IMPORTANT NOTICE: Our email addresses will soon change. Learn more >>
Loading...

RBC Lysis Buffer (10X)

Pricing & Availability
Regulatory Status
RUO
Other Names
Red blood cell lysis buffer
Ave. Rating
Submit a Review
Product Citations
publications
Cat # Size Price Quantity Check Availability Save
420301 100 mL 52€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
420302 500 mL 165€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

Red Blood Cell (RBC) Lysis Buffer has been designed, formulated, and tested to ensure optimal lysis of RBCs in single cell suspensions with minimal effects on leukocytes. RBC Lysis Buffer is supplied as a 10X solution containing ammonium chloride, potassium carbonate, and EDTA, and should be diluted in deionized water prior to use. Nucleated RBCs are not effectively lysed with ammonium chloride. Test

Product Details
Technical data sheet

Product Details

Formulation
This red blood cell (RBC) lysis buffer is supplied as a 10X solution and should be diluted to 1X in deionized water. 100 ml of 10X concentrate will yield a quantity of 1X solution that is sufficient to lyse 500 samples.
Preparation
The pH of the 1X solution should fall within the range of pH 7.1-7.4. Adjust the pH if necessary. Warm the 1X solution to room temperature prior to use.
Storage & Handling
Store RBC lysis buffer between 2°C and 8°C.
Application Notes

A. Lysis of Mouse Spleen RBCs:
1. Harvest mouse spleen and prepare a single cell suspension.
2. Pellet the cells by centrifugation (350 x g); aspirate the supernatant.
3. Dilute the 10X Red Blood Cell Lysis Buffer to 1X working concentration with deionized water and resuspend the pellet in 5 ml of 1X Lysis Buffer.
4. Incubate on ice for 4-5 minutes with occasional shaking.
5. Stop the reaction by diluting the Lysis Buffer with 20-30 ml of 1X PBS.
6. Spin the cells (350 x g) and discard the supernatant.
7. Resuspend the pellet in the appropriate buffer (e.g., BioLegend Cell Staining Buffer Cat. No. 420201), wash 1X.
8. Count cells, adjust density, and proceed with cell staining procedures.
B. Lysis of Human Peripheral Blood RBCs:
1. Dilute the 10X RBC Lysis Buffer to 1X working concentration with deionized water. Warm the 1X solution to room temperature prior to use.
2. Add 2.0 ml of 1X RBC Lysis Buffer to each tube containing up to 100 µl of whole blood.
3. Gently vortex each tube immediately after adding the lysing solution. Incubate at room temperature, protected from light, for 10-15 minutes.
4. Centrifuge 350 x g for 5 minutes. Aspirate supernatant without disturbing pellet.
5. Resuspend the pellet in the appropriate buffer (e.g., BioLegend Cell Staining Buffer, Cat. No. 420201), wash 1X.
6. Resuspend and proceed with further procedures.

Application References

(PubMed link indicates BioLegend citation)
  1. Adams RA, et al. 2007. J. Exp. Med. 204:571.
  2. Feild-Corbett C, et al. 2009. Stem Cells. PubMed
  3. de Resende MM, et al. 2010. Physiol Genomics. 42:437. PubMed
  4. Roybal JD, et al. 2011. Mol Cancer Res. 9:25. PubMed
  5. Boldin MP, et al. 2011. J Exp Med. PubMed
  6. Kahlenberg JM, et al. 2011. J. Immunol. 187:6143. PubMed
  7. Deiuliis JA, et al. 2012. Am J Physiol Lung Cell Mol Physiol. 302:399. PubMed
  8. Wu CY, et al. 2012. PLoS One. 7:e42365. PubMed
  9. Hsia BJ, et al. 2012. J Allergy Clin Immunol. 130:205. PubMed
  10. Rico C, et al. 2012. Carcinogenesis. 33:2283. PubMed
  11. Jin J, et al. 2012. Arterioscler Thromb Vasc Biol. 32:2901. PubMed
  12. Saraiva AM, 2013. Hum Immunol. 74:207. PubMed
  13. Koon HW, et al. 2013. J Vis exp. 68:4208. PubMed
