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Purified anti-Ubiquitin Antibody

Pricing & Availability
Clone
P4G7 (See other available formats)
Regulatory Status
RUO
Other Names
Polyubiquitin-C, Polyubiquitin B, RPS 27A, RPS27A, UBA 52, UBA 80, UBA52, UBA80, UBB, UBC, UBCEP 1, UBCEP 2, UBCEP1, UBCEP2, Ubiquitin, ubiquitin B
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
1_P4G7_PURE_Ubiquitin_Antibody_2_030719.png
Western blot of purified anti-Ubiquitin antibody (clone P4G7). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain total lysate; Lane 3: 20 µg of mouse brain total lysate; Lane 4: 20 µg of rat brain total lysate. The blot was incubated with 5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 1_P4G7_PURE_Ubiquitin_Antibody_2_030719.png
    Western blot of purified anti-Ubiquitin antibody (clone P4G7). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain total lysate; Lane 3: 20 µg of mouse brain total lysate; Lane 4: 20 µg of rat brain total lysate. The blot was incubated with 5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 1_P4G7_PURE_Ubiquitin_Antibody_3_030719.png
    Western blot of purified anti-Ubiquitin antibody (clone P4G7). Lane 1: Molecular weight marker; Lane 2: 20 µg of HepG2 cell lysate; Lane 3: 20 µg of NIH3T3 cell lysate. The blot was incubated with 5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 1_P4G7_PURE_Ubiquitin_Antibody_4_030719.png
    Western blot of purified anti-Ubiquitin antibody (clone P4G7). Lane 1: Molecular weight marker; Lane 2: 20 µg of Drosophila head lysate; Lane 3: 20 µg of Drosophila S2 cell lysate. The blot was incubated with 2.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 2-P4G7_PURE_Ubiquitin_Antibody_IHC-P_1_030519
    IHC staining of purified anti-Ubiquitin antibody (clone P4G7) on formalin-fixed paraffin-embedded AD brain tissue. Following antigen retrieval using using Sodium Citrate H.I.E.R, the tissue was incubated with 5 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm.
  • 3-P4G7_PURE_Ubiquitin_Antibody_IHC-P_2_030519
    IHC staining of purified anti-Ubiquitin antibody (clone P4G7) on formalin-fixed paraffin-embedded AD brain tissue. Following antigen retrieval using using Sodium Citrate H.I.E.R, the tissue was incubated with 5 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm.
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838703 25 µg 90€
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838704 100 µg 221€
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Description

Ubiquitin is a small (8.5 kD) regulatory protein that is ubiquitously expressed in tissues of eukaryotic organisms. There are four genes in the human genome that produce ubiquitin; UBB, UBC, UBA52 and RPS27A. UBA52 and RPS27A genes code for a single copy of ubiquitin fused to the ribosomal proteins L40 and S27a, respectively. The UBB and UBC genes code for polyubiquitin precursor proteins.

Ubiquitination is a post-translational modification where a ubiquitin subunit is attached to a protein. Addition of ubiquitin can signal for degradation via the proteasome, alter cellular location, promote or prevent protein interactions, or affect activity. Ubiquitination is carried out stepwise by ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s), and ubiquitin ligases (E3s), respectively. The cascade results in the binding of ubiquitin to lysine residues on the protein substrate via an isopeptide bond, cysteine residues through a thioester bond, serine and threonine residues through an ester bond, or the amino group of the protein's N-terminus via a peptide bond.

Proteins can be modified either by a single ubiquitin unit (monoubiquitination) or a chain of ubiquitin molecules (polyubiquitination). Proteins destined for degradation by the proteasome are usually ubiquitinated on lysine residues K48 and K29, while other polyubiquitinations (e.g. on K63, K11, K6) and monoubiquitinations may regulate processes such as endocytic trafficking, inflammation, translation and DNA repair.

A frameshift mutation in ubiquitin B can result in a truncated peptide missing the C-terminal glycine. This abnormal peptide, known as UBB+1, has been shown to accumulate selectively in Alzheimer's disease and other tauopathies.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse, Rat, Drosophila
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Clone P4G7 was raised against denatured bovine ubiquitin and recognizes ubiquitin, polyubiquitin, and ubiquitin-conjugated proteins.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 10 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue, a concentration range of 0.5 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

P4G7 is equivalent to MAb P4D1. The ubiquitin protein is extremely well conserved and thus the antibody has extensive species cross-reactivity from yeast to human. This antibody was specifically developed to detect ubiquitin and ubiquitin-substrate conjugates by immunoblotting.

Application References

(PubMed link indicates BioLegend citation)
  1. Duttler S, et al. 2013. Mol. Cell. 3:379. (WB) PubMed
  2. Wu Y, et al. 2007. Mol. Cell. Biol. 18:6407. (WB) PubMed
Product Citations
  1. Weskamp G, et al. 2020. J Biol Chem. 295:4350. PubMed
  2. Lora J, et al. 2021. J Biol Chem. :100733. PubMed
  3. Kumar P, et al. 2021. Journal of Cellular Biochemistry. 122:1737. PubMed
  4. Quiñones JL, et al. 2020. DNA Repair (Amst). 102773:87. PubMed
  5. Weskamp G, et al. 2020. J Biol Chem. 295:4350. PubMed
RRID
AB_2632643 (BioLegend Cat. No. 838703)
AB_2632643 (BioLegend Cat. No. 838704)

Antigen Details

Structure
Ubiquitin is a 76 amino acid protein with a molecular mass of 8.5 kD.
Distribution

Found in almost all tissues of eukaryotic organisms

Function
Regulatory protein
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Neuroscience Cell Markers, Protein Trafficking and Clearance, Signal Transduction
Molecular Family
Autophagosome Markers
Antigen References

1. Hatanaka H, et al. 2009. J. Biol. Chem. 23:15448. (WB) PubMed
2. Sarkari F, et al. 2013. J. Biol. Chem. 23:16975. (WB) PubMed

Gene ID
7316 View all products for this Gene ID
UniProt
View information about Ubiquitin on UniProt.org
Go To Top Version: 2    Revision Date: 03.07.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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