bar: 50 µm
  • 24015_pure_Tyrosine-Hydroxylase_Antibody_1_030618
    IHC staining of purified anti-Tyrosine Hydroxylase antibody (clone 2/40/15) on formalin-fixed paraffin-embedded mouse (A) and (B) rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissues were incubated with 1 µg/ml of the primary antibody for 60 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The images were captured with a 40X objective. Scale bar: 50 µm
  • 24015_pure_Tyrosine-Hydroxylase_Antibody_2_030618
    IHC staining of purified anti-Tyrosine Hydroxylase antibody (clone 2/40/15) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/ml of the primary antibody for 60 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 24015_pure_Tyrosine-Hydroxylase_Antibody_3_030618
    IHC staining of purified anti-Tyrosine Hydroxylase antibody (clone 2/40/15) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/ml of the primary antibody for 60 minutes at room temperature followed by incubation with Alexa Fluor® 594 goat anti-mouse IgG for one hour at room temperature. The image was captured with a 40X objective. Scale Bar: 50 µm
  • 24015_pure_Tyrosine-Hydroxylase_Antibody_4_030618
    IHC staining of purified anti-Tyrosine Hydroxylase antibody (clone 2/40/15) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/ml of the primary antibody for 60 minutes at room temperature followed by incubation with Alexa Fluor® 594 goat anti-mouse IgG for one hour at room temperature. The image was captured with a 40X objective. Scale Bar: 50 µm
  • 24015_pure_Tyrosine-Hydroxylase_Antibody_5_030618
    IHC staining of purified anti-Tyrosine Hydroxylase antibody (clone 2/40/15) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/ml of the primary antibody for 60 minutes at room temperature followed by incubation with Alexa Fluor® 594 goat anti-mouse IgG for one hour at room temperature. The image was captured with a 40X objective. Scale Bar: 50 µm
  • 24015_pure_Tyrosine-Hydroxylase_Antibody_6_030618
    Western blot of purified anti-Tyrosine Hydroxylase antibody (clone 2/40/15). Lane 1: Molecular weight marker; Lane 2: 30 µg of mouse brain lysate; Lane 3: 30 µg of rat brain lysate. The blot was incubated with 1 µg/mL of the primary antibodies overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
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818002 25 µL 85€
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818001 100 µL 220€
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Description

Tyrosine hydroxylase, also known as tyrosine 3-monooxygenase, is the enzyme that converts amino acid L-Tyrosine to L-3, 4-Dihydroxyphenylalanine (L-DOPA).  L-DOPA is a precursor to dopamine, which is then converted to norepinephrine and epinephrine (adrenaline).  Dopamine, norepinephrine and epinephrine are all important neurotransmitters.  Alterations in the activity of tyrosine hydroxylase may be involved in a number of neurological diseases such as Parkinson's and schizophrenia.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against purified Tyrosine Hydroxylase from rat Pheochromocytoma tumor.
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application

IHC-P - Quality tested
WB - Verified

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 0.5 - 1.0 µg/ml is suggested. For Western blotting, the suggested use of this reagent is 1.0 - 10.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in immunoblotting (WB) and formalin-fixed paraffin-embedded immunohistochemical staining (IHC-P).

This antibody is less reactive in human tissue in Western Blot.

Product Citations
  1. Harraz MM, et al. 2022. Proc Natl Acad Sci U S A. 119:e2200545119. PubMed
  2. Harraz MM, et al. 2021. Mol Psychiatry. 26:370. PubMed
  3. Shi L, et al. 2021. Translational Neurodegeneration. 10(1):13. PubMed
  4. Marfull-Orom P, et al. 2021. Journal of Neurochemistry. . PubMed
RRID
AB_2564816 (BioLegend Cat. No. 818002)
AB_2564816 (BioLegend Cat. No. 818001)

Antigen Details

Structure
Expected MW: 65 kD
Cell Type
Dopaminergic Neurons, Neural Stem Cells, Neurons
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers, Stem Cells, Synaptic Biology
Molecular Family
Enzymes and Regulators, Presynaptic proteins
Gene ID
25085 View all products for this Gene ID
UniProt
View information about Tyrosine Hydroxylase on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 5    Revision Date: 07.09.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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