  14. Ma Dy, et al. 2013. J Virol. 87:3361. PubMed
  15. Han W, 2013. J. Immunol. 190:4786. PubMed
  16. Garcia-Acros I, et al. 2013. J Biol Chem. 288:14046. PubMed
  17. Zhao JL, et al. 2013. Elife. 21:537. PubMed
  18. Guo W, et al. 2013. J Gerontol A Biol Sci Med Sci. PubMed
  19. Yamasaki S, et al. 2013. Clin Vaccine immunol. 20:1508. PubMed
  20. Kraft P, et al. 2013. Stroke. 44:3202. PubMed
  21. Liu X, et al. 2013. Invest Ophthalmol Vis Sci. 54:7386. PubMed
  22. Fullerton AM, et al. 2013. Toxicol Sci. 136:72. PubMed
  23. Cekic C, et al. 2013. J Exp Med. 210:2693. PubMed
  24. Varga T, et al. 2013. J. Immunol. 191:5695. PubMed
  25. Ma Y, et al. 2014. Cancer Res. 74:436. PubMed
  26. Chang YJ, et al. 2014. Cytotherapy. 16:560. PubMed
  27. Kim MJ, et al. 2014. Mol Pharmacol. 86:284. PubMed
  28. Cekic C, et al. 2014. Cancer Res. 74:7250. PubMed
  29. Szulzewsky F, et al. 2015. PloS One. 10:116644. PubMed
Product Citations
  1. Guennoun R, et al. 2022. Cancers (Basel). 14:. PubMed
  2. Zhuo Y, et al. 2022. J Immunother Cancer. 10:. PubMed
  3. Knelson EH, et al. 2022. Cancer Immunol Res. 10:947. PubMed
  4. Wakiyama H, et al. 2022. Cancer Immunol Res. 10:1386. PubMed
  5. Roweth HG, et al. 2022. Sci Adv. 8:eabo5224. PubMed
  6. Zhong J, et al. 2022. J Adv Res. 41:145. PubMed
  7. Yun HJ, et al. 2022. Int J Mol Sci. 23:. PubMed
  8. Daum P, et al. 2023. Front Immunol. 13:991347. PubMed
  9. Rao D, et al. 2023. Eur J Immunol. 53:e2250258. PubMed
  10. Le DT, et al. 2023. iScience. 26:106059. PubMed
  11. Romo-Rodríguez R, et al. 2023. Arch Med Res. 54:197. PubMed
  12. Mabire M, et al. 2023. Nat Commun. 14:1830. PubMed
  13. MacPherson KP, et al. 2023. Front Cell Neurosci. 17:895017. PubMed
  14. Chen Z, et al. 2023. Nat Commun. 14:1839. PubMed
  15. Martins Nascentes Melo L, et al. 2023. J Immunother Cancer. 11:. PubMed
  16. Ramalingam P, et al. 2023. Nat Commun. 14:2018. PubMed
  17. Choi YJ, et al. 2023. Nat Commun. 14:2045. PubMed
  18. He Q, et al. 2023. Parasit Vectors. 16:139. PubMed
  19. Pattnaik A, et al. 2023. Vaccines (Basel). 11:. PubMed
  20. Mulcrone PL, et al. 2023. Sci Rep. 13:8436. PubMed
  21. Villalba N, et al. 2023. J Neuroinflammation. 20:127. PubMed
  22. Song S, et al. 2023. Bioact Mater. 28:358. PubMed
  23. Matsudaira T, et al. 2023. Commun Biol. 6:665. PubMed
  24. Dobie R, et al. 2020. Cell Reports. 29(7):1832-1847.e8.. PubMed
  25. Strickley JD, et al. 2019. Nature. 575:519. PubMed
  26. Deiuliis J, et al. 2012. Am J Physiol Lung Cell Mol Physiol. 302:L399. PubMed
  27. Jin J, et al. 2012. Arterioscler Thromb Vasc Biol. 32:2901. . PubMed
  28. Barros-Martins J, et al. 2016. J Immunol. 196: 3642 - 3652. PubMed
  29. Zhou B, et al. 2020. Nat Immunol. 1.868055556. PubMed
  30. Tan J, et al. 2021. iScience. 24(8):102835. PubMed
  31. Asem M, et al. 2020. Cancer Res. 1156:80. PubMed
  32. Lin C, et al. 2020. Cancer Immunol Res. 632:8. PubMed
  33. Tsai AG, et al. 2020. Nat Med. 408:26. PubMed
  34. Shah D, et al. 2021. Oncoimmunology. 10:1939601. PubMed
  35. Hutton C, et al. 2021. Cancer Cell. 39:1227. PubMed
  36. Sandovici I, et al. 2022. Dev Cell. 57:63. PubMed
  37. Rathakrishnan A, et al. 2020. Viruses. 12: . PubMed
  38. Wang Y, et al. 2021. Cancer Res. 81:384. PubMed
  39. Wang H, et al. 2022. Nat Biomed Eng. 6:882. PubMed
  40. Yeh PY, et al. 2022. Sens Actuators B Chem. 353: . PubMed
  41. Ammothumkandy A, et al. 2022. Nat Neurosci. 25:493. PubMed
  42. Melendez E, et al. 2022. Development. 149: . PubMed
  43. Argueta LB, et al. 2022. iScience. 25:104223. PubMed
  44. Wang S, et al. 2022. Adv Sci (Weinh). 9:e2201258. PubMed
