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Purified anti-UBE2O Antibody

Pricing & Availability
Clone
A18140A (See other available formats)
Regulatory Status
RUO
Other Names
(E3-independent) E2 ubiquitin-conjugating enzyme, E2/E3 hybrid ubiquitin-protein ligase UBE2O, Ubiquitin carrier protein O, Ubiquitin-conjugating enzyme E2 O, Ubiquitin-conjugating enzyme E2 of 230 kD, Ubiquitin-protein ligase O
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A18140A_PURE_UBE20_Antibody_1_120219
Western blot of purified anti-UBE2O antibody (clone A18140A). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 0.2 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP goat anti-mouse IgG antibody (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • A18140A_PURE_UBE20_Antibody_1_120219
    Western blot of purified anti-UBE2O antibody (clone A18140A). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 0.2 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP goat anti-mouse IgG antibody (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • A18140A_PURE_UBE20_Antibody_2_120219
    ICC staining of purified anti-UBE2O antibody (clone A18140A) on A431 cells. The cells were fixed with 4% PFA, permeabilized with a buffer containing 0.1% Triton X-100 and 0.25% BSA, and blocked with 2% normal goat serum and 0.02% BSA. The cells were then incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with 2.5 µg/mL of Alexa Fluor® 594 goat anti-mouse IgG antibody (Cat. No. 405326) for one hour at room temperature. The cells were co-stained with Flash Phalloidin™ Green 488 (Cat. No. 424201). Nuclei were counterstained with DAPI. The image was captured with a 60X objective. Scale bar: 20 µm
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870201 25 µg 85€
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870202 100 µg 221€
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Description

Ubiquitin-conjugating enzyme E2 O (UBE2O) is an E2/E3 hybrid enzyme that displays both E2 and E3 ligases activities and mediates ubiquitination of many target proteins involved in a wide range of processes, including the 5’-AMP-activated protein kinase catalytic subunit a2 (AMPKa2), tumor suppressor ubiquitin carboxyl-terminal hydrolase BAP1, mixed-lineage leukemia (MLL) protein, SMAD family member 6 (SMAD6), transcription factor c-Maf, and free ribosomal proteins. Abnormal expression and mutations in UBE2O are observed in tumorigenesis and are associated with poor prognosis of gastric, lung, breast, and prostate cancers.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human UBE2O protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.2 - 5.0 µg/mL. For immunocytochemistry, a concentration range of 0.5 - 10 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2820188 (BioLegend Cat. No. 870201)
AB_2820188 (BioLegend Cat. No. 870202)

Antigen Details

Structure
Human UBE2O is a 1,292 amino acid protein with a predicted molecular mass of 141 kD and observed molecular mass of ~200 kD
Distribution

Tissue distribution: Brain, skeletal muscle, heart
Cellular distribution: Nucleus, cytosol

Function
UBE2O is an E2/E3 hybrid ubiquitin-protein ligase that is crucial for protein ubiquitination and degradation.
Cell Type
Astrocytes, Microglia, Neurons, Oligodendrocytes
Biology Area
Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation, Ubiquitin/Protein Degradation
Molecular Family
Ligases
Antigen References
  1. Ullah K, et al. 2019. FEBS J. 286(11):2018-2034.
  2. Chen S, et al. 2018. J Biol Chem. 293(29):11296-11309.
  3. Mashtalir N, et al. 2014. Mol Cell. 54(3):392-406.
Gene ID
63893 View all products for this Gene ID
UniProt
View information about UBE2O on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 12.02.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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