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Spark Blue™ 515 anti-human CD57 Antibody

Pricing & Availability
Clone
HNK-1 (See other available formats)
Regulatory Status
RUO
Other Names
HNK-1, NK-1, Leu-7
Isotype
Mouse IgM, κ
Ave. Rating
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Product Citations
publications
HNK1_SparkBlue515_CD57_Antibody_060723
Human peripheral blood cells were surface stained with anti-human CD8 APC and anti-human CD57 (clone HNK-1) Spark Blue™ 515 (left) or stained with anti-human CD8 APC only (right). Data shown were gated on lymphocyte populations.
  • HNK1_SparkBlue515_CD57_Antibody_060723
    Human peripheral blood cells were surface stained with anti-human CD8 APC and anti-human CD57 (clone HNK-1) Spark Blue™ 515 (left) or stained with anti-human CD8 APC only (right). Data shown were gated on lymphocyte populations.
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359627 25 tests 137€
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359628 100 tests 296€
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Description

CD57, also known as HNK-1, NK-1, and Leu-7 is a 100-115 kD oligosaccharide antigenic determinant expressed on a variety of proteins, lipids, and chondroitin sulfate proteoglycans. CD57 is expressed on a subset of peripheral blood lymphocytes, including NK cells and CD8+ T cells, and is also expressed on neural cells and striated muscle. CD57 is not expressed on red blood cells, granulocytes, monocytes, or platelets. While the function of CD57 is unknown, binding to L-selectin, P-selectin, and a fragment of laminin suggests that CD57 may be involved in cell-matrix interactions. CD57 is increased in some disease states associated with CD4/CD8 imbalances (AIDS, autoimmune disease, viral infections, and allograft transplants).

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Membrane extract of human lymphoblastoid cell line HSB-2.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Spark Blue™ 515 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Spark Blue™ 515 has a maximum excitation of 490 nm and a maximum emission of 515 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications for the relevant formats include: Western blotting1.

Application References

(PubMed link indicates BioLegend citation)
  1. Yoshihara Y, et al. 1991. J. Cell Biol. 115:731. (WB)
  2. Abo T, et al. 1981. J. Immunol. 127:1024.
  3. Abo T, et al. 1982. J. Immunol. 129:1752.
  4. Abo T, et al. 1982. J. Immunol. 129:1758.
RRID
AB_3083347 (BioLegend Cat. No. 359627)
AB_3083347 (BioLegend Cat. No. 359628)

Antigen Details

Structure
Oligosaccharide antigenic determinant present on a variety of proteins, lipids, and chrondroitin sulfate proteoglycans, 100-115 kD. The antigen is conserved across species.
Distribution

CD57 antigen is expressed on a subset of peripheral blood lymphocytes including a subset of NK cells and a subset of CD8+ T cells. Also expressed on neural cells and striated muscle.

Function
Unknown function, may be involved in cell-matrix interactions.
Ligand/Receptor
Binds to L-selectin and P-selectin in a calcium-dependent manner, also binds to second globular domain of E8 laminin fragment.
Cell Type
Lymphocytes, NK cells, T cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Schubert J, et al. 1989. In Leucocyte Typing IV (Knapp W, ed) Oxford University Press Oxford pp 711-714.
2. Palmer BE, et al. 2005. J. Immunol. 175:8415.
3. Schachner M, et al. 1995. Trends Neurosci. 18:183.
4. Wood KL, et al. 2005. Clin. Immunol. 117:294.

Gene ID
27087 View all products for this Gene ID
UniProt
View information about CD57 on UniProt.org
Go To Top Version: 1    Revision Date: 06.07.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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