  45. Meena AS, et al. 2022. Cell Rep. 39:110937. PubMed
  46. Zhang Y, et al. 2022. Vaccines (Basel). 10: . PubMed
  47. Sugimoto S, et al. 2022. Nat Metab. 4:775. PubMed
  48. Moore AR, et al. 2022. Cell Rep. 41:111651. PubMed
  49. Isildar B, et al. 2022. Inflamm Regen. 42:55. PubMed
  50. Xu Q, et al. 2022. Front Med (Lausanne). 9:940554. PubMed
  51. Alexandrou AT, et al. 2022. iScience. 25:105546. PubMed
  52. Wang Y, et al. 2022. Front Endocrinol (Lausanne). 13:1036517. PubMed
  53. He Y, et al. 2022. Front Immunol. 13:986202. PubMed
  54. Tratwal J, et al. 2022. Front Endocrinol (Lausanne). 13:1001210. PubMed
  55. Xie Z, et al. 2022. Cell Discov. 8:133. PubMed
  56. Xiang X, et al. 2022. Medicine (Baltimore). 101:e32558. PubMed
  57. Yu Z, et al. 2023. Cancer Res. 83:700. PubMed
  58. Ding D, et al. 2023. J Med Chem. 66:1467. PubMed
  59. Oh S, et al. 2023. Nat Biotechnol. :. PubMed
  60. Marritt KL, et al. 2023. Front Immunol. 13:1087991. PubMed
  61. He T, et al. 2023. Immun Inflamm Dis. 11:e759. PubMed
  62. Vyas M, et al. 2023. Front Immunol. 13:1098445. PubMed
  63. Li XF, et al. 2023. J Exp Med. 220: . PubMed
  64. Wang J, et al. 2023. Inflamm Regen. 43:12. PubMed
  65. Ma Y, et al. 2022. Am J Physiol Heart Circ Physiol. 322:H622. PubMed
  66. Feng J, et al. 2022. Immunity. 55:405. PubMed
  67. Rustagi Y, et al. 2022. Diabetes. 71:1149. PubMed
  68. Herisson F, et al. 2018. Nat Neurosci. 21:1209. PubMed
  69. Shin J, et al. 2020. Hum Gene Ther. . PubMed
  70. Bouchareychas L, et al. 2021. iScience. 24(8):102847. PubMed
  71. Spengler J, et al. 2016. J Infect Dis. 214: S308 - S318. PubMed
  72. Zheng M, et al. 2016. J Immunol. 196: 4122 - 4131. PubMed
  73. St-Pierre S, et al. 2016. PLoS One. 11: 0150230. PubMed
  74. Ensan S, et al. 2015. Nat Immunol. 17: 159-168. PubMed
  75. Jaiswal M, et al. 2015. Sci Rep. 5: 15221. PubMed
  76. Hou J, et al. 2015. J Mol Cell Biol. 7: 338-350. PubMed
  77. Fujii H, et al. 2015. PLoS One. 10: 0133216. PubMed
  78. Pracht K, et al. 2021. Eur J Immunol. 51:1089. PubMed
  79. Radwanska M, et al. 2020. Methods Mol Biol. 2116:721. PubMed
  80. Rendon CJ, et al. 2022. Front Endocrinol (Lausanne). 13:995499. PubMed
  81. Effern M, et al. 2022. STAR Protoc. 3:101038. PubMed
  82. Della Verde G, et al. 2022. Life Sci Alliance. 5:. PubMed
  83. Su C, et al. 2021. Front Oncol. 11:719564. PubMed
  84. Jacoby E, et al. 2021. NPJ Regen Med. 6:58. PubMed
  85. Chen Z, et al. 2020. Glia. 68:2148. PubMed
  86. Seow JJW, et al. 2021. Front Med (Lausanne). 8:603374. PubMed
  87. Liu W, et al. 2017. Int J Environ Res Public Health. 10.3390/ijerph14060592. PubMed
  88. Pietrocola F, et al. 2017. Autophagy. 0.935416667. PubMed
  89. Chou YJ, et al. 2020. Sci Rep. 10:8422. PubMed
  90. Wang J, et al. 2020. Sci Rep. 10:12785. PubMed
  91. Ma Y, et al. 2014. Cancer Res. 74:436. PubMed
  92. Magalhães L, et al. 2015. PLoS Negl Trop Dis. 10: 0003816. PubMed
  93. Moraes-Vieira P, et al. 2016. Diabetes. 65: 1317 - 1327. PubMed
  94. Garcia-Fabiani MB, et al. 2020. STAR Protoc. :1. PubMed
  95. Zhang J, et al. 2021. Aging (Albany NY). 13:17880. PubMed
  96. Press AT, et al. 2021. EMBO Mol Med. 13:e14436. PubMed
  97. Sima LE, et al. 2021. J Immunother Cancer. 9:. PubMed
  98. Ma H, et al. 2021. Front Immunol. 12:760095. PubMed
  99. McGill CJ, et al. 2021. STAR Protoc. 2:100948. PubMed
  100. Lutze J, et al. 2022. Methods Mol Biol. 2394:299. PubMed
  101. Hosseini A, et al. 2022. Evid Based Complement Alternat Med. 2022:5639226. PubMed
  102. Bikorimana JP, et al. 2022. Cell Rep Med. 3:100534. PubMed
  103. Zhang L, et al. 2022. JCI Insight. 7:. PubMed
  104. Baldominos P, et al. 2022. STAR Protoc. 3:101795. PubMed
  105. Boldin M, et al. 2011. J Exp Med. 208:1189. PubMed
  106. Sun CC, et al. 2020. Genome Med. 0.553472222. PubMed
  107. Bravo–San Pedro JM, et al. 2019. Cell Metab. 30:754. PubMed
  108. Clark EL, et al. 2017. PLoS Genet. 13:e1006997. PubMed
  109. Cantor JR, et al. 2017. Cell. 169:258. PubMed
  110. Ji WJ, et al. 2017. Cytometry B Clin Cytom. 92:371. PubMed
  111. Devalaraja S, et al. 2020. Cell. 1098:180. PubMed
  112. Elisabeth Schrumpf, Xiaojun Jiang 2017. Physiol Rep. 10.14814/phy2.13117. PubMed
  113. AC Belkina, JE Snyder-Cappione 2017. Cytometry A. 91:175-179. PubMed
  114. Brunmeir R, et al. 2016. PLoS Genet. 12:e1006474. PubMed
  115. Pridans C, et al. 2016. J Immunol. 197: 2297 - 2305. PubMed
  116. Mircheff A, et al. 2016. Ocul Surf. 14:460-483. PubMed
  117. Pennington L, et al. 2016. Nat Commun. 7:11610. PubMed
  118. Koon H, et al. 2012. J Vis Exp. 68: 4208. PubMed
  119. Chen J, et al. 2022. Nat Commun. 13:6759. PubMed
  120. Wu Y, et al. 2021. Nat Biomed Eng. 5:1336. PubMed
  121. Meng S, et al. 2022. Exp Anim. . PubMed
  122. Kokkinopoulos I, et al. 2021. Sci Rep. 11:15759. PubMed
  123. Johnson CL, et al. 2021. Am J Physiol Heart Circ Physiol. 321:H756. PubMed
  124. Stringhini M, et al. 2021. MAbs. 13:1868066. PubMed
  125. Sakamoto S, et al. 2020. OncoImmunology. 8(12):e1671760.. PubMed
  126. Rico C, et al. 2012. Carcinogenesis. 33:2283. PubMed
  127. Hamour S, et al. 2015. PLoS One. 10: 0136238. PubMed
  128. Bazett M, Haston M 2016. Sci Rep. 6:19189. PubMed
  129. Luo B, et al. 2016. J Neurosci. 36: 9590 - 9603. PubMed
  130. Patera A, et al. 2016. J Leukoc Biol. 100(6):1239-1254. PubMed
  131. Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed
  132. Zhai Z, et al. 2021. Medicine (Baltimore). 100:e26214. PubMed
  133. Hu D, et al. 2021. J Cell Mol Med. 25:2900. PubMed
  134. Jones N, et al. 2021. Nat Commun. 12:1209. PubMed
  135. Wang D, et al. 2021. Signal Transduct Target Ther. 6:376. PubMed
  136. Machado H, et al. 2021. PLoS Pathog. 17:e1009933. PubMed
  137. Yan X, et al. 2022. Int J Biol Sci. 18:585. PubMed
  138. Singh SS, et al. 2021. Eur J Med Chem. 216:113312. PubMed
  139. Pokhrel RH, et al. 2022. Int J Mol Sci. 23:. PubMed
  140. Vannini N, et al. 2019. Cell Stem Cell. 24:405. PubMed
  141. Jiang J, et al. 2018. Cancer Med. 3.504861111. PubMed
  142. Chen Z, et al. 2017. Cancer Res. 77:2266. PubMed
  143. Kim M, et al. 2017. Int J Mol Sci. 10.3390/ijms18061275. PubMed
  144. Facchini M, et al. 2014. J Vis Exp. 85: 51019. PubMed
  145. Ilievski V, et al. 2015. PLoS One. 10: 0136502. PubMed
  146. Saraiva A, et al. 2013. Hum Immunol. 74:207. PubMed
  147. Mo L, et al. 2022. Front Oncol. 12:871489. PubMed
  148. Huang L, et al. 2021. J Cell Physiol. 236:7308. PubMed
  149. Whytock KL, et al. 2022. iScience. 25:104772. PubMed
  150. Neufeld L, et al. 2021. Sci Adv. 7:. PubMed
  151. Friedman G, et al. 2020. Nat Cancer. 1:692. PubMed
  152. Kumar A, et al. 2021. Part Fibre Toxicol. 18:43. PubMed
  153. Tanaka Y, et al. 2021. Int J Mol Sci. 22:. PubMed
  154. Scherer SD, et al. 2021. Breast Cancer Res. 23:100. PubMed
  155. Jeong J, et al. 2021. Immune Netw. 21:e31. PubMed
  156. Sitaraman S, et al. 2021. JCI Insight. 6:. PubMed
  157. Orr AN, et al. 2021. Int J Mol Sci. 22:. PubMed
  158. Wang K, et al. 2017. Molecular Immunology. 10.1016/j.molimm.2017.04.016. PubMed
  159. Appelbe O, et al. 2017. J Control Release.. 10.1016/j.jconrel.2017.09.043. PubMed
  160. Tang J, et al. 2017. Proc Natl Acad Sci U S A. 114:E7441. PubMed
  161. Liccardi G, et al. 2019. Mol Cell. 73:413. PubMed
  162. Patil R, et al. 2019. J Biomed Opt. 24:1. PubMed
  163. Ongaro T, et al. 2020. Oncotarget. 3.026388889. PubMed
  164. Kahlenberg J, et al. 2011. J Immunol. 187:6143. PubMed
  165. Han W, et al. 2013. J Immunol. 190:4786. PubMed
  166. Yamasaki S, et al. 2013. Clin Vaccine Immunol. 20:1508. PubMed
  167. Fukui R, et al. 2016. J Immunol. 196: 416 - 427. PubMed
  168. Rodriguez-Garcia M, et al. 2016. Mucosal Immunol. 10.1038/mi.2016.72. PubMed
  169. Kildisiute G, et al. 2021. Sci Adv. 7: . PubMed
  170. Schiferle EB, et al. 2021. Sci Adv. 7: . PubMed
  171. Devalaraja S, et al. 2020. STAR Protoc. 1:100188. PubMed
  172. Bigenwald C, et al. 2021. Nat Med. 27:851. PubMed
  173. Gingrich AA, et al. 2021. Front Immunol. 12:670309. PubMed
  174. Dang Y, et al. 2022. Cancer Cell Int. 22:88. PubMed
  175. Deng J, et al. 2021. Breast Cancer Res Treat. 185:85. PubMed
  176. Vivanco Gonzalez N, et al. 2022. STAR Protoc. 3:101280. PubMed
  177. Qin L, et al. 2022. Nat Commun. 13:6051. PubMed
  178. Escudero-Lara A, et al. 2022. Front Behav Neurosci. 16:946975. PubMed
  179. Martínez-Muriana A, et al. 2020. Stem Cell Res Ther. 11:53. PubMed
  180. Pennig J, et al. 2019. Sci Rep. 9:17937. PubMed
  181. Wang B, et al. 2017. Stem Cell Res Ther.. 10.1186/s13287-017-0744-6. PubMed
  182. Anstine L,et al. 2017. J Am Heart Assoc.. 10.1161/JAHA.117.007097. PubMed
  183. Judge SJ, et al. 2020. J Clin Invest. 130:3051. PubMed
  184. Choi JW, et al. 2020. PLoS One. 15:e0235518. PubMed
  185. Alban TJ, et al. 2020. Front Immunol. 1.285416667. PubMed
  186. Jiang S, et al. 2017. Toxicology Lett. 10.1016/j.toxlet.2017.03.015. PubMed
  187. Agrawal V, et al. 2016. J Leukoc Biol. 100: 261 - 274. PubMed
  188. Zhang L, et al. 2021. Methods Mol Biol. 2388:175. PubMed
  189. Evans AK, et al. 2020. Neurobiol Dis. 146:105089. PubMed
  190. Elphinstone R, et al. 2017. Infect Immun. 10.1128/IAI.00371-17. PubMed
  191. Kernfeld EM, et al. 2018. Immunity. 48:1258. PubMed
  192. Nwani NG, et al. 2019. Cancers (Basel). 0.806944444. PubMed
  193. Ramalingam P, et al. 2020. Nat Commun. 11:666. PubMed
  194. Garcia-Arcos I, et al. 2013. J Biol Chem. 288:14046. PubMed
  195. Sci T 2013. Toxicol Sci. 136:72. PubMed
  196. Metz H, et al. 2016. Proc Natl Acad Sci U S A. 113: 8795 - 8800. PubMed
  197. Cheng Q, et al. 2020. Nat Nanotechnol. 0.842361111. PubMed
  198. Xie Z, et al. 2020. Light Sci Appl. 0.486805556. PubMed
  199. Alghamri MS, et al. 2020. Methods Enzymol. 632:215. PubMed
  200. Yu Z, et al. 2021. Front Oncol. 11:659251. PubMed
  201. Deng J, et al. 2021. Nat Cancer. 2:503. PubMed
  202. Beaudoin-Bussières G, et al. 2022. Cell Rep. 38:110368. PubMed
  203. Hu H, et al. 2021. Cancer Immunol Res. 9:1298. PubMed
  204. Zhong W, et al. 2022. Nat Commun. 13:4390. PubMed
  205. So EY, et al. 2021. Am J Physiol Cell Physiol. 321:C569. PubMed
  206. Bunting MD, et al. 2022. Sci Adv. 8:eabk3327. PubMed
  207. Mirando AC, et al. 2020. Oncoimmunology. 9:1760685. PubMed
  208. Rosenbluth JM, et al. 2020. Nat Commun. 11:1711. PubMed
  209. Ramaswamy G, et al. 2017. Sci Rep. 10.1038/srep45140. PubMed
  210. Lizotte P, et al. 2016. Sci Rep. 6:31745. PubMed
  211. Tavakol DN, et al. 2021. Curr Protoc. 1:e275. PubMed
  212. Ullah I, et al. 2021. Immunity. 54:2143. PubMed
  213. He Y, et al. 2021. Front Immunol. 12:641206. PubMed
  214. , et al. 2021. Eur J Immunol. 51:2708. PubMed
  215. Ercoli G, et al. 2022. Clin Transl Immunology. 11:e1366. PubMed
  216. Meng YM, et al. 2021. Nat Commun. 12:6011. PubMed
  217. Zhu QY, et al. 2021. Nat Commun. 12:6624. PubMed
  218. Patel M, et al. 2021. Cancer Res. 81:3985. PubMed
  219. Shi X, et al. 2021. Nat Commun. 12:2547. PubMed
  220. Wen J, et al. 2017. J Hematol Oncol. 10.1186/s13045-017-0489-9. PubMed
  221. Ahmad S,et al. 2017. Prog Neuropsychopharmacol Biol Psychiatry. . 10.1016/j.pnpbp.2017.10.001. PubMed
  222. Hang J, et al. 2019. Cancer Med. 1.254166667. PubMed
  223. Tan X, et al. 2019. Sci Rep. 2.7125. PubMed
  224. Lienemann PS, et al. 2020. Adv Sci (Weinh). 7:1903395. PubMed
  225. Zhao J, et al. 2013. Elife. 21:537. PubMed
  226. Weber G, et al. 2015. Surgery. 158: 248-254. PubMed
  227. Jiang H, et al. 2016. Nat Med. 10.1038/nm.4123. PubMed
  228. Elgueta R, et al. 2016. J Immunol. 197: 3970 - 3981. PubMed
  229. Sharma A, et al. 2020. Cell. 183(2):377-394.e21. PubMed
  230. Hills LB, et al. 2021. J Immunol. 206:89. PubMed
  231. Page N, et al. 2021. Nat Commun. 12:1009. PubMed
  232. Pace J, et al. 2022. J Biomed Opt. 27:. PubMed
  233. Pedicona F, et al. 2022. Sci Signal. 15:eabl7989. PubMed
  234. Fidalgo MF, et al. 2022. Life Sci Alliance. 5:. PubMed
  235. Stirling ER, et al. 2022. J Immunother Cancer. 10:. PubMed
  236. Schweppe RE, et al. 2019. Mol Cancer Res. 17:1036. PubMed
  237. Yamada KJ, et al. 2020. PLoS Pathog. 16:e1008354. PubMed
  238. Yang A, et al. 2018. Cancer Discov. 0.525. PubMed
  239. Rentsendorj A, et al. 2017. Brain Behav Immun. 10.1016/j.bbi.2017.08.019. PubMed
  240. Castoldi F, et al. 2020. Cell Death Discov. 0.339583333. PubMed
  241. Wang L, et al. 2020. EMBO J. 39:e104514. PubMed
  242. Morris C, et al. 2017. MBio. 10.1128/mBio.00036-17. PubMed
  243. Theurl I, et al. 2016. Nat Med. 10.1038/nm.4146. PubMed
  244. Yan Q, et al. 2022. Clin Transl Med. 12:e1084. PubMed
  245. Zhai K, et al. 2021. Nat Cancer. 2:1136. PubMed
  246. Zhao D, et al. 2020. Cancer Discov. 10:1374. PubMed
  247. Dyck S, et al. 2018. J Neuroinflammation. 0.6875. PubMed
  248. Yang YS, et al. 2020. Mol Ther Methods Clin Dev. 17:922. PubMed
  249. Gangwar RS, et al. 2020. Sci Rep. 10:14348. PubMed
  250. Varga T, et al. 2013. J Immunol. 191:5695. PubMed
  251. Samuelson D, et al. 2016. J Immunol. 196: 2655 - 2665. PubMed
  252. Tsao P, et al. 2016. Proc Natl Acad Sci U S A. 113: 8242 - 8247. PubMed
  253. Soncini M, et al. 2016. Proc Natl Acad Sci U S A. 113: E6219 - E6227. PubMed
  254. Yang C, et al. 2016. Sci Rep. 6:34643. PubMed
  255. Nowyhed H, et al. 2017. Sci Rep. 7:40273. PubMed
  256. De Domenico E, et al. 2020. STAR Protoc. 1:100233. PubMed
  257. Wang Z, et al. 2020. Cell Rep. 33:108472. PubMed
  258. Sadik A, et al. 2020. Cell. 1252:182. PubMed
  259. Hiza H, et al. 2021. Sci Rep. 11:13190. PubMed
  260. Ito T, et al. 2021. Nat Commun. 12:2232. PubMed
  261. Hollerbach A, et al. 2021. J Thromb Haemost. . PubMed
  262. Gamrekelashvili J, et al. 2021. Bio Protoc. 11:e4007. PubMed
  263. Yang Z, et al. 2021. Nat Commun. 12:4852. PubMed
  264. Zhang Y, et al. 2022. Vaccines (Basel). 10: . PubMed
  265. Majumdar D, et al. 2021. Curr Protoc. 1:e216. PubMed
  266. Michelotti TC, et al. 2022. Front Cell Dev Biol. 10:1025240. PubMed
  267. Ren X, et al. 2022. STAR Protoc. 3:101818. PubMed
  268. Gladow N, et al. 2020. PLoS One. 15:e0227734. PubMed
  269. Ramachandran P, et al. 2019. Nature. 575:512. PubMed
  270. Vasamsetti SB, et al. 2018. Immunity. 49:93. PubMed
  271. Bowers J, et al. 2017. Front Immunol. 10.3389/fimmu.2017.01221. PubMed
  272. Zhang J, et al. 2020. Pain. 161:1381. PubMed
  273. Heim CE, et al. 2020. Nature Microbiology. 5(10):1271-1284. PubMed
  274. Canali S, et al. 2017. Blood. 129(4):405-414. PubMed
  275. Shen X, et al. 2016. Sci Rep. 6:38778. PubMed
  276. Sauter K, et al. 2016. Am J Physiol Gastrointest Liver Physiol. 311: G533 - G547. PubMed
  277. Song C, et al. 2015. Blood. 126: 1813 - 1822. PubMed
  278. Sasaki Y, et al. 2015. PLoS One. 10: 0131176. PubMed
  279. Feng D, et al. 2022. Nat Commun. 13:5554. PubMed
  280. Saber M, et al. 2021. J Neurosci Res. 99:1136. PubMed
  281. Xiang Y, et al. 2022. Skelet Muscle. 12:19. PubMed
  282. Yamazaki T, et al. 2021. Oncoimmunology. 10:1962592. PubMed
  283. Ansari M, et al. 2017. Cell Signal. 10.1016/j.cellsig.2017.04.014. PubMed
  284. Ahmad S, et al. 2017. Prog Neuropsychopharmacol Biol Psychiatry. 10.1016/j.pnpbp.2017.06.034. PubMed
  285. Bichsel C, et al. 2017. Sci Rep. 10.1038/s41598-017-09928-1. PubMed
  286. James M, et al. 2017. J Nucl Med. . 10.2967/jnumed.117.189597. PubMed
  287. Annibaldi A, et al. 2018. Mol Cell. 69:566. PubMed
  288. Hill SJ, et al. 2018. Cancer Discov. 1.308333333. PubMed
  289. Ma D, et al. 2013. J Virol. 87:3361. PubMed
  290. Kraft P, et al. 2013. Stroke. 44:3202. PubMed
  291. Kim M, et al. 2014. Mol Pharmacol. 86:284. PubMed
  292. Cekic C, et al. 2014. Cancer Res. 74:7250. PubMed
  293. Pankova D, et al. 2016. Mol Cancer Res. 14: 287 - 295. PubMed
  294. Kobia FM, et al. 2020. PLoS Biol. 18:e3000850. PubMed
  295. Tulasi DY, et al. 2021. Cell Mol Gastroenterol Hepatol. 11:1437. PubMed
  296. Hoffmann J, et al. 2021. Nat Commun. 12:3964. PubMed
  297. Xue X, et al. 2021. Cent Eur J Immunol. 46:191. PubMed
  298. Unsinger J, et al. 2021. J Leukoc Biol. 109:697. PubMed
  299. Yang YS, et al. 2022. Nat Commun. 13:6175. PubMed
  300. Resende M, et al. 2010. Physiol Genomics. 42:437. PubMed
  301. McLaughlin S, et al. 2019. Nat Commun. 3.795833333. PubMed
  302. Koury J, et al. 2019. PLoS One. 14:e0218670. PubMed
  303. Zhang Z, et al. 2017. Journal of Translational Medicine. 10.1186/s12967-017-1196-6. PubMed
  304. Peng X, et al. 2021. Stem Cell Res Ther. 198:12. PubMed
  305. Linder A, et al. 2020. EMBO J. 39:e105071. PubMed
  306. Muñoz-Ruiz M, et al. 2016. Nat Immunol. 10.1038/ni.3424. PubMed
  307. Chang Y, et al. 2014. Cytotherapy. 16:560. PubMed
  308. Hsia B, et al. 2012. J Allergy Clin Immunol. 130:205. PubMed
  309. Li N, et al. 2022. Nat Commun. 13:7281. PubMed
  310. Soileau AM, et al. 2022. Vet Sci. 9:. PubMed
  311. McClellan BL, et al. 2022. STAR Protoc. 3:101357. PubMed
  312. Governa V, et al. 2022. Proc Natl Acad Sci U S A. 119. PubMed
  313. Deng Y, et al. 2021. Nat Commun. 12:7041. PubMed
  314. Mai CL, et al. 2021. Theranostics. 11:1059. PubMed
  315. Chen P, et al. 2021. Clin Transl Med. 11:e462. PubMed
  316. Mochizuki M,et al. 2017. J Am Heart Assoc. . 10.1161/JAHA.117.005920. PubMed
  317. Roh–Johnson M, et al. 2017. Dev Cell. 43:549. PubMed
  318. Müller C, et al. 2018. Elife. 7:e34985. PubMed
  319. Guo W, et al. 2013. J Gerontol A Biol Sci Med Sci. . PubMed
  320. Liu Y, et al. 2015. Sci Rep. 5: 15134. PubMed
  321. Hodson D, et al. 2016. Proc Natl Acad Sci U S A. 113: 2039-2046. PubMed
  322. Munson D, et al. 2016. Proc Natl Acad Sci U S A. 113: 8272 - 8277. PubMed
  323. Ubellacker J, et al. 2017. Breast Cancer Res. 10.1186/s13058-017-0815-8. PubMed
  324. DU T, et al. 2020. Front Immunol. 1.571527778. PubMed
  325. Trial J, et al. 2020. Geroscience. . PubMed
  326. Cordo' V, et al. 2022. Nat Commun. 13:1048. PubMed
  327. Hallisey M, et al. 2022. STAR Protoc. 3:101163. PubMed
  328. Denoth-Lippuner A, et al. 2021. Cell Stem Cell. 28:2020. PubMed
  329. Maas RR, et al. 2021. Nat Protoc. 16:4692. PubMed
  330. Nagree MS, et al. 2022. EMBO Mol Med. 14:e14297. PubMed
  331. Sobecki M, et al. 2022. Cell Stem Cell. 29:1459. PubMed
  332. Denning W, et al. 2011. PLoS One. 6:e16897. PubMed
  333. Weaver J, et al. 2017. Part Fibre Toxicol. 10.1186/s12989-017-0206-4. PubMed
  334. Samuelson D, et al. 2017. PLoS Pathogens. 13(6):e1006426. PubMed
  335. Wong C, et al. 2017. J Immunol. 10.4049/jimmunol.1700397. PubMed
  336. Saber M, et al. 2020. Eur J Neurosci. 52:2791. PubMed
  337. Wu R, et al. 2020. J Immunother Cancer. 8:00. PubMed
  338. Yu H, et al. 2016. Sci Rep. 6:35190. PubMed
  339. Now H, Yoo J 2016. Cell Mol Immunol. 10.1038/cmi.2016.32. PubMed
  340. Bosma M, et al. 2016. Nat Commun. 7: 11314. PubMed
  341. YC S, SJ L 2015. Sci Rep. 5: 12578. PubMed
  342. Szulzewsky F, et al. 2015. PLoS One. 10:116644. PubMed
  343. Wu C, et al. 2012. PLoS One. 7:e42363. PubMed
  344. Visweshwaran SP, et al. 2022. Front Cell Dev Biol. 10:1008898. PubMed
  345. Li Q, et al. 2022. Signal Transduct Target Ther. 7:222. PubMed
  346. Fielding C, et al. 2022. Nat Commun. 13:543. PubMed
  347. Vechetti IJ, et al. 2021. FASEB J. 35:e21644. PubMed
  348. Gupta R, et al. 2020. Cell Stem Cell. 26:527. PubMed
  349. Roybal J, et al. 2011. Mol Cancer Res. 9:25. PubMed
  350. Cekic C, et al. 2013. J Exp Med. 210:2693. PubMed
  351. Barcia Durán JG, et al. 2021. Commun Biol. 4:406. PubMed
  352. Park HJ, et al. 2021. Int J Mol Sci. 22:. PubMed
  353. Cebrian-Silla A, et al. 2021. Elife. 10:. PubMed
  354. Yeini E, et al. 2021. Nat Commun. 12:1912. PubMed
  355. Wang X, et al. 2021. Sci Transl Med. 13:. PubMed
  356. Spiljar M, et al. 2021. Cell Metab. 33:2231. PubMed
  357. Kucykowicz S, et al. 2022. STAR Protoc. 3:101356. PubMed
  358. Kim TW, et al. 2022. Mol Ther Oncolytics. 26:1. PubMed
  359. Kathania M, et al. 2022. Cell Rep. 40:111345. PubMed
  360. Shaashua L, et al. 2022. Nat Commun. 13:6513. PubMed
  361. Field-Corbett C, et al. 2009. Stem Cells. 27:662. PubMed
  362. Zeng Y, et al. 2019. FASEB J. 33:6596. PubMed
  363. Zheng W, et al. 2019. Cell Rep. 29:1747. PubMed
  364. Tomas C, et al. 2019. Sci Rep. 9:11464. PubMed
  365. Kim JK, et al. 2019. Bioengineering (Basel). 6:24. PubMed
  366. Alizadeh A, et al. 2018. J Neuroinflammation. 15:53. PubMed
  367. Guo P, et al. 2017. J Clin Invest. 127:4242. PubMed
  368. Tsai SC, et al. 2017. Biomicrofluidics. 11:034122. PubMed
  369. Chen S, et al. 2020. Cancer Immunol Res. 1064:8. PubMed
  370. Erady C, et al. 2021. NPJ Genom Med. 6:04. PubMed
  371. de Silva S, et al. 2020. Cancer Immunol Res. 0.493055556. PubMed
  372. Wan C, et al. 2021. Cancer Res. 81:158. PubMed
  373. Wang X, et al. 2016. J Virol. 90: 10247 - 10258. PubMed
  374. Mohammed R, et al. 2016. Cell Rep. 14:760-771. PubMed
  375. Durward-Diioia M, et al. 2015. Infect Immun . 83: 4759 - 4771. PubMed
  376. Birnie K, et al. 2015. Mol Cancer Res. 13: 1106 - 1118. PubMed
  377. Liu X, et al. 2013. Invest Ophthalmol Vis Sci . 54:7386. PubMed
  378. Roy IM, et al. 2021. Bio Protoc. 11:e4130. PubMed
  379. Alam A, et al. 2022. STAR Protoc. 3:101563. PubMed
  380. Xiang Y, et al. 2022. Exp Mol Med. 54:1011. PubMed
  381. Guo Z, et al. 2022. Neurobiol Pain. 12:100096. PubMed
  382. Guo P, et al. 2022. Nat Cell Biol. 24:99. PubMed
  383. Wahane S, et al. 2021. Sci Adv. 7:. PubMed
  384. Xie Z, et al. 2020. Light Sci Appl. 9:161. PubMed
  385. Huang N, et al. 2021. Nat Med. 27:892. PubMed
  386. Song X, et al. 2021. FEBS Open Bio. 11:2453. PubMed

Antigen Details

Cell Type
Tregs
Gene ID
NA

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 06.10.